miR-185-3p regulates the invasion and metastasis of nasopharyngeal carcinoma by targeting WNT2B in vitro

Abstract

MicroRNAs (miRs) have been recognised as important regulators of malignant behaviour in different types of human cancer, including nasopharyngeal carcinoma (NPC). A previous study by our group revealed that miR-185-3p regulates the radioresistance of NPC cells. The present study aimed to investigate the effect of miR-185-3p on NPC invasion and metastasis. Human NPC CNE-2 and 5-8F cell lines were transfected with a miR-185-3p mimic and miR-185-3p inhibitor, respectively, and their effects on the invasion and metastasis of these cells was assessed using a wound healing assay and Matrigel invasion assay. The target gene of miR-185-3p, Wnt family member 2B (WNT2B) was silenced in 5-8F cells using siRNA in order to investigate its function in NPC. Data from the present study demonstrated that the expression of miR-185-3p was the highest in 5-8F and lowest in CNE-2 cells out of a range of NPC cell lines. Following the transfection of miR-185-3p mimic into CNE-2 cells, the wound healing and Matrigel invasion assays indicated that the migration and invasion ability of CNE-2 cells was significantly reduced compared with the negative control group. In addition, the inhibition of miR-185-3p in 5-8F cells significantly increased the capacity for migration and invasion. Furthermore, silencing WNT2B expression resulted in a significant reduction in the invasion and metastasis in 5-8F cells. The inhibition of miR-185-3p, which promotes invasion and metastasis, could be reversed through the silencing of WNT2B in 5-8F cells. The results of the present study indicate that miR-185-3p mediates the invasion and metastasis of NPC by targeting WNT2B in vitro.

miR‐206 enhances nasopharyngeal carcinoma radiosensitivity by targeting IGF1

“…**P < 0.01; ***P < 0.001. miR-504 is up-regulated in NPC radio-resistant cells and could directly down-regulated the expression of NRF1 and regulated mitochondrial-mediated oxidative responses, allowing NPC cells to gain radio-resistant characteristics [14]. miR-185-3p and miR-205 are also found to correlate with NPC radioresistance [15,16]. In the present study, we found miR-206 was downregulated in radioresistant NPC cells.…”

Effects of Epigallocatechin Gallate (EGCG) on Urinary Bladder Urothelial Carcinoma―Next-Generation Sequencing and Bioinformatics Approaches

“…Kallifatidis et al recently reported increased ARRB1 expression in bladder cancer cells, and ARRB1 gene knockout reversed the aggressive phenotype of bladder cancer cell lines [37]. MiR-185-3p is a potential oncosuppressor miRNA evident in nasopharyngeal carcinoma (NPC), and the inhibition of miR-185-3p promoted invasion and metastasis of NPC cells [38]. In the current study, altered miR-185-3p-ARRB1 interaction was identified in EGCG-treated bladder TCC cells, with 3.92-fold up-regulated miR-185-3p and 4.01-fold suppressed ARRB1.…”

Wnt3a promotes radioresistance via autophagy in squamous cell carcinoma of the head and neck

“…After irradiation, the cells were cultured for another 12‐14 days and the number of surviving colonies (defined as a colony with > 50 cells) was counted. The survival fraction was calculated as we previously described . Each experiment was performed in duplicate or triplicate.…”

“…The survival fraction was calculated as we previously described. 14,17 Each experiment was performed in duplicate or triplicate.…”