miR-205 Expression Promotes Cell Proliferation and Migration of Human Cervical Cancer Cells

Abstract

MicroRNAs (miRNAs) are short non-coding RNA regulators that control gene expression mainly through post-transcriptional silencing. We previously identified miR-205 in a signature for human cervical cancer using a deep sequencing approach. In this study, we confirmed that miR-205 expression was frequently higher in human cervical cancer than their matched normal tissue samples. Functionally, we demonstrate that miR-205 promotes cell proliferation and migration in human cervical cancer cells. To further understand the biological roles of miR-205, we performed in vivo crosslinking and Argonaute 2 immunoprecipitation of miRNA ribonucleoprotein complexes followed by microarray analysis (CLIP-Chip) to identify its potential mRNA targets. Applying CLIP-Chip on gain- and loss-of-function experiments, we identified a set of transcripts as potential targets of miR-205. Several targets are functionally involved in cellular proliferation and migration. Two of them, CYR61 and CTGF, were further validated by Western blot analysis and quantification of mRNA enrichment in the Ago2 immunoprecipitates using qRT-PCR. Furthermore, both CYR61 and CTGF were downregulated in cervical cancer tissues. In summary, our findings reveal novel functional roles and targets of miR-205 in human cervical cancer, which may provide new insights about its role in cervical carcinogenesis and its potential value for clinical diagnosis.

MicroRNA-101 Inhibits Cell Proliferation, Invasion, and Promotes Apoptosis by Regulating Cyclooxygenase-2 in Hela Cervical Carcinoma Cells

“…These studies indicate that the dysregulation of miRNAs is frequently involved in carcinogenesis and cancer progression and, moreover, they suggest that the pathogenesis of cervical carcinoma may be attributed to miRNAs. In addition, miRNA expression profiling can also be used to predict the clinical outcome of cancer patients ( Jiang et al, 2008;Xie et al, 2012).…”

MicroRNA‐7 downregulates XIAP expression to suppress cell growth and promote apoptosis in cervical cancer cells

“…Previous reports from other labs and our lab have shown that many miRNAs are aberrantly expressed in cervical cancer and that miRNAs can act as tumor suppressors (e.g., miR-302-367, miR-424, miR-214) [9][10][11][12] or enhancers (e.g., miR-205, miR-21, miR-19a/b) [13][14][15]. Although some progress has been achieved in the past decades, much more effort is needed to elucidate the occurrence and mechanism of cervical cancer.…”

Cyr61 Expression Pattern and Association with Clinicopathological Factors in Patients with Cervical Cancer

“…In recent years the prognostic value of microRNAs in cervical carcinoma was subjected (23). In cervical carcinogenesis, Xie et al were able to identify a regulatory influence of miR-205 (microRNA-205) triggering Cyr61 down-regulation in CC tissues (24). This is the first study to evaluate the expression and clinical impact of Cyr61 in CC.…”