miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN

Abstract

Non-small cell lung cancer (NSCLC) is one of the most common causes of cancer-related mortality worldwide. microRNAs (miRNAs) play critical roles in carcinogenesis. miR-205 has been shown to be upregulated in NSCLC. In the present study, we identified the promotive effects of miR-205 on various significant biological properties of NSCLC cells, and confirmed the regulation of PTEN by miR-205. The expression of miR-205 was examined by quantitative real-time PCR both in NSCLC cell lines and tissues. The effect of miR-205 on PTEN expression was assessed in NSCLC cell lines with miR-205 mimics/inhibitor to elevate/decrease miR-205 expression. Furthermore, the roles of miR-205 in regulating the biological properties of NSCLC cells, including growth, invasion and chemoresistance, were assayed using miR-205 mimic/inhibitor-transfected cells. The 3'-untranslated region (3'-UTR) of PTEN combined with miR-205 and this was confirmed by luciferase reporter assay and western blotting. miR-205 expression was increased in NSCLC cell lines as well as in tissues. Overexpression of miR-205 promoted growth, migration and invasion, and enhanced the chemoresistance of NSCLC cells. Luciferase activity and western blotting demonstrated that miR-205 negatively regulated PTEN at a posttranscriptional level. However, miR-205 knockdown suppressed these processes in A549 cells and increased the expression of PTEN protein. Furthermore, overexpression of PTEN exhibited effects identical with those of the miR-205 inhibitor in NSCLC cells. Our results demonstrated that miR-205 is involved in the tumorigenesis of NSCLC through modulation of the PTEN signaling pathway.

MicroRNA‑216b regulated proliferation and invasion of non‑small cell lung cancer by targeting SOX9

“…In addition, miRNAs have been revealed to act as tumor suppressors or oncogenes in the carcinogenesis and progression of NSCLC, and have been revealed to be involved in numerous aspects of cancer biology, including cell proliferation, the cell cycle, apoptosis, migration and invasion (28,29). Therefore, the identification of the cancer-specific miRNAs and corresponding direct target genes that are essential for NSCLC carcinogenesis and progression may provide promising therapeutic targets for patients with NSCLC.…”

miR-205 in Situ Expression and Localization in Head and Neck Tumors - a Tissue Array Study

“…miR-205 expression is dysregulated in many human cancers, influenced various cancer cell properties in cell lines and also regulated several major cancer pathways (Xie et al, 2012;Lei et al, 2013;Su et al, 2013;Wang et al, 2013). However, pattern of miR-205 expression in head and neck cancers demonstrated conflicting results.…”

MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2

“…7, 8 In NSCLC, multiple miRNAs, such as miR-10b, miR-150 and miR-205, were found to promote NSCLC carcinogenesis. 9, 10, 11 In contrast, miR-16, miR-140 and miR-223 have been identified as tumor suppressors. 7, 12, 13 In several cancers, including NSCLC, 14 miR-152 levels are decreased, whereas miR-152 functions as a tumor suppressor in cancers including prostate cancer, glioma and endometrial cancer.…”