miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children

Visani, Michela; Marucci, Gianluca; Biase, Dario de; Giangaspero, Felice; Buttarelli, Francesca Romana; Brandes, Alba Ariela; Franceschi, Enrico; Acquaviva, Giorgia; Ciarrocchi, Alessia; Rhoden, Kerry J.; Tallini, Giovanni; Pession, Annalisa

doi:10.3390/diagnostics10050265

Cited by 7 publications

(3 citation statements)

References 109 publications

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“…Zhang et al showed that miR-200b-3p was also upregulated in neonatal rat brains in the early stage following hypoxia-ischemic injury, and that its inhibition led to a diminished neuronal apoptotic rate [106]. Medulloblastomas exhibit differential expression of miR-200b-3p according to age, with adults presenting an overexpression compared to pediatric patients [107]. Furthermore, miR-200b-3p among other miRNAs was upregulated in patients with brain metastatic gastric adenocarcinomas when compared to patients with this cancer but without cerebral metastases [108].…”

Section: Mir-200b-3pmentioning

confidence: 99%

An Insight into the microRNAs Associated with Arteriovenous and Cavernous Malformations of the Brain

Florian

Buruiană

Şuşman

et al. 2021

Cells

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Background: Brain arteriovenous malformations (BAVMs) and cerebral cavernous malformations (CCMs) are rare developmental anomalies of the intracranial vasculature, with an irregular tendency to rupture, and as of yet incompletely deciphered pathophysiology. Because of their variety in location, morphology, and size, as well as unpredictable natural history, they represent a management challenge. MicroRNAs (miRNAs) are strands of non-coding RNA of around 20 nucleotides that are able to modulate the expression of target genes by binding completely or partially to their respective complementary sequences. Recent breakthroughs have been made on elucidating their contribution to BAVM and CCM occurrence, growth, and evolution; however, there are still countless gaps in our understanding of the mechanisms involved. Methods: We have searched the Medline (PubMed; PubMed Central) database for pertinent articles on miRNAs and their putative implications in BAVMs and CCMs. To this purpose, we employed various permutations of the terms and idioms: ‘arteriovenous malformation’, ‘AVM’, and ‘BAVM’, or ‘cavernous malformation’, ‘cavernoma’, and ‘cavernous angioma’ on the one hand; and ‘microRNA’, ‘miRNA’, and ‘miR’ on the other. Using cross-reference search; we then investigated additional articles concerning the individual miRNAs identified in other cerebral diseases. Results: Seven miRNAs were discovered to play a role in BAVMs, three of which were downregulated (miR-18a, miR-137, and miR-195*) and four upregulated (miR-7-5p, miR-199a-5p, miR-200b-3p, and let-7b-3p). Similarly, eight miRNAs were identified in CCM in humans and experimental animal models, two being upregulated (miR-27a and mmu-miR-3472a), and six downregulated (miR-125a, miR-361-5p, miR-370-3p, miR-181a-2-3p, miR-95-3p, and let-7b-3p). Conclusions: The following literature review endeavored to address the recent discoveries related to the various implications of miRNAs in the formation and growth of BAVMs and CCMs. Additionally, by presenting other cerebral pathologies correlated with these miRNAs, it aimed to emphasize the potential directions of upcoming research and biological therapies.

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Section: Mir-200b-3pmentioning

confidence: 99%

An Insight into the microRNAs Associated with Arteriovenous and Cavernous Malformations of the Brain

Florian

Buruiană

Şuşman

et al. 2021

Cells

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“…These differentially expressed miRNAs were expected to be key indicators for the identification of specific MB-associated markers. Visani et al (35) indicated that there were differences in miR-196B-5P and miR-200B-3P expression between adults and children. Their study suggested that miRNAs may be involved in the development of MB, but not in the differences noted in the biological responses of adults and children with MB.…”

Section: Mirnas In Mbmentioning

confidence: 99%

The mechanism of non‑coding RNAs in medulloblastoma (Review)

