miR-214-5p Targets ROCK1 and Suppresses Proliferation and Invasion of Human Osteosarcoma Cells

Zhang, Minglei; Wang, Dapeng; Zhu, Tianqi; Yin, Ruofeng

doi:10.3727/096504016x14719078133401

Cited by 45 publications

(42 citation statements)

References 24 publications

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“…We found miR-214-5p expression was significantly lower in HCC tissues when compared with that in matched normal tissues, which was the same as the expression status of miR-214-5p in human osteosarcoma. 32 Also, we found miR-214-5p overexpression could inhibit cell proliferation, migration, and induce cell cycle arrested in G0/G1, indicating the miR-214-5p may play a tumor suppressor role in the development and progression of HCC. Following, we identified the targeting gene of miR-214-5p in regulating cell proliferation, cell migration, and cell cycle since it is widely recognized that miRNA exerts its biological function through regulating the expression of its targets.…”

Section: Discussionmentioning

confidence: 67%

“…Also, we found miR‐214‐5p overexpression could inhibit cell proliferation, migration, and induce cell cycle arrested in G0/G1, indicating the miR‐214‐5p may play a tumor suppressor role in the development and progression of HCC. Following, we identified the targeting gene of miR‐214‐5p in regulating cell proliferation, cell migration, and cell cycle since it is widely recognized that miRNA exerts its biological function through regulating the expression of its targets . Therefore, by bioinformatic analysis and luciferase activity assay, we demonstrated KLF5 might be a potential target of miR‐214‐5p in HCC.…”

Section: Discussionmentioning

confidence: 99%

“…miR‐214‐5p is a product of the 110 bp miR‐214 gene in the intron of the Dynamin‐3 gene on human Chromosome 1‐NC_000001.10 . Very recently, Zhang et al demonstrated that miR‐214‐5p was downregulated and regulates cell proliferation and invasion by targeting ROCK1 in human osteosarcoma . However, the expression and clinical significance of miR‐214‐5p in HCC has not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

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miR‐214‐5p targets KLF5 and suppresses proliferation of human hepatocellular carcinoma cells

Pang

Wang

et al. 2018

J of Cellular Biochemistry

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MicroRNAs (miRNAs) are small endogenous conserved RNAs regulating genes expression through base pairing with the 3'-untranslated region (3'-UTR) of target messenger RNAs. MiR-214-5p is a newly identified miRNA with its biological role largely unknown. In this study, we explored miR-214-5p expression status in 78 paired tumor and nontumor tissues obtained from patients with hepatocellular carcinoma (HCC) by RT-qPCR. The effects of miR-214-5p expression on HCC cell proliferation, cell cycle progression, and cell migration were measured by CCK-8 assay, flow cytometry, and wound-healing assay. A dual-luciferase activity assay was performed to identify whether KLF5 was a target of miR-214-5p. Kaplan-Meier curve and log-rank test were used to investigate the effects of miR-214-5p and KLF5 on overall survival and disease-free survival of patients with HCC. We found miR-214-5p expression was sharply reduced in HCC tissues and cell lines compared with the normal tissues and cell lines. Functional assay revealed that miR-214-5p overexpression could downregulate cell proliferation, cell migration, and arrested cell cycle at G0/G1 phase. Further, we validated Krüppel-like factor 5 (KLF5) as a direct target of miR-214-5p, and was upregulated in HCC and inversely correlated with the expression of miR-214-5p. Moreover, we found the low expression of miR-214-5p and high expression of KLF5 were correlated with tumor size, tumor stage, and poorer 5-year overall survival and disease-free survival of patients with HCC. In conclusion, our results suggested miR-214-5p functions as a tumor suppressor through targeting KLF5 in HCC. Also, miR-214-5p and KLF5 were identified as potential prognostic markers and might be therapeutic targets in HCC.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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miR‐214‐5p targets KLF5 and suppresses proliferation of human hepatocellular carcinoma cells

Pang

Wang

et al. 2018

J of Cellular Biochemistry

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“…Among them, microRNAs gain many attention for their function as gene expression regulators. Just recently, miR-214-5p has been found to influence tumor process [15]. However, the effect of miR-214-5p on HCC remains unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, in serum, Elemeery MN, et al found miR-214-5p could be considered as early biomarkers for tracking the progress of liver fibrosis to HCC [14]. Zhang, et al showed that miR-214-5p suppresses proliferation and invasion of human osteosarcoma cells by targeting ROCK1 [15]. Li et al found in osteoblastic MC3T3-E1 cells, inhibition of microRNA-214-5p promotes survival and extracellular matrix formation [16].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-214-5p Inhibits the Invasion and Migration of Hepatocellular Carcinoma Cells by Targeting Wiskott-Aldrich Syndrome Like

