MiR‐202 inhibits cell proliferation, invasion, and migration in breast cancer by targeting ROCK1 gene

Xu, Fangfang; Li, Hui; Hu, Chengjiu

doi:10.1002/jcb.28879

Cited by 22 publications

(15 citation statements)

References 41 publications

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“…In previous studies, miR-202 also was found to be aberrantly expressed in endometrial tissues from patients with EMS ( 13 , 38 ). In addition, miR-202 has been previous shown to exert suppressive roles on the invasive and migratory capacities of tumor cells ( 43 - 45 ). According to the aforementioned findings from the present and previous studies, it could be hypothesized that miR-202 can regulate EMS progression.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that miR-202 mediates suppressive invasive roles in various types of human tumors ( 21 , 44 ). Xu et al ( 45 ) reported that miR-202 targeted Rho-associated protein kinase 1 to inhibit the invasive and migratory abilities of breast cancer. In addition, Zhang et al ( 18 ) found that miR-202 upregulation resulted in significant reductions in the migration and invasion of prostate cancer cells by targeting the phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway

et al. 2020

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The enhanced migratory ability of endometrial stromal cells (ESCs) is a key factor in the formation of functional endometrium-like tissues outside the uterine cavity during endometriosis (EMS). Although accumulating evidence has suggested the importance of microRNAs (miRNAs) in the pathogenesis of EMS, the role of particular miRNAs in the invasiveness of ESCs remain poorly understood. In the present study, the function of miRNAs in the invasiveness of ESCs, along with the associated underlying mechanism involved, were investigated. Initially, the expression patterns of miRNAs in the ectopic and eutopic endometrium isolated from patients with EMS were analyzed using microarray. MicroRNA-202-5p (miR-202) was selected for further study due to its previously reported suppressive effects on the invasion in various types of cancers. The expression of miR-202 and K-Ras in eutopic and ectopic endometrioma tissues were detected using reverse transcription-quantitative PCR, immunohistochemistry and western blotting. The migration and invasion ability of ESCs was determined using wound healing and Transwell invasion assays, respectively. Compared with that from healthy individuals, miR-202 expression was demonstrated to be lower in the eutopic endometrium from patients with EMS, which was even lower in ectopic endometrium. Functional experiments in primary ESCs revealed that enhanced miR-202 expression suppressed the cell invasion and migration abilities, which was also accompanied with increased E-cadherin and reduced N-cadherin expression in ESCs, suggesting its potentially suppressive role in epithelial-mesenchymal transition. K-Ras is a well-known regulator of the ERK signaling pathway that was shown to be directly targeted and negatively regulated by miR-202. In addition, K-Ras expression was found to be upregulated in the ectopic endometrium, where it correlated negatively with that of miR-202. Knocking down K-Ras expression mimicked the anti-invasive effects of miR-202 overexpression on ESCs, whilst K-Ras overexpression attenuated the inhibitory role of miR-202 overexpression in ESC invasion. The K-Ras/Raf1/MEK/ERK signaling pathway was also blocked by miR-202 overexpression. These findings suggested that miR-202 inhibited ESC migration and invasion by inhibiting the K-Ras/Raf1/MEK/ERK signaling pathway, rendering miR-202 a candidate for being a therapeutic target for EMS.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway

et al. 2020

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“…Recent studies have demonstrated that miR-202 is associated with several types of cancers such as ovarian cancer [12], lung cancer [13], and colorectal cancer [14]. miR-202 has been reported to have either a tumor-suppressive or oncogenic function (Table 1) [15][16][17][18][19][20][21][22][23][24][25][26][27][28]. Breast cancer blood up metastasis, poor survival outcome - [24] This discrepancy is presumed to be the result of differences in the sample processing methods, type of samples, detection methods, and characteristics of the recruited study groups.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, it was highly expressed in the drug-resistant breast cancer tissues [25]. However, some studies reported that it was downregulated in breast cancer cells and inhibited the tumorigenesis of breast cancer [27,28]. Although miR-202 has been studied for its function or expression in breast cancer, little is known about its potential diagnostic value in a clinical setting.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of Circulating miR-202 in Early-Stage Breast Cancer in South Korea

et al. 2020

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Background and objectives: Breast cancer is the most common cancer among women worldwide. Early stage diagnosis is important for predicting increases in treatment success rates and decreases in patient mortality. Recently, circulating biomarkers such as circulating tumor cells, circulating tumor DNA, exosomes, and circulating microRNAs have been examined as blood-based markers for the diagnosis of breast cancer. Although miR-202 has been studied for its function or expression in breast cancer, its potential diagnostic value in a clinical setting remains elusive and miR-202 has not been investigated in South Korea. In this study, we aimed to evaluate the diagnostic utility of miR-202 in plasma samples of breast cancer patients in South Korea. Materials and Methods: We investigated miR-202 expression in the plasma of 30 breast cancer patients during diagnosis along with 30 healthy controls in South Korea by quantitative reverse transcription PCR. Results: The results showed that circulating miR-202 levels were significantly elevated in the breast cancer patients compared with those in healthy controls (p < 0.001). The sensitivity and specificity of circulating miR-202 were 90.0% and 93.0%, respectively. Additionally, circulating miR-202 showed high positivity at early stage. The positive rate of miR-202 was as follows: 100% (10/10) for stage I, 90% (9/10) for stage II, and 80% (8/10) for stage III. miR-202 was also a predictor of a 9.6-fold high risk for breast cancer (p < 0.001). Conclusions: Additional alternative molecular biomarkers for diagnosis and management of pre-cancer patients are needed. Circulating miR-202 might be potential diagnostic tool for detecting early stage breast cancer.

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“…The particular miRNAs in this paper are associated with cancer occurrence, metastasis and invasion. Indeed, miR-145, miR-202 and miR-374 has been proposed as diagnostic, prognostic or therapeutic marker of BLCA [45,46] .miR-202 and miR-347 participate in invasion, metastasis and cancer progression [46,47], while miR-145 modulates suppressor of cytokine signaling 7 (socs7) to enhance IFN-β expression, thus contributing to BLCA apoptosis. We speculate that deregulation of these miRNAs would be in consistence with the phenotype of POLR2K overexpression in BLCA, which should be further veri ed by experiments.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression and Regulatory Webwork of POLR2K in Bladder Carcinogenesis by Integrated Bioinformatics Approaches

Yang

Wang

Guo

et al. 2020

Preprint

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Background：RNA polymerase II subunit K (POLR2K) belongs to one of the multiple subunits of RNA polymerase II (Pol II), whose biological function is to synthesize mRNA. Aberrant POLR2K expression is related to carcinogenesis. However, POLR2K’s underlying role in bladder cancer has not been explored. In the current study, we intend to analyze the function of POLR2K and its regulatory network within bladder cancer.Methods: Public sequencing data was obtained from GEO and TCGA to investigate POLR2K expression and regulatory network within bladder cancer (BLCA) by using GEPIA and Oncomine as well as cBioPortal online tool. LinkedOmics was employed to identify genes displaying significantly differential expression patterns and to perform GO and KEGG analyses. After differential genes was assigned and ranked, GSEA analyses was performed to obtain target networks for transcription factors, miRNAs, and kinases that could regulate POLR2K–associated gene network. Subsequent functional webwork analyses were used to identify cancer-relevant pathways Moreover, POLR2K gene is verified, by ChIP-seq in MCF-7 cell line , with transcription factor binding evidence in the ENCODE Transcription Factor Binding Site Profiles dataset.Conclusions: The current study implies that POLR2K gene is overexpressed and often amplified in BLCA, providing the first evidence that POLR2K deregulation, in particular increased transcription, may promote BLCA. These findings uncover a unique expression patterns of POLR2K and its potential regulatory networks in BLCA, contributing greatly to study of the role of POLR2K in cancer development.

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MiR‐202 inhibits cell proliferation, invasion, and migration in breast cancer by targeting ROCK1 gene

Cited by 22 publications

References 41 publications

MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway

MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway

Diagnostic Value of Circulating miR-202 in Early-Stage Breast Cancer in South Korea

Gene Expression and Regulatory Webwork of POLR2K in Bladder Carcinogenesis by Integrated Bioinformatics Approaches

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