miR‑197 is downregulated in cervical carcinogenesis and suppresses cell proliferation and invasion through targeting forkhead box M1

Abstract

Abstract. Cervical cancer is the second most common type of cancer in females worldwide. It has been demonstrated that microRNAs (miRs) serve important roles in the occurrence and development of various types of cancer, including cervical cancer. The results of the present study revealed that miR-197 was downregulated in cervical cancer tissues and cell lines. Restoration of miR-197 expression significantly inhibited cell viability and invasion of cervical cancer. Additionally, forkhead box M1 (FOXM1) was identified as a direct target gene of miR-197. Bioinformatic analysis revealed that FOXM1 was a potential target gene of miR-197. Luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction and western blot analysis demonstrated that miR-197 decreased FOXM1 expression through direct binding to its 3'-untranslated region. Furthermore, the effects of FOXM1 underexpression were comparable with the effects induced by miR-197 overexpression in cervical cancer cells, suggesting that FOXM1 acted as a downstream effector in miR-197-mediated proliferation and invasion of cervical cancer cells. The results of the present study suggested that miR-197 inhibited growth and metastasis of cervical cancer by directly targeting FOXM1.

miR‐197‐5p inhibits sarcomagenesis and induces cellular senescence via repression of KIAA0101

“…Further, miR‐197 has garnered a lot of attention for its explicit role in the development and metastasis of tumors playing as a double‐edged sword, either acting as a tumor suppressive or oncogenic miRNA in different cancer subtypes . The tumorigenic role of miR‐197 is evident from its crucial contributions in cell differentiation, cell growth, apoptosis, metastasis, angiogenesis, drug resistance, as well as various other cellular processes via targeting key tumor‐suppressive or oncogenic genes such as NOXA, BMF, and FUS1 .…”

MicroRNA‑876‑5p inhibits the progression of glioblastoma multiforme by directly targeting Forkhead box�M1

“…Considerable evidence supports the importance of FOXM1 in the GBM genesis and development through regulation of multiple biological behaviours, including cell proliferation, cell cycle, apoptosis, metastasis, epithelial to mesenchymal transition, chemosensitivity and angiogenesis (30)(31)(32)(33)(34). Previously, accumulating studies reported that FOXM1 may be regulated by multiple miRNAs in various cancers, such as miR-197 in cervical cancer (38), miR-761 in colorectal cancer (25), miR-320 in glioma, and miR-630 in gastric cancer (39). In the present study, for the first time to the best of the authors knowledge, it was demonstrated that FOXM1 was negatively regulated by miR-876-5p in GBM, and therefore inhibited the progression and development of GBM.…”

<p>Downregulation of circRNA_0000285 Suppresses Cervical Cancer Development by Regulating miR197-3p–ELK1 Axis</p>

“…In this research, downregulated miR197-3p was measured in CC, consistent with former work. 18 That paper showed that miR197-3p had a tumor-suppressive role in CC by suppressing proliferation and invasion. 18 Similarly, our research also identified the antitumor effect of miR197-3p in CC via regulating cell viability, colony formation, the cell cycle, apoptosis, and autophagy.…”

“… 16 , 17 Moreover, miR197-3p can suppress proliferation and invasion of CC cells. 18 However, whether miR197-3p is required for circ_0000285 -mediated regulation of CC development is unclear. Furthermore, ELK1 has been suggested to have a carcinogenic role in human cancers, like breast cancer, thyroid cancer, hepatocellular carcinoma, and colorectal cancer.…”