miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma

Abstract

Introduction. miR-199a has been reported as an oncogene of various cancers. However, the biological function and regulatory mechanism of miR-199a in keratinocytes of cholesteatoma are still unclear. Methods. Detection by qRT-PCR was conducted on miR-199a’s expression in both thirty pairs of cholesteatoma tissues and normal skins. For characterizing the function of miR-199a, this research adopted transwell assay, wound healing assay, and CCK8 assays. Under the support of qRT-PCR, efforts were made to investigate the relative expression of candidate target genes. Moreover, the evaluation of the targeting relationship between miR-199a and the candidate target gene was conducted with the dual-luciferase reporter assay. Results. The upregulation of miR-199a was found in cholesteatoma tissues, which facilitated the proliferation, migration, and invasion of HaCaT cells, while its downregulation caused opposite results. Conclusions. The findings of the present research offer more insights into the molecular mechanism of cholesteatoma progression.

Expression and Regulatory Mechanisms of MicroRNA in Cholesteatoma: A Systematic Review

“…In total, 118 articles were retrieved through the database search. After applying the search strategy described above, a total of 18 articles were identified that met all inclusion criteria [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47]. A flow diagram of the detailed study selection process is presented in Figure 1.…”

“…All included studies used cholesteatoma tissues for analysis. Additionally, the majority of studies included in vitro experiments on cell lines [30,32,[34][35][36][37][38][39][42][43][44]46,47]. In the Zheng study [46], a mouse model was also used for in vivo testing.…”

“…Sui, R. [39] miR-142-5p downregulated Yao, L. [42] miR-199a upregulated Liu, D. [38] miR-508-3p upregulated Hu, Y. [35] miR-22-3p, miR-125a-5p upregulated Gong, N. [34] exosomal miR-17 downregulated Zang, J.…”

Micro RNAs and Circular RNAs in Different Forms of Otitis Media

“…Yao et al analyzed the role of microRNA-199a (miR-199a), a cancer-promoting microRNA, as a probable factor influencing the development of cholesteatoma [ 34 ]. They revealed that miR-199a is upregulated in cholesteatoma tissues, which is thought to promote keratinocyte proliferation, migration, and invasion in this pathology.…”

“…They revealed that miR-199a is upregulated in cholesteatoma tissues, which is thought to promote keratinocyte proliferation, migration, and invasion in this pathology. PNRC1 was found to be a direct target of miR-199a, with a negative regulatory interrelationship [ 34 ].…”