Wang

Ren³

2018

Cell Physiol Biochem

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Background/Aims: This study aims to explore the effects of microRNA-214-5p (miR-214-5p) on the invasion and migration of Hepatocellular Carcinoma cells (HCC). Methods: Hepatocellular Carcinoma tissues and adjacent normal tissues from 44 hepatocellular carcinoma patients were prepared for this study. The HepG2 and BEL-7402 cells were transfected with miR-214-5p mimic and inhibitor. qRT-PCR was performed to detect the expressions of miR-214-5p. Transwell assays were used to detect the invasion and migration assays in HepG2 and BEL-7402 cells. A dual-luciferase reporter assay was conducted to examine the effect of miR-214-5p on Wiskott-Aldrich Syndrome Like (WASL/ N-WASP). Western blot and qRT-PCR were used to measure the expressions of the E-cadherin, N-cadherin and Vimentin proteins. Transwell chamber assays were performed to detect cell invasion and migration. Results: Compared with normal tissues, HCC tissues demonstrated significantly lower expression of miR-214-5p. Overexpression of miR-214-5p significantly inhibited the migration and invasion of HCC cells and inhibition of miR-214-5p promoted the migration and invasion. Additionally, miR-214-5p suppressed the epithelial-mesenchymal transition (EMT). Further study showed WASL was a putative target gene of miR-214-5p. Up-regulating the expression of WASL could reverse the inhibition effect of miR-214-5p on invasion and migration. Conclusion: Our data suggested that miR-214-5p inhibited the invasion and migration of HepG2 and BEL-7402 by targeting WASL in Hepatocellular carcinoma.

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LncRNA TDRG1/miR‐214‐5p axis affects preeclampsia by modulating trophoblast cells

Gong

Cheng

Shi

et al. 2019

Cell Biochemistry & Function

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Because of limited treatment options, preeclampsia (PE) is the leading cause of perinatal morbidity and mortality worldwide. Recently, lncRNA TDRG1 is reported to be aberrantly down-regulated in PE placenta, and the abnormal expression of TDRG1 might play a key or partial role in PE development. In this study, we found that TDRG1 was significantly down-regulated in PE placenta compared with the normal placenta.The cell proliferation, migration, invasion, and cell cycle were explored by CCK-8, wound-healing, transwell, and flow cytometer assay, respectively. Experimental results showed that TDRG1 accelerated the proliferation, migration, and invasion of trophoblast cells. Dual-luciferase reporter assays confirmed that TDRG1 could bind to miR-214-5p. Besides, knockdown of TDRG1 suppressed the cell proliferation, migration, and invasion, while knockdown of miR-214-5p reversed the effect. Jagged1 and Notch1 were negatively regulated by miR-214-5p while positively modulated by TDRG1. In conclusion, TDRG1 promoted trophoblast cells viability and invasion by negatively regulating miR-214-5p expression, contributing to a better understanding of PE pathogenesis and providing new light on TDRG1-directed diagnosis and treatment.Significance of the study: In this work, we observed that TDRG1 was able to promote cell proliferation, migration, and invasion cells by suppressing the expression of miR-214-5p and regulating the Notch signalling pathway in trophoblast cells. As far as we know, the effect of TDRG1/miR-214-5p axis on cell viability, migration, and invasion of trophoblast cells was firstly introduced. Our findings provided a better understanding of the mechanism of PE. Moreover, it is reasonable to believe that TDRG1 may be employed as a strategy to treat PE in the future. K E Y W O R D SmiR-214-5p, Notch1, preeclampsia, TDRG1, trophoblast cells

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miR-214-5p Targets ROCK1 and Suppresses Proliferation and Invasion of Human Osteosarcoma Cells

Cited by 45 publications

References 24 publications

miR‐214‐5p targets KLF5 and suppresses proliferation of human hepatocellular carcinoma cells

miR‐214‐5p targets KLF5 and suppresses proliferation of human hepatocellular carcinoma cells

MicroRNA-214-5p Inhibits the Invasion and Migration of Hepatocellular Carcinoma Cells by Targeting Wiskott-Aldrich Syndrome Like

LncRNA TDRG1/miR‐214‐5p axis affects preeclampsia by modulating trophoblast cells

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