miR-20a is an independent prognostic factor in colorectal cancer and is involved in cell metastasis

Zhang, Guang‐Jun; Li, Yu; Zhou, He; Xiao, Hua-xu; Zhou, Tong

doi:10.3892/mmr.2014.2144

Cited by 40 publications

(28 citation statements)

References 34 publications

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“…DNAJB4 (DnaJ heat shock protein family member B4) is a chaperone with a strong tumor suppressor effect in CRC whose down-regulation has been associated with patient outcome [42]. In this regard, miR-20a has also been shown to induce EMT, to regulate the migration and invasion of SW480 cells [43] and to contribute to an increased chemoresistance of CRC [44]. Of note, here we did not find any (inverse) correlation between the levels of expression of miR-20a and the mRNA levels of other genes reported to be altered IN parallel in CRC such as the PCSK5, RNF152 and SOCS2 genes [45–47].…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization of liver metastases from colorectal cancer patients

et al. 2016

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Metastatic dissemination is the most frequent cause of death of sporadic colorectal cancer (sCRC) patients. Genomic abnormalities which are potentially characteristic of such advanced stages of the disease are complex and so far, they have been poorly described and only partially understood. We evaluated the molecular heterogeneity of sCRC tumors based on simultaneous assessment of the overall GEP of both coding mRNA and non-coding RNA genes in primary sCRC tumor samples from 23 consecutive patients and their paired liver metastases. Liver metastases from the sCRC patients analyzed, systematically showed deregulated transcripts of those genes identified as also deregulated in their paired primary colorectal carcinomas. However, some transcripts were found to be specifically deregulated in liver metastases (vs. non-tumoral colorectal tissues) while expressed at normal levels in their primary tumors, reflecting either an increased genomic instability of metastatic cells or theiradaption to the liver microenvironment. Newly deregulated metastatic transcripts included overexpression of APOA1, HRG, UGT2B4, RBP4 and ADH4 mRNAS and the miR-3180-3p, miR-3197, miR-3178, miR-4793 and miR-4440 miRNAs, together with decreased expression of the IGKV1-39, IGKC, IGKV1-27, FABP4 and MYLK mRNAS and the miR-363, miR-1, miR-143, miR-27b and miR-28-5p miRNAs. Canonical pathways found to be specifically deregulated in liver metastatic samples included multiple genes related with intercellular adhesion and the metastatic processes (e.g., IGF1R, PIK3CA, PTEN and EGFR), endocytosis (e.g., the PDGFRA, SMAD2, ERBB3, PML and FGFR2), and the cell cycle (e.g., SMAD2, CCND2, E2F5 and MYC). Our results also highlighted the activation of genes associated with the TGFβ signaling pathway, -e.g. RHOA, SMAD2, SMAD4, SMAD5, SMAD6, BMPR1A, SMAD7 and MYC-, which thereby emerge as candidate genes to play an important role in CRC tumor metastasis.

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Section: Discussionmentioning

confidence: 99%

Genomic characterization of liver metastases from colorectal cancer patients

et al. 2016

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“…It has been shown that the expression of miR-17-92 cluster genes are increased in CD34 + cells in the early chronic phase of CML through the BCR-ABL-c-MYC -miR-17-92 pathway (Venturini et al, 2007). miR-20a belongs to the miR-17-92 family and is abnormally expressed in various tumors, such as lung cancer, gastric cancer, and colorectal cancer (Zhang et al, 2014; Fuziwara and Kimura, 2015; da Silva Oliveira et al, 2016). The high miR-20a expression can attenuate the expression of PTEN to promote tumor development (Fuziwara and Kimura, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The high miR-20a expression can attenuate the expression of PTEN to promote tumor development (Fuziwara and Kimura, 2015). miR-20a is closely associated with the growth, invasion, and metastasis of tumor cells (Huang et al, 2012; Qiang et al, 2014; Zhang et al, 2014; Wang et al, 2015; Zhao et al, 2015). The study by Zhang et al (2014) showed that the expression of miR-20a increased in colorectal cancer and was closely associated with the lymph node and distant metastases.…”

Section: Introductionmentioning

confidence: 99%

“…miR-20a is closely associated with the growth, invasion, and metastasis of tumor cells (Huang et al, 2012; Qiang et al, 2014; Zhang et al, 2014; Wang et al, 2015; Zhao et al, 2015). The study by Zhang et al (2014) showed that the expression of miR-20a increased in colorectal cancer and was closely associated with the lymph node and distant metastases. In addition, high miR-20a expression could induce tumor cell resistance to chemotherapeutic drugs through different target genes (Chai et al, 2011; Zhu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Antisense miRNA-20a Alone or in Combination with Imatinib on K562 Cell Proliferation

Zhou

Zeng

et al. 2017

Front. Pharmacol.