Zhao

Han

et al. 2021

Oncol Lett

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Medulloblastoma (MB) is one of the most common malignant tumors of the central nervous system in children. Although surgery, radiotherapy and chemotherapy have resulted in considerable progress in the treatment of this disease, the prognosis of patients with MB remains very poor. Therefore, highly specific molecular targeted treatment, which can improve the therapeutic efficacy and reduce the side effects of MB, has become a research hotspot. In recent years, non-coding RNAs (ncRNAs), which were initially considered to be transcriptional noise, have been shown to possess regulatory functions. A series of ncRNAs have been identified, including microRNAs and circular RNAs, which affect the expression of specific genes in a variety of tumors. These genes lead to the formation of a specific complex of proteins or they directly participate in protein synthesis in order to regulate the occurrence and development of tumors. The aim of the present review article was to summarize the recent research studies that have explored the ability of ncRNAs to regulate the occurrence and development of MB. Contents 1. Introduction 2. miRNAs in MB 3. circRNAs in MB 4. Other ncRNAs in MB 5. Conclusions

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“…CRK and CRK-like, 2 cellular counterparts of viral oncogene v-Crk, are critically implicated in the tumor-forming process of tumor cells. 12 A recent study has identified CRK as an oncogene in Wilm’s tumor by enhancing cell invasion, migration and inducing mesenchymal-to-epithelial transition. 13 In light of those findings, we speculated that miR-215-5p might target CRK to affect the biological behaviors of cancer cells in Wilm’s tumor.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-215-5p Inhibits the Proliferation and Migration of Wilm’s Tumor Cells by Targeting CRK

Dang

et al. 2021

Technol Cancer Res Treat

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Objective: Wilm’s tumor is a common renal malignancy in childhood with unsatisfactory prognosis. microRNA-215-5p (miR-215-5p) has been reported as a tumor-suppressive miRNA in different types of human cancers, but rarely in the Wilm’s tumor. In light of this, we tried to investigate the regulatory role and underlying mechanism of miR-215-5p in the Wilm’s tumor. Methods: After sample collection and cell culture, the expression of miR-215-5p and CT10 Regulator of Kinase (CRK) was detected. Then rhabdoid tumor cell lines (formerly classified as Wilms’ tumor cell lines), G401 and WT-CLS1 cells were transfected with pcDNA3.1, pcDNA3.1-CRK, sh-NC, sh-CRK, agomir NC, miR-215-5p agomir, antagomir NC or miR-215-5p antagomir to explore the function of miR-215-5p and CRK in the Wilm’s tumor cell proliferation and migration. Moreover, the relationship between miR-215-5p and CRK was analyzed by dual luciferase reporter gene assay. Results: Lowly-expressed miR-215-5p and highly-expressed CRK were observed in the Wilm’s tumor tissues and cells. Transfection of pcDNA3.1-CRK or miR-215-5p antagomir could promote G401 and WT-CLS1 cell proliferation and enhance migration ability, while transfection of sh-CRK or miR-215-5p agomir led to opposite results. Additionally, miR-215-5p may bind to CRK. Moreover, transfection of pcDNA3.1-CRK in G401 and WT-CLS1 cells could partially reverse the inhibitory effect of miR-215-5p agomir on the proliferation and migration of Wilm’s tumor cells. Conclusion: Our study highlighted that miR-215-5p could suppress the proliferation and migration of Wilm’s tumor cells by regulating the expression of CRK, providing new ideas for molecular targeted therapy for Wilm’s tumor.

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miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children

Cited by 7 publications

References 109 publications

An Insight into the microRNAs Associated with Arteriovenous and Cavernous Malformations of the Brain

An Insight into the microRNAs Associated with Arteriovenous and Cavernous Malformations of the Brain

The mechanism of non‑coding RNAs in medulloblastoma (Review)

MicroRNA-215-5p Inhibits the Proliferation and Migration of Wilm’s Tumor Cells by Targeting CRK

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