miR‑205‑5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1

Abstract

Many studies have demonstrated that miRNAs have influence on tumorigenesis and progression of human cancers, including invasion and migration. Thus, the role of miR-205/ZEB1 axis for the migration and invasion of prostate cancer cells was explored in the present study. The miR-205-5p and zinc finger E-box binding homeobox 1 (ZEB1) mRNA expression levels were observed in prostate cancer tissues or cell lines via reverse transcription-quantitative PCR (RT-qPCR), and the protein level of ZEB1 was measured by western blotting. Dual-Luciferase Reporter Assay was used to verify the relationship between miR-205-5p and ZEB1. In addition, cell migration and invasion was measured by Transwell assay. The results revealed that, compared with the control, downregulation of miR-205-5p was detected in prostate cancer tissues and cell lines, and miR-205-5p overexpression was found to inhibit cell migration and invasion. Moreover, miR-205-5p was confirmed to directly target ZEB1 in prostate cancer. Importantly, ZEB1 was identified to weaken the inhibitory effect of miR-205-5p in prostate cancer. In conclusion, miR-205-5p inhibited cell migration and invasion in prostatic carcinoma by targeting ZEB1 and miR-205-5p/ZEB1 axis shows potential to be developed in therapeutic strategies for prostate cancer.

Long non-coding RNA SNHG5 promotes glioma progression via miR-205/E2F3 axis

“…MiR-205 functions as a tumour suppressor in human cancers, such as breast cancer, prostatic carcinoma and oral squamous cell carcinoma [27][28][29]. Our study found that miR-205 suppressed the glucose uptake, migration and invasion of glioma cells.…”

Chemotherapeutic Stress Influences Epithelial–Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer

“…[64,65] Quantitative PCR analysis revealed that the selected microRNAs were reproducibly detectable at heterogeneous levels in all BCSC lines ( Figure S5A). In particular, miR-205-5p, which is well known to inhibit ZEB1 expression directly targeting its 3'UTR [66], was inversely expressed compared with ZEB1 in all BCSC lines ( Figure S2G and S5A). Throughout the chemotherapeutic stress and recovery processes, most miRNAs in question were differentially regulated ( Figure S5B,C).…”

Oxidative stress‐induced angiogenesis is mediated by miR‐205‐5p

“…7 In addition, we have identified some potential targets of miR-205-5p and their biological processes. 7,[11][12][13]47 Moreover, it has been suggested that miR-205-5p could interact indirectly via PI3K/AKT also involved in angiogenesis. In fact, it F I G U R E 5 VEGFA-mediated Vasculogenesis is regulated by miR-205-5p.…”

“…Statistically significant differences were set at *P < .05 is well documented that miR-205-5p acts as a tumour suppressor by regulating cell migration and proliferation. 7,[11][12][13]47 Moreover, it has been suggested that miR-205-5p could interact indirectly via PI3K/AKT also involved in angiogenesis. 13 In the present report, we have observed that VEGFA and miR-205-5p are inversely expressed ( Figure 3), inducing angiogenesis.…”