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Objective: The effects of microRNA-20a (miR-20a) antisense oligonucleotides (ASODNs) on the proliferation and apoptosis of K562 cells were investigated, and the effects of these ASODNs in combination with imatinib on K562 cells were preliminarily observed.Methods: miR-20a ASODNs and scrambled oligonucleotides (SODNs) were chemically synthesized, and the later was used as the control. miR-20a ASODNs were transfected into K562 cells using Lipofectamine 2000 transfection reagent, and the expression of miR-20a was detected using real-time quantitative RT-PCR (qRT-PCR). The CCK8 assay was performed to detect the inhibition of the cell growth rate. The cells were stained by Hoechst 33258 to detect apoptotic cell morphology. Annexin V/PI double staining was used to detect the cell apoptosis rate using flow cytometry. The protein expression levels of E2F1, P21, and Bim in the K562 cell line were detected using western blotting.Results: The qRT-PCR results showed that the expression level of miR-20a in K562 cells transfected with miR-20a ASODNs was lower than those in the normal control, SODN and blank transfection groups (p < 0.05). miR-20a ASODNs significantly inhibited the growth of K562 cells as compared to the controls (p < 0.05). The Hoechst staining results showed morphological changes, suggesting apoptosis. The cell apoptosis rates in the ASODN group was (13.9 ± 1.5)%, which was significantly higher than that in the normal control group (1.84 ± 0.21)%, blank transfection group (3.21 ± 0.32)%, and SODN group (3.72 ± 0.44)% (p < 0.05). The protein expression of E2F1 and P21 in K562 cells transfected with miR-20a ASODNs were higher, while the level of Bim protein was significantly lower than that in the control groups. When miR-20a ASODNs were combined with imatinib, the growth of K562 cells was significantly inhibited as compared to the ASODN treatment alone, imatinib alone, and SODN+imatinib groups (p < 0.05).Conclusions: miR-20a ASODNs could induce apoptosis and inhibit the proliferation of K562 cells. In addition, imatinib combined with miR-20a ASODNs can increase the inhibitory effect on K562 cell proliferation.

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“…Some of the selected studies investigated the target genes of the miRs associated with the prognostic aspects of CRC. In total, 65 target genes were analyzed in which the most frequent ones were: PTEN, investigated in six studies 29, 32, 33, 50, 60, 61 , SMAD4, investigated in three studies 62–64 , and TP53 65, 66 investigated in two studies.…”

Section: Resultsmentioning

confidence: 99%

Tissue micro-RNAs associated with colorectal cancer prognosis: a systematic review

Santos

Penna

Carneiro

et al. 2019

Preprint

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Colorectal cancer (CRC) is a multifactorial disease commonly diagnosed worldwide, with high mortality rates. Several studies demonstrate important associations between differential expression of micro-RNAs (miRs) and the prognosis of CRC. However, only a few systematic reviews emphasize the most relevant miRs able to contribute to the establishment of new prognostic biomarkers in CRC patients. The present study aimed to identify differentially expressed tissue miRs associated with prognostic factors in CRC patients, through a systematic review of the Literature. Using the PubMed database, Cochrane Library and Web of Science, studies published in English evaluating miRs differentially expressed in tumor tissue and significantly associated with the prognostic aspects of CRC were selected. All the included studies used RT-PCR (Taqman or SYBR Green) for miR expression analysis and the period of publication was from 2009 to 2018. A total of 115 articles accomplished the inclusion criteria and were included in the review. The studies investigated the expression of 102 different miRs associated with prognostic aspects in colorectal cancer patients. The most frequent oncogenic miRs investigated were miR-21, miR-181a, miR-182, miR-183, miR-210 and miR-224 and the hyperexpression of these miRs was associated with distant metastasis, lymph node metastasis and worse survival in patients with CRC. The most frequent tumor suppressor miRs were miR-126, miR-199b and miR-22 and the hypoexpression of these miRs was associated with distant metastasis, worse prognosis and a higher risk of disease relapse (worse disease-free survival). Specific tissue miRs are shown to be promising prognostic biomarkers in patients with CRC, given their strong association with the prognostic aspects of these tumors, however, new studies are necessary to establish the sensibility and specificity of the miRs in order to use them in clinical practice.

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miR-20a is an independent prognostic factor in colorectal cancer and is involved in cell metastasis

Cited by 40 publications

References 34 publications

Genomic characterization of liver metastases from colorectal cancer patients

Genomic characterization of liver metastases from colorectal cancer patients

The Effects of Antisense miRNA-20a Alone or in Combination with Imatinib on K562 Cell Proliferation

Tissue micro-RNAs associated with colorectal cancer prognosis: a systematic review

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