MiR-183-5p Promotes the Cell Proliferation of Colorectal Cancer by Targeting QKI-5

Abstract

Background Colorectal cancer is the third most common malignant tumor in the world and the fourth most common cancer-related death cause. Previous studies have confirmed that miR-183-5P is considered to be a cancer-associated miRNA in several tumor types. Methods We analyzed the expression of miR-183-5P in cancer tissues and adjacent tissues in TCGA database. The expression of miR-183-5P and QKI-5 mRNA was measured by RT-qPCR and western blot. CCK-8 were used to indicate the proliferation capacity. BALB/c nude mice were used to simulate in vivo experiments. Results In this study, we identified miR-183-5p as a tumor promoter in colorectal cancer. The expression of miR-183-5p was found upregulated in human colorectal cancer tissues while QKI-5 was down-regulated. CCK-8 assay demonstrated that miR-183-5p promoted colorectal cancer cell proliferation. We also found miR183-5P can target QKI-5 and inhibit its expression in CRC cell lines. Restoration of QKI-5 reversed the effects of miR-183-5p in colorectal cancer cells. Conclusion Taken together, our results suggest that miR-183-5p might function as a tumor-promoting factor in colorectal cancer and might contribute to its proliferation.