miR-205 promotes tumor proliferation and invasion through targeting ESRRG in endometrial carcinoma

Abstract

Increasing evidence suggests that miR‑205 is frequently dysregulated in many types of human cancers, suggesting its important roles in the initiation and progression of cancer. However, the functions of miR‑205 in human endometrial endometrioid carcinoma (EEC) are still unknown. In this study, we investigated the expression of miR‑205 in both normal endometrium and EEC tissues using TaqMan PCR. Compared to normal tissues, miR‑205 was significantly upregulated in EEC (P<0.001). After transfection of miR‑205 inhibitors into Ishikawa cells (or transfection of miR‑205 mimics into AN3CA cells), we demonstrated that the cellular proliferation, migration and invasion properties were negatively regulated by miR‑205. Moreover, by combination of microRNA target prediction algorithms and luciferase reporter system, we identified estrogen-related receptor‑γ (ESRRG) as a target of miR‑205. In conclusion, we demonstrated frequent upregulation of miR‑205 in EEC. In gain‑of‑function and loss‑of‑function assays, inhibition of miR‑205 reduced cellular proliferation, migration and invasion; vice versa, increased levels of miR‑205 led to upregulated cellular proliferation, migration and invasion. Nonetheless, we identified the ESRRG gene to be a novel target, which could be helpful to elucidate mechanisms underlying the tumorigenesis of EEC.

Tumor progression, metastasis, and modulators of epithelial–mesenchymal transition in endometrioid endometrial carcinoma: an update

“…Frequent upregulation of miR-205 has also been demonstrated in EEC. Inhibition of miR-205 reduced cellular proliferation, migration, and invasion (Su et al 2013). miR-194 was found to regulate EMT by suppressing BMI-1 in EC.…”

MiR-205 promotes motility of ovarian cancer cells via targeting ZEB1

“…Growing evidence shows that miR-205 plays a crucial role in a variety of tumors and functions as an oncogene or a tumor suppressor determined by the specific cancer context or its target genes. Especially in gynecological malignancies, miR-205 has been found to be dramatically upregulated in endometrial carcinoma tissues, and promote tumor proliferation and invasion through targeting estrogen-related receptor-γ, while inhibit cell apoptosis by targeting phosphatase and tensin homolog deleted on chromosome ten [27,28]; In ovarian cancer cells, the upregulation of miR-205 has been reported to induce cell invasion and proliferation [29]; The increased serum miR-205 level has also identified as a predictive biomarker for the prognosis of cervical cancer patients [30]. On the other hand, miR-205 is significantly down-regulated in gastric cancer tissues, and the inhibition of miR-205 can effectively promote gastric cancer cell proliferation via cell-cycle progression through repressing oncoprotein Yin Yang 1…”

miR-205 in Situ Expression and Localization in Head and Neck Tumors - a Tissue Array Study

“…miR-205 expression is dysregulated in many human cancers, influenced various cancer cell properties in cell lines and also regulated several major cancer pathways (Xie et al, 2012;Lei et al, 2013;Su et al, 2013;Wang et al, 2013). However, pattern of miR-205 expression in head and neck cancers demonstrated conflicting results.…”