MiR-203a-3p, miR-204-3p, miR-222-3p as useful diagnostic and prognostic tool for thyroid neoplasia spectrum

Stojanović, Stefana; Dobrijević, Zorana; Šelemetjev, Sonja; Đorić, Ilona; Miljuš, Jelena Janković; Živaljević, Vladan; Denčić, Tijana Išić

doi:10.1007/s12020-022-03185-7

Cited by 3 publications

(9 citation statements)

References 39 publications

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“…25 Also, Dai et al detected significantly decreased levels of miRNA-203a-3p in PTC tissues and Stojanović et al found down-regulated miR-203a-3p indicates presence of either PTC/follicular thyroid adenoma with high sensitivity and specificity. 26,27 On contrary, the expression levels of miR-576 were upregulated in malignant than in benign FNAC and in aspirates of PTC and aspirates of other TC and were found to be more diagnostic than cellular findings. These data are in accordance with Hai et al who detected significant increase in the expression level of miR-576-5p in PTC tissues.…”

Section: Discussionmentioning

confidence: 96%

The diagnostic performance of microRNAs expression levels in cellular aspirate of solitary thyroid nodule overrides that of cytology

Hussein,

Hafez,

Marie

et al. 2023

Int Surg J

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Background: Evaluation of the diagnostic performance of estimated expression levels of microRNAs 203a, 206 and 576 in the cellular aspirate of solitary thyroid nodule (STN) in comparison to cytological examination for identification of malignant TN. Methods: The 74 patients with STN underwent clinical and US evaluation and gave blood samples for estimation of serum levels of thyroglobulin (TG) and anti-thyroglobulin antibodies (ATG). Then, fine-needle aspiration was performed to obtain cellular aspirate for cytological examination and estimation of levels of microRNAs. The appropriate surgical procedure was undertaken and the excised specimens were sent for pathological diagnosis. Results: Pathologically, 19 nodules had papillary thyroid carcinoma (PTC), 6 nodules had follicular carcinoma and one medullary carcinoma, while 48 nodules were benign nodules. Cytological examination diagnosed malignancy in 5 specimens, while specimens were suspicious of malignancy, 25 specimens were diagnosed as benign and 14 specimens as non-diagnostic. Serum levels of TG and ATG were significantly higher with malignancy. Expression levels of miR-203a and 206 were significantly lower, while miR-576 were significantly higher in malignant than benign aspirates. Multivariate regression analysis showed more significant ability for miR-576 level than cytological examination as screening and for miR-203a than miR-206 as diagnostic for malignant aspirate and for PTC Conclusions: Estimation of expression levels of microRNAs in cellular aspirate of STN is feasible and accurate for detection of the malignancy. Down-expression of miR-203a and over-expression of miR-576 in tissue aspirate are more diagnostic for TC and can specify PTC cases out of other TC cases.

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Section: Discussionmentioning

confidence: 96%

The diagnostic performance of microRNAs expression levels in cellular aspirate of solitary thyroid nodule overrides that of cytology

Hussein,

Hafez,

Marie

et al. 2023

Int Surg J

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“…Concurrently, Zhang et al study results MALAT1/miR-203a/ TGβR2 has a significant ceRNET in PCOS. The utility of miR-203a-3p as a valuable diagnostic and prognostic biomarker for the spectrum of thyroid neoplasia [ 164 ]. Extensive research has confirmed the significant role of miR-203 and its diverse mature miRNAs, including miR-203a and miR-203b, as tumor suppressors in the pathogenesis and advancement of human malignancies [ 165 , 166 ].…”

Section: Proven and Potential Study Of Novel Cernets In Pcosmentioning

confidence: 99%

Biomarker potential of competing endogenous RNA networks in Polycystic Ovary Syndrome (PCOS)

Heidarzadehpilehrood,

Pirhoushiaran

2024

Non-coding RNA Research

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“…Reverse transcription and quantitative real-time PCR for miR-203a-3p and miR-204-3p are described in detail in our previously published paper [22]. Table 1 provides information about the sequences of stem-loop primers used in reverse transcription reactions, as well as the sequences of the primers used in qPCR.…”

Section: Reverse Transcription and Quantitative Real-time Pcr For Mir...mentioning

confidence: 99%

“…MicroRNAs (miRs) are a subgroup of short ncRNAs (19)(20)(21)(22)(23) nucleotides in length) that control the expression of genes in various biological processes, such as cell differentiation, proliferation and apoptosis. Due to its suppressive effect, a certain regulatory mechanism involving miR may lead to the downregulation of tumor suppressor genes and/or the upregulation of oncogenes [17].…”

Section: Introductionmentioning

confidence: 99%

“…MiR-203a-3p and miR-204-3p are newly discovered miRs with deregulated expression in PTC and with a rather unknown biological function. Studies have shown that miR-203a-3p and miR-204-3p are often downregulated in thyroid cancer, which can lead to increased expression of oncogenes and decreased expression of tumor suppressor genes [20][21][22]. Furthermore, a study on colorectal cancer cells found that BANCR works as a miR-203 sponge [23], while a study on melanoma cells discovered that miR-204 is a direct target of BANCR [24].…”

Section: Introductionmentioning

confidence: 99%

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BRAFV600E, BANCR, miR-203a-3p and miR-204-3p in Risk Stratification of PTC Patients

Stojanović,

Šelemetjev,

Đorić

et al. 2023

Biomedicines

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In order to enhance the risk stratification of papillary thyroid carcinoma (PTC) patients, we assessed the presence of the most common mutation in PTC (BRAFV600E) with the expression profiles of long non-coding RNA activated by BRAFV600E (BANCR) and microRNAs, which share complementarity with BANCR (miR-203a-3p and miR-204-3p), and thereafter correlated it with several clinicopathological features of PTC. BRAFV600E was detected by mutant allele-specific PCR amplification. BANCR and miRs levels were determined by quantitative RT-PCR. Bioinformatic analysis was applied to determine the miRs’ targets. The expression profile of miR-203a-3p/204-3p in PTC was not affected by BRAFV600E. In the BRAFV600E-positive PTC, high expression of miR-203a-3p correlated with extrathyroidal invasion (Ei), but the patients with both high miR-203a-3p and upregulated BANCR were not at risk of Ei. In the BRAFV600E-negative PTC, low expression of miR-204-3p correlated with Ei, intraglandular dissemination and pT status (p < 0.05), and the mutual presence of low miR-204-3p and upregulated BANCR increased the occurrence of Ei. Bioinformatic analysis predicted complementary binding between miR-203a-3p/204-3p and BANCR. The co-occurrence of tested factors might influence the spreading of PTC. These findings partially describe the complicated network of interactions that may occur during the development of PTC aggressiveness, potentially providing a new approach for high-risk PTC patient selection.

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MiR-203a-3p, miR-204-3p, miR-222-3p as useful diagnostic and prognostic tool for thyroid neoplasia spectrum

Cited by 3 publications

References 39 publications

The diagnostic performance of microRNAs expression levels in cellular aspirate of solitary thyroid nodule overrides that of cytology

The diagnostic performance of microRNAs expression levels in cellular aspirate of solitary thyroid nodule overrides that of cytology

Biomarker potential of competing endogenous RNA networks in Polycystic Ovary Syndrome (PCOS)

BRAFV600E, BANCR, miR-203a-3p and miR-204-3p in Risk Stratification of PTC Patients

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