miR‐193: A new weapon against cancer

Khordadmehr, Monireh; Shahbazi, Roya; Sadreddini, Sanam; Baradaran, Behzad

doi:10.1002/jcp.28368

Cited by 70 publications

(61 citation statements)

References 123 publications

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“…In our study, miR-193a-5p and miR-193b were upregulated in the PAC-resistant cell line, which suggests they can regulate the expression of genes involved in resistance to this drug. As demonstrated by Khordadmehr et al, the miR-193 family (miR-193a-3p, miR-193a-5p, miR-193b3p, and miR-193b-5p) plays an important role in the pathogenesis of ovarian cancer [70]. A similar result was obtained for miR-23 family members, miR-23b and miR-23c.…”

Section: Discussionsupporting

confidence: 65%

The Significance of MicroRNAs Expression in Regulation of Extracellular Matrix and Other Drug Resistant Genes in Drug Resistant Ovarian Cancer Cell Lines

Kaźmierczak

Jopek

Sterzyńska

et al. 2020

IJMS

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Ovarian cancer rates the highest mortality among all gynecological malignancies. The main reason for high mortality is the development of drug resistance. It can be related to increased expression of drug transporters and increased expression of extracellular matrix (ECM) proteins. Our foremost aim was to exhibit alterations in the miRNA expression levels in cisplatin (CIS), paclitaxel (PAC), doxorubicin (DOX), and topotecan (TOP)-resistant variants of the W1 sensitive ovarian cancer cell line-using miRNA microarray. The second goal was to identify miRNAs responsible for the regulation of drug-resistant genes. According to our observation, alterations in the expression of 40 miRNAs were present. We could observe that, in at least one drug-resistant cell line, the expression of 21 miRNAs was upregulated and that of 19 miRNAs was downregulated. We identified target genes for 22 miRNAs. Target analysis showed that miRNA regulates key genes responsible for drug resistance. Among others, we observed regulation of the ATP-binding cassette subfamily B member 1 gene (ABCB1) in the paclitaxel-resistant cell line by miR-363 and regulation of the collagen type III alpha 1 chain gene (COL3A1) in the topotekan-resistant cell line by miR-29a.

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Section: Discussionsupporting

confidence: 65%

The Significance of MicroRNAs Expression in Regulation of Extracellular Matrix and Other Drug Resistant Genes in Drug Resistant Ovarian Cancer Cell Lines

Kaźmierczak

Jopek

Sterzyńska

et al. 2020

IJMS

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“…In our study miR-193a-5p miR-193b are upregulated in PACresistant cell line that suggest they can regulate expression of gene involved in resistance to this drug. As demonstrated by Khordadmehr et al, the miR-193 family (miR-193a-3p, miR-193a-5p, miR-193b3p, and miR-193b-5p) plays an important role in the pathogenesis of ovarian cancer [76]. A similar result was obtained for miR-23 family members -miR-23b and miR-23c.…”

Section: Discussionsupporting

confidence: 64%

The significance of microRNA genes expression in ovarian cancer cell lines resistant to cytotoxic drugs

Kaźmierczak¹,

Jopek²,

Sterzyńska³

et al. 2019

Preprint

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Ovarian cancer is the most fatal type of gynecological cancer. The main reason for high mortality is the development of drug resistance. It can be related to increased expression of drug transporters and increased expression of extracellular matrix (ECM) proteins. miRNA is a short noncoding RNA that regulates expression of about 60% of genes in the human genome and plays a crucial role in developing cancer, including resistance to chemotherapy.Our foremost aim was to exhibit alterations in the miRNA expression levels in the cisplatin (CIS), paclitaxel (PAC), doxorubicin (DOX), and topotecan (TOP) -resistant variants of W1 sensitive ovarian cancer cell line using miRNA microarray. The second goal was to identify miRNAs responsible for the regulation of drug-resistant genes.According to our observation, alterations in the expression of 40 miRNA genes. The level of expression of 21 genes was upregulated in at least one drug-resistant cell line. The expression of 19 genes was downregulated in at least one drug-resistant cell line. We identified target genes for 22 miRNAs. Target analysis showed that miRNA regulates key genes responsible for drug resistance. Among others, we observed regulation of the ABCB1 (MDR1) gene in the W1PR1 cell line by miR-363 and regulation of the COL3A1 gene in the W1TR cell line by miR-29a.Hence, on the basis of our findings, results indicate that genes responsible for drug resistance development in ovarian cancer can be regulated by miRNA.

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“…[19][20][21] Several miRNAs have been identified as being associated with different cancers, including OS, 22,23 and there is evidence that miR-193b acts as a tumor suppressor in various types of cancer. [24][25][26][27][28][29][30] In the present study, we demonstrated that miR-193b expression is lower in OS specimens than in adjacent normal tissue. To better characterize the role of miR-193b, we then conducted functional studies, and showed that miR-193b overexpression induces apoptosis in OS cells, while miR-193b knockdown yields the opposite effect in vitro.…”

Section: Discussionmentioning

confidence: 73%

<p>miR-193b Increases the Chemosensitivity of Osteosarcoma Cells by Promoting FEN1-Mediated Autophagy</p>

Dong¹,

Ye²,

Ma³

et al. 2019

OTT

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Background: Osteosarcoma (OS) is one of the most common malignant bone tumors and specific microRNAs (miRNAs) are closely associated with malignant OS progression. In this study, we examined the role of and the involvement of autophagy and apoptosis in the chemosensitivity of OS cells. Methods: We employed qRT-PCR, Western blot, and immunohistochemistry to examine the expression levels of miR-193b, flap endonuclease 1 (FEN1), and autophagy-related proteins. Apoptosis was determined by flow cytometry using an Annexin V-FITC/PI apoptosis detection kit. Luciferase reporter assays confirmed the relationship between miR-193b and FEN1. Results: miR-193b was downregulated in OS compared to adjacent normal tissues (p < 0.05). miR-193b overexpression in the OS cell lines induced autophagy and apoptosis, as shown by Western blotting and flow cytometry. Knockdown of FEN1, a structure-specific nuclease overexpressed in OS tissues (p < 0.001), induced apoptosis through activation of autophagy. Luciferase reporter assays confirmed that FEN1 is a direct target of miR-193b, FEN1 knockdown reinforced miR-193b induced apoptosis. Moreover, miR-193b expression enhanced epirubicin-induced autophagy and apoptosis. Conclusion: Collectively, the results showed that miR-193b/FEN1 may serve as a novel therapeutic target for OS aimed mainly at the induction of autophagy and apoptosis. The miR-193b/FEN1 axis increased the chemosensitivity of OS cells, while activation of autophagy enhanced the anticancer effects of epirubicin.

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miR‐193: A new weapon against cancer

Cited by 70 publications

References 123 publications

The Significance of MicroRNAs Expression in Regulation of Extracellular Matrix and Other Drug Resistant Genes in Drug Resistant Ovarian Cancer Cell Lines

The Significance of MicroRNAs Expression in Regulation of Extracellular Matrix and Other Drug Resistant Genes in Drug Resistant Ovarian Cancer Cell Lines

The significance of microRNA genes expression in ovarian cancer cell lines resistant to cytotoxic drugs

<p>miR-193b Increases the Chemosensitivity of Osteosarcoma Cells by Promoting FEN1-Mediated Autophagy</p>

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