miR-193 expression differentiates telocytes from other stromal cells

Cismasiu, Valeriu B.; Radu, Eugen; Popescu, Laurențiu M.

doi:10.1111/j.1582-4934.2011.01325.x

Cited by 108 publications

(88 citation statements)

References 25 publications

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“…These cells are markedly different from TC, but some of the cells labeled as 'fibroblasts' in the figures reported in histology textbooks and other literature show the morphology of TC and not that of active fibroblasts. Recently, some markers have been tested to differentiate cardiac TC from fibroblasts (which are c-kit negative) (39). Noteworthy, microRNA expression (e.g., miR-193) clearly differentiated TC from fibroblasts and in culture also allowed to discriminate between TC and other stromal cells (39).…”

Section: Possible Tc Identification With 'Already Identified' Stromalmentioning

confidence: 99%

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Telocytes

Pellegrini

Popescu²

2011

BioMolecular Concepts

145

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Here, we review the history, morphology, immunohistochemical phenotype, and presumptive roles of a new type of interstitial tissue cells, formerly called interstitial Cajal-like cells (ICLC) and by 2010 named 'telocytes' (TC). Many different techniques have been used to characterize TC and provide their unequivocal identification: (i) in vitro, cultures and isolated cells; (ii) in situ, fixed specimens examined by light and fluorescence microscopy, transmission (TEM) and scanning electron microscopy, and electron tomography. TEM allowed sure identification and characterization of the most peculiar feature of TC: the long, thin, and convoluted prolongations named 'telopodes'. An enormous variety of antibodies have been tested, but presently none are reliable to specifically label TC. TC have a mesenchymal origin and are resident connective tissue (stromal) cells. Possible identification with 'already identified' stromal cell types (fibroblasts, fibrocytes, fibroblast-like cells, and mesenchymal stromal cells) is discussed. We conclude that in adulthood, most of the TC have the morphology of fibrocytes. Apparently, immunocytochemistry suggests that a variety of TC populations showing different, likely organ-specific, immunophenotypes might exist. Several roles have been hypothesized for TC: mechanical roles, intercellular signaling, guiding and nursing of immature cells during organogenesis, and being themselves a pool of precursors for many of the mesenchyme-derived cells in adulthood; however, none of these roles have been proven yet. On the basis of the available data, we propose TC may be key players in organ regeneration and repair.

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Section: Possible Tc Identification With 'Already Identified' Stromalmentioning

confidence: 99%

“…Recently, some markers have been tested to differentiate cardiac TC from fibroblasts (which are c-kit negative) (39). Noteworthy, microRNA expression (e.g., miR-193) clearly differentiated TC from fibroblasts and in culture also allowed to discriminate between TC and other stromal cells (39).…”

Section: Possible Tc Identification With 'Already Identified' Stromalmentioning

confidence: 99%

Telocytes

Pellegrini

Popescu²

2011

BioMolecular Concepts

145

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“…There is evidence that renal telocytes co-express CD34, vimentin, and CD117 [16]. Furthermore, previous studies also demonstrated an obvious difference between telocytes and other cell populations, especially fibroblasts, in their microRNA imprint, proteome genetic profiles, and secretome levels [19-21]. These results indicate that telocytes are a unique cell population.…”

Section: Introductionmentioning

confidence: 59%

Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats

Zheng

et al. 2018

Cell Physiol Biochem

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Background/Aims: Previous studies imply that telocytes may have a protective effect on fibrosis in various organs, including the liver, colon, and heart. The effect of telocytes on renal fibrosis remains unknown. Herein, this study was designed to investigate the effect of telocytes on renal fibrosis and the potential mechanisms involved. Methods: In a unilateral ureteral obstruction (UUO)-induced renal fibrosis model, telocytes were injected via the tail vein every other day for 10 days. The degree of renal damage and fibrosis was determined using histological assessment. The expression of collagen I, fibronectin, epithelial-mesenchymal transition markers, and Smad2/3 phosphorylation was examined by western blot analyses. Real-time PCR and enzyme-linked immunosorbent assay were performed in vivo to detect the levels of transforming growth factor (TGF)-β1 and various growth factors. Results: Telocytes attenuated renal fibrosis, as evidenced by reduced interstitial collagen accumulation, decreased expression of fibronectin and collagen I, upregulation of E-cadherin, and downregulation of α-smooth muscle actin. Furthermore, telocytes decreased serum TGF-β1 levels, suppressed Smad2/3 phosphorylation, and increased the expression of hepatocyte growth factor (HGF) in rat kidney tissue following UUO. Blockage of HGF counteracted the protective effect of telocytes on UUO-treated kidneys. Through the detection of HGF mRNA levels in vitro, we found that telocytes had no effect on HGF expression compared with renal fibroblasts. Conclusion: Telocytes attenuated UUO-induced renal fibrosis in rats, likely through enhancing the expression of HGF in an indirect manner.

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“…Even though telocyte presence within a given tissue could be confirmed using several techniques, such as immunofluorescence, immunostaining or proteomics and genetic methods transmission electron microscopy remains a valuable diagnostic tool in identifying this cell population 34,61–63.…”

Section: Discussionmentioning

confidence: 99%

Electron microscopy of human fascia lata: focus on telocytes

Dawidowicz¹,

Szotek

Matysiak

et al. 2015

J Cellular Molecular Medi

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From the histological point of view, fascia lata is a dense connective tissue. Although extracellular matrix is certainly the most predominant fascia’s feature, there are also several cell populations encountered within this structure. The aim of this study was to describe the existence and characteristics of fascia lata cell populations viewed through a transmission electron microscope. Special emphasis was placed on telocytes as a particular interstitial cell type, recently discovered in a wide variety of tissues and organs such as the heart, skeletal muscles, skin, gastrointestinal tract, uterus and urinary system. The conducted study confirmed the existence of a telocyte population in fascia lata samples. Those cells fulfil main morphological criteria of telocytes, namely, the presence of very long, thin cell processes (telopodes) extending from a relatively small cell body. Aside from telocytes, we have found fibroblasts, mast cells and cells with features of myofibroblastic differentiation. This is the first time it has been shown that telocytes exist in human fascia. Currently, the exact role of those cells within the fascia is unknown and definitely deserves further attention. One can speculate that fascia lata telocytes likewise telocytes in other organs may be involved in regeneration, homeostasis and intracellular signalling.

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miR-193 expression differentiates telocytes from other stromal cells

Cited by 108 publications

References 25 publications

Telocytes

Telocytes

Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats

Electron microscopy of human fascia lata: focus on telocytes

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