miR‑204‑5p promotes apoptosis and inhibits migration of gastric cancer cells by targeting HER‑2

Yang, Shu; Chen, Binni; Zhang, Baogen; Li, Caiping; Qiu, Yuena; Yang, Hanhui; Huang, Zhiwei

doi:10.3892/mmr.2020.11367

Cited by 15 publications

(15 citation statements)

References 38 publications

(48 reference statements)

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“…Notably, it has been documented that miR-204-5p affected the development of varied human tumors progression by regulating diverse genes such as runt-related transcription factor 2 (RUNX2) [17], human epidermal growth factor receptor 2 (HER [31], high mobility group B1 (HMGB1) [32], and RRM2 [33]. Among them, RRM2 is a catalytic subunit of ribonucleotide reductase that intensely involved in DNA synthesis [34].…”

Section: Discussionmentioning

confidence: 99%

Knockdown of lncRNA SNHG4 suppresses gastric cancer cell proliferation and metastasis by targeting miR-204-5p

Cheng¹,

Zhang²,

Zhu³

et al. 2021

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Long non-coding RNAs (lncRNAs) have been identified as critical regulators in gastric cancer (GC) progression. However, whether lncRNA small nucleolar host gene 4 (SNHG4) functions in GC development remains unknown. In this study, the bio-functional role of SNHG4 and its potential mechanism on GC progression were systematically dissected. To investigate the role of SNHG4 in GC, we silenced SNHG4 using short hairpin RNAs (shRNAs) to perform loss-of-function assays. The results showed that SNHG4 expression in GC cells was at a higher level compared to normal gastric mucosal epithelial cells. Knockdown of SNHG4 dramatically suppressed proliferation, migration and invasion, and blocked cell cycle progression of GC cells. Moreover, knockdown of SNHG4 upregulated microRNA-204-5p (miR-204-5p) expression, whereas downregulated ribonucleotide reductase subunit M2 (RRM2) expression in GC cells. Dua l-luciferase reporter assay results showed that miR-204-5p was a direct target of SN HG4. Additionally, knockdown of SHNG4 suppressed GC tumorigenesis in xenograft mouse models. Taken together, these data demonstrated that knockdown of SNHG4 suppressed GC development by targeting miR-204-5p.

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Section: Discussionmentioning

confidence: 99%

Knockdown of lncRNA SNHG4 suppresses gastric cancer cell proliferation and metastasis by targeting miR-204-5p

Cheng¹,

Zhang²,

Zhu³

et al. 2021

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“…Referring to many studies, miR-204-5p was concluded as a tumor suppressor factor. For example, it has been reported that miR-204-5p attenuates tumor malignancy from various levels in gastric cancer, prostate cancer, and hepatocellular cancer [ 3 – 6 ]. However, some researchers observed the converse results in other tumors; for example, Zhang and his colleagues summarized a tumorigenesis role of miR-204-5p in cervical cancer [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

miR-204-5p Hampers Breast Cancer Malignancy and Affects the Cell Cycle by Targeting PRR11

Shen

et al. 2022

Computational and Mathematical Methods in Medicine

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Purpose. To unravel mechanisms of miR-204-5p in breast cancer (BC) cells. Methods. miR-204-5p expression level in BC cell lines was measured by qRT-PCR. Putative binding sites of miR-204-5p on the 3 ′ -untranslated region of PRR11 were predicted by the bioinformatics method and verified by the dual-luciferase method. Protein and mRNA levels of PRR11 in BC were determined by western blot and qRT-PCR. The association between two genes was analyzed by correlation analysis. Cancer cell functions were evaluated through CCK8, flow cytometry, and Transwell approaches. Results. Significant downregulation of miR-204-5p was observed in BC tissue and cells. Cell functional experiments showed the inhibition of miR-204-5p on cell behaviors and cell cycle. PRR11 was the downstream target of miR-204-5p. Inhibition of RPP11 could reverse the impacts of the miR-204-5p inhibitor on cell functions of BC. Conclusion. Our study revealed that the miR-204-5p/PRPP11 axis suppressed BC progression, which may provide a novel insight into the regulatory roles of miR-204-5p.

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“…According to several studies, miRNAs are involved in the pathogenesis and development of GC, furthermore, an increasing number of miRNAs have been shown to be used as markers for diagnosis in GC treatment, and the dysregulated expression of miRNAs can remarkably affect prognosis [ 17 , 18 ]. For instance, miRNA-204-5p exerts antitumor effects by inhibiting GC cell proliferation, invasion and migration, and promoting apoptosis, suggesting that miRNA-204-5p could be a potential target for the treatment of GC [ 19 ]. Compared with other miRNAs, miRNA-381 is highly sensitive and specific in GC diagnosis.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-34c-5p exhibits anticancer properties in gastric cancer by targeting MAP2K1 to inhibit cell proliferation, migration, and invasion

Zhao

et al. 2022

BioMed Research International

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Purpose. Gastric cancer(GC)is one of the deadliest digestive tract tumors worldwide，existing studies suggest that dysregulated expression of microRNAs (miRNAs) plays an important role in the pathogenesis and progression of GC. This study aimed to investigate the expression, biological function, and downstream mechanism of miR-34c-5p in GC, provide new targets for gastric cancer diagnosis and treatment. Methods. The expression of miR-34c-5p in GC tissues and cell lines was examined by RT-qPCR. Cell wound healing, transwell and cell cloning assays were used to detect the effect of miR-34c-5p on the migration and invasion abilities, respectively, of GC cells. Western blot was performed to detect the expression of related proteins. Bioinformatics analysis was used to predict the binding of MAP2K1 to miR-34c-5p and the targeting relationship was confirmed by dual luciferase reporter assay. Results. The expression level of miR-34c-5p was significantly decreased in GC tissues and cell lines. miR-34c-5p overexpression inhibited migration, invasion, and colony formation of gastric cancer cells, the related protein E-cadherin expression was significantly increased and N-cadherin, vimentin, and PCNA expression were significantly decreased, while miR-34c-5p knockdown exerted the opposite effects. In addition, the targeting relationship between miR-34c-5p and MAP2K1 was predicted and confirmed, and further confirmed by rescue experiments that MAP2K1 alleviated the inhibitory effect of miR-34c-5p in GC. Conclusion. MiR-34c-5p is lowly expressed in GC, and it can target MAP2K1 to exert inhibitory effects on GC proliferation, invasion, and migration. These findings provide a promising biomarker and a potential therapeutic target for gastric cancer.

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miR‑204‑5p promotes apoptosis and inhibits migration of gastric cancer cells by targeting HER‑2

Cited by 15 publications

References 38 publications

Knockdown of lncRNA SNHG4 suppresses gastric cancer cell proliferation and metastasis by targeting miR-204-5p

Knockdown of lncRNA SNHG4 suppresses gastric cancer cell proliferation and metastasis by targeting miR-204-5p

miR-204-5p Hampers Breast Cancer Malignancy and Affects the Cell Cycle by Targeting PRR11

MicroRNA-34c-5p exhibits anticancer properties in gastric cancer by targeting MAP2K1 to inhibit cell proliferation, migration, and invasion

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