miR‐182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro

Abstract

Proper HOXA10 expression was essential for endometrial receptivity what was crucial for successful embryo implantation in mammalian. This study confirmed that miR-182 regulated the expression levels of HOXA10 by binding to its 3' UTR, selectively downregulated HOXA10 in goat endometrial epithelium cells (gEECs) but not stromal cell (gESCs) in vitro. However, HOXA10 and miR-182 both up-expressed in the goat endometrium at gestational day 15 (D15) compared with gestational day 5 (D5), suggesting that there were some other factors regulated the expression of HOXA10 during the development of goat endometrium in vivo. What's more, HOXA10 gene silencing (HOXA10-siRNA) resulted in gEECs apoptosis in vitro, and it regulated the protein levels of oestrogen receptor a (ERa), progesterone receptor B (PRb), insulin-like growth factor 1 receptor (IGF1R), BCL-2, pleiotrophin (PTN), AKT and p-JNK in gEECs. Furthermore, HOXA10 might regulate the protein levels of endometrial receptivity biomarker genes, including vascular endothelial growth factor (VEGF), osteopontin (OPN), cyclooxygenase-2 (COX-2) and prolactin receptor (PRLR) in gEECs. In conclusion, miR-182 targeted HOXA10 selectively in EECs in vitro, and HOXA10 played an important role in maintaining the function of EECs in dairy goats.

Temporal transcriptomic changes in microRNAs involved in the host immune response and metabolism during Neospora caninum infection

“…ovary [ 45 ], liver [ 46 ], pituitary [ 47 ], lung [ 48 ]) and types of cells (e.g. skeletal muscle satellite cells [ 49 ], skin fibroblast cells [ 50 ], Leydig cells [ 51 ], blood leukocytes and milk somatic cells [ 52 ], intramuscular preadipocytes [ 53 ], endometrial epithelium cells [ 54 ]) in physiological and pathological processes in goats, including in response to infectious diseases. For example, the expression of 316 DEmiRNAs was found in peripheral blood mononuclear cells of goats infected with peste des petits ruminants virus, and most of the predicted targets of these DEmiRNAs were found to be involved in immune escape [ 55 ].…”

Homeobox A10 promotes the proliferation and invasion of bladder cancer cells via regulation of matrix metalloproteinase‑3

“…Therefore, future studies should focus on the mechanisms underlying the regulation of MMP-3 by HOXA10 and the effect of HOXA10 overexpression on BC cell behavior. Furthermore, HOXA10 has been reported to be regulated by various miRNAs in different tumors and to be involved in different signaling pathways (19,30,31), which may be an important study direction in the future.…”

Testin was regulated by circRNA3175‐miR182 and inhibited endometrial epithelial cell apoptosis in pre‐receptive endometrium of dairy goats

“…For example, Let‐7g was reported to target Caspase‐3, and inhibit the apoptosis induced by oxidized low‐density lipoprotein ( OX‐LDL ) in endothelial cells (Zhang, Chen, Zhang, & Tong, ). miR‐182 could induce EEC apoptosis by directly targeting the 3′‐untranslated region (3′‐UTR) of PTN (Zhang, Liu, Liu, Ma, et al, ), and directly regulate HOXA10 via its 3′‐UTR (Zhang, Liu, Liu, Song, et al, ).…”