miR-193b and miR-30c-1* inhibit, whereas miR-576-5p enhances melanoma cell invasion in vitro

Abstract

In cancer cells, microRNAs (miRNAs) are often aberrantly expressed resulting in impaired mRNA translation. In this study we show that miR-193b and miR-30c-1* inhibit, whereas miR-576-5p accelerates invasion of various human melanoma cell lines. Using Boyden chamber invasion assays the effect of selected miRNAs on the invasive capacity of various human melanoma cell lines was analyzed. Upon gene expression profiling performed on transfected A375 cells, CTGF, THBS1, STMN1, BCL9, RAC1 and MCL1 were identified as potential targets. For target validation, qPCR, Western blot analyses or luciferase reporter assays were applied. This study reveals opposed effects of miR-193b / miR-30c-1* and miR-576-5p, respectively, on melanoma cell invasion and on expression of BCL9 and MCL1, possibly accounting for the contrasting invasive phenotypes observed in A375 cells transfected with these miRNAs. The miRNAs studied and their targets identified fit well into a model proposed by us explaining the regulation of invasion associated genes and the observed opposed phenotypes as a result of networked direct and indirect miRNA / target interactions. The results of this study suggest miR-193b and miR-30c-1* as tumor-suppressive miRNAs, whereas miR-576-5p appears as potential tumor-promoting oncomiR. Thus, miR-193b and miR-30c-1* mimics as well as antagomiRs directed against miR-576-5p might become useful tools in future therapy approaches against advanced melanoma.

miR‐197‐5p inhibits sarcomagenesis and induces cellular senescence via repression of KIAA0101

“…It is a well‐accepted fact that the aberrant expression of miRNAs play significant roles in the carcinogenesis and progression of human cancers . Therefore, in‐depth understanding of the roles of miRNAs shall provide new dimension into molecular mechanism involved in the tumor formation and growth of cancer as well for the development of novel therapeutic strategies .…”

WITHDRAWN: A Novel Long Non-coding RNA, ENSG00000236199, Inhibits Proliferation and Metastasis through NRIP1 by Sponging miR-576-5p in Hepatocellular Carcinoma

“…In the past, miRNAs were also considered as non-functional RNA, but more and more studies have shown that miRNAs are involved in various biological functions of cells (Tarallo et al 2019;Xu et al 2020;Vignard et al 2020). Many studies have shown that miRNA-576-5p plays an important role in a variety of cancers, such as esophageal cancer (Zhang et al 2019), colorectal cancer (Makondi et al 2019) and melanoma (Kordaß et al 2018).…”

miR‑576‑5p promotes epithelial‑to‑mesenchymal transition in colorectal cancer by targeting the Wnt5a‑mediated Wnt/β‑catenin signaling pathway

“…In addition, miRNAs have been reported to serve as effective biomarkers for the early diagnosis and prognosis of patients with cancer ( 10 ). A previous study demonstrated that miR-576-5p might be a potential tumor-promoting oncomiR in several types of human tumor ( 11 ). Kordass et al ( 11 ) reported that miR-576-5p enhances the invasion of melanoma cells in vitro .…”

“…A previous study demonstrated that miR-576-5p might be a potential tumor-promoting oncomiR in several types of human tumor ( 11 ). Kordass et al ( 11 ) reported that miR-576-5p enhances the invasion of melanoma cells in vitro . In addition, miR-576-5p was also found to be upregulated in esophageal cancer, where it was found to be involved in cancer cell migration and invasion, suggesting that it might serve as a predictor of cancer prognosis ( 12 , 13 ).…”