miR-182-5p affects human bladder cancer cell proliferation, migration and invasion through regulating Cofilin 1

Wang, Fei; Wu, Dinglan; Zhou, Xu; Chen, Jianxiang; Zhang, Jiye; Li, Xiaojuan; Chen, Shiliang; He, Feifei; Xu, Jianbing; Su, Liangju; Luo, Defan; Zhang, Shufang; Wang, Weifu

doi:10.1186/s12935-019-0758-5

Cited by 38 publications

(32 citation statements)

References 42 publications

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“…Besides, accumulated evidence indicated that miRNAs also have a pivotal role in the development of cancer [25]. It has been revealed that miR-182-5p can affect human bladder cancer cell proliferation, migration, and invasion [26]. Furthermore, miRNAs have involved in lncRNAs.…”

Section: Discussionmentioning

confidence: 99%

Up-regulated Linc00472 suppresses development of lung cancer cell via inhibition of MiR-196b-5p

Mao¹,

Zhou²,

Li³

et al. 2019

Bioscience, Biotechnology, and Biochemistry

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The role of linc00472 in lung cancer (LC) has been rarely reported. We aimed to study the role of linc00472 in LC progression. Expressions of linc00472 and miR-196b-5p in LC cell lines were measured by qRT-PCR. The targeting relationship between linc00472 and miR-196b-5p was determined by Starbase and dual-luciferase reporter. The viability, migration, invasion, and apoptosis of LC cells were determined using CCK-8 assay, scratch test, transwell assay, and flow cytometry, respectively. The levels of epithelial-to-mesenchymal transition (EMT)-related proteins and apoptosis-related proteins in LC cells were determined by western blot. Downregulated linc00472 was observed in five LC cell lines. Linc00472 overexpression suppressed viability, migration, invasion and EMT process, but elevated apoptotic rate in LC cells. MiR-196b-5p mimic promoted viability, migration, invasion, and EMT process, but decreased apoptotic rate, which was reversed by up-regulated linc00472. Linc00472 functioned as a cancer suppressor via negatively regulating miR-196b-5p of LC cells.

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Section: Discussionmentioning

confidence: 99%

Up-regulated Linc00472 suppresses development of lung cancer cell via inhibition of MiR-196b-5p

Mao¹,

Zhou²,

Li³

et al. 2019

Bioscience, Biotechnology, and Biochemistry

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“…These results indicated that glioma-secreted exosomes can be effectively absorbed by endothelial cells. miR-182-5p has been reported to be involved in tumor proliferation (47)(48)(49)(50)(51)(52)(53), invasion (47,49,51), and metastasis (48,(54)(55)(56)(57). However, the role of miR-182-5p in vascular endothelial cells remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Hypoxic Cancer-Secreted Exosomal miR-182-5p Promotes Glioblastoma Angiogenesis by Targeting Kruppel-like Factor 2 and 4

Yuan

et al. 2020

Molecular Cancer Research

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Glioblastoma (GBM) is the most lethal primary brain tumor and has a complex molecular profile. Hypoxia plays a critical role during tumor progression and in the tumor microenvironment (TME). Exosomes released by tumor cells contain informative nucleic acids, proteins, and lipids involved in the interaction between cancer and stromal cells, thus leading to TME remodeling. Accumulating evidence indicates that exosomes play a pivotal role in cell-to-cell communication. However, the mechanism by which hypoxia affects tumor angiogenesis via exosomes derived from tumor cells remains largely unknown. In our study, we found that, compared with the parental cells under normoxic conditions, the GBM cells produced more exosomes, and miR-182-5p was significantly upregulated in the exosomes from GBM cells under hypoxic conditions. Exosomal miR-182-5p directly suppressed its targets Kruppel-like factor 2 and 4, leading to the accumulation of VEGFR, thus promoting tumor angiogenesis. Furthermore, exosome-mediated miR-182-5p also inhibited tight junction-related proteins (such as ZO-1, occludin, and claudin-5), thus enhancing vascular permeability and tumor transendothelial migration. Knockdown of miR-182-5p reduced angiogenesis and tumor proliferation. Interestingly, we found elevated levels circulating miR-182-5p in patient blood serum and cerebrospinal fluid samples, and its expression level was inversely related to the prognosis. Implications: Overall, our data clarify the diagnostic and prognostic value of tumor-derived exosome-mediated miR-182-5p and reveal the distinctive cross-talk between tumor cells and human umbilical vein endothelial cells mediated by tumorderived exosomes that modulate tumor vasculature.

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“…Similarly, Li et al reported overexpression of miR-182-5p in gastric cancer and its promoting effect on cell migration and invasion [30]. On the contrary, Wang et al demonstrated that miR-182-5p had a lower expression in bladder cancer tissues than in the matched normal tissues and its up-regulation reduced cell proliferation and invasion [31]. Thus, miR-182-5p may play different roles during cancer development with different cellular environment.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA MTO1 inhibits the proliferation and invasion of ovarian cancer cells through the miR-182-5p/KLF15 axis

Wang

Cao

Tian

et al. 2020

Preprint

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Background: Circular RNAs (circRNAs) are a novel class of endogenous noncoding RNAs and have been shown to play important roles in a variety of physiological processes. Recently, dysregulation of circRNAs has been identified in many types of cancers. In this study, we analyzed the expression profile and biological functions of circMTO1 in ovarian cancer. Materials and methods: Cell proliferation and invasion were assessed by CCK-8 and transwell assay, respectively. The RT-qPCR analysis was performed to measure mRNA levels of circMTO1 and miR-182-5p. The western blot was used to detect KLF15 protein levels. Luciferase reporter assays were performed to determine the interaction between LINC00958 and miR-204-3p and the interaction between miR-204-3p and KIF2A. Results: We demonstrated that circMTO1