miR-19b promotes breast cancer metastasis through targeting MYLIP and its related cell adhesion molecules

Zhao, Luqing; Zhao, Yue; He, Ye; Mao, Yun

doi:10.18632/oncotarget.19278

Cited by 41 publications

(21 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 29 In breast cancer, the expression of miR-19b has also been investigated and was demonstrated to be elevated in tumor tissues compared with the normal controls. 30 Similarly, the results of our research revealed that miR-19b expression, measured by qRT-PCR, was markedly higher in breast cancer tissues than in the matched normal tissues. The expression results in breast cancer cells were inconsistent with the tissues, which also showed increased miR-19b expression levels in cancer cell lines compared with the normal cells.…”

Section: Discussionsupporting

confidence: 73%

miR-19b serves as a prognostic biomarker of breast cancer and promotes tumor progression through PI3K/AKT signaling pathway

Li¹,

Zhang²,

Zhang³

et al. 2018

OTT

View full text Add to dashboard Cite

BackgroundMicroRNAs (miRNAs) are involved in tumor progression of various human malignancies. MicroRNA-19b (miR-19b) has been described as serving a crucial role in tumorigenesis of breast cancer. The purpose of this study was to investigate the expression patterns, clinical value, and functional role of miR-19b in breast cancer.MethodsExpression of miR-19b was estimated by quantitative real time PCR. Kaplan–Meier survival analysis and Cox regression assay were performed to explore the prognostic value of miR-19b. The functional role of miR-19b was verified using cell experiments.ResultsUpregulated miR-19b expression was observed in breast cancer tissues and cells compared with the controls (all P<0.05). The miR-19b expression was associated with distant metastasis and TNM stage. The survival curves showed that high miR-19b was correlated with poor overall survival of the patients (log-rank P=0.002). Furthermore, miR-19b was proven to be an independent prognostic factor for patients. By using miR-19b mimic and inhibitor, cell proliferation, migration, and invasion were enhanced by miR-19b overexpression but were suppressed by reduction of miR-19b (all P<0.05). Besides, PI3K/AKT was demonstrated to be activated by miR-19b in breast cancer cells.ConclusionThe overexpression of miR-19b serves as a candidate prognostic biomarker of breast cancer and may be involved in the tumor progression through PI3K/AKT pathway.

show abstract

Section: Discussionsupporting

confidence: 73%

miR-19b serves as a prognostic biomarker of breast cancer and promotes tumor progression through PI3K/AKT signaling pathway

Li¹,

Zhang²,

Zhang³

et al. 2018

OTT

View full text Add to dashboard Cite

show abstract

“…Myosin-regulated light chain interacting protein (MYLIP), also known as ubiquitin-protein ligase, regulates the post-transcriptional diversity of low-density lipoprotein (LDL) receptors. Studies have confirmed that MYLIP inhibits the metastasis and progression of breast cancer [35]. Our study indicated that MYLIP was a tumor suppressor during the progression of LUAD, and hypoxia promoted DNA methylation of MYLIP.…”

Section: Discussionsupporting

confidence: 68%

Genome-wide analysis of the hypoxia-related DNA methylation-driven genes in lung adenocarcinoma progression

Hong

et al. 2020

Bioscience Reports

View full text Add to dashboard Cite

Lung adenocarcinoma (LUAD) is a common type of lung cancer with high incidence and poor prognosis. Hypoxia and DNA methylation play important regulatory roles in cancer progression. The purpose of the present study was to explore the relationship between hypoxia and DNA methylation, and to identify key genes for hypoxia-regulated LUAD progression. Hypoxia score (HS) was calculated using the GSVA algorithm. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and protein–protein interaction (PPI) analysis were performed using clusterProfile package, STRING database and Cytoscape software. Kaplan–Meier curves of overall survival (OS) and disease-free survival (DFS) were drawn using R software. Smoking status and cancer stages were significantly associated with LUAD hypoxia, and hypoxia is a poor prognostic factor for LUAD. Compared with HS-low group, 1803 aberrantly methylated DEGs were identified in HS-high group. KEGG analysis showed that the 1803 genes were enriched in the metabolic pathways associated with hypoxia stress, angiogenesis and cancer progression. FAM20C, MYLIP and COL7A1 were identified as the hypoxia-related key genes in LUAD progression, which were regulated by DNA methylation. Hypoxia in LUAD tumor cells led to changes in DNA methylation patterns. In-depth study of the relationship between hypoxia and DNA methylation is helpful to elucidate the mechanism of tumorigenesis, and provides new ideas for LUAD treatment.

show abstract

“…Compared with the untreated cells, miR-19b was significantly downregulated in SGC-7901 cells following matrine treatment. Another study reported that miR-19b promoted breast cancer metastasis by targeting myosin regulatory light chain interacting protein and associated cell adhesion molecules (19). Additionally, it has been reported that miR-19b-3p may promote colon cancer proliferation and oxaliplatin-based chemoresistance by targeting SMAD4 (20).…”

Section: Introductionmentioning

confidence: 99%

Matrine exerts inhibitory effects in melanoma through the regulation of miR-19b-3p/PTEN

et al. 2018

View full text Add to dashboard Cite

Matrine, one of the main alkaloid components extracted from the traditional Chinese herb, Sophora flavescens Ait, has various pharmacological effects, and has been reported to exert antitumor activity in melanoma. In the current study, the molecular mechanisms underlying the inhibitory effects of matrine were investigated in melanoma cell line. It was initially confirmed that matrine inhibited proliferation, invasion and induced apoptosis in human A375 and SK-MEL-2 melanoma cell lines in vitro. Subsequently, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that the expression of microRNA (miR)-19b-3p was significantly increased in melanoma cells and was downregulated by treatment with matrine. Furthermore, downregulated miR-19b-3p exerted effects similar to 500 µg/ml matrine on cell proliferation, invasion and apoptosis. Phosphatase and tensin homolog (PTEN) mRNA was identified as a direct target of miR-19b-3p through bioinformatics analysis and a dual-luciferase reporter assay. Additionally, western blotting and RT-qPCR analysis demonstrated that the expression of PTEN protein and mRNA were increased by the treatment with matrine. Furthermore, silencing of PTEN expression reversed the effects of matrine and miR-19b-3p downregulation in A375 and SK-MEL-2 cells. Taken together, the results indicated that matrine may suppress cell proliferation and invasion and induce cell apoptosis partially via miR-19b-3p targeting of PTEN.

show abstract

miR-19b promotes breast cancer metastasis through targeting MYLIP and its related cell adhesion molecules

Cited by 41 publications

References 36 publications

miR-19b serves as a prognostic biomarker of breast cancer and promotes tumor progression through PI3K/AKT signaling pathway

miR-19b serves as a prognostic biomarker of breast cancer and promotes tumor progression through PI3K/AKT signaling pathway

Genome-wide analysis of the hypoxia-related DNA methylation-driven genes in lung adenocarcinoma progression

Matrine exerts inhibitory effects in melanoma through the regulation of miR-19b-3p/PTEN

Contact Info

Product

Resources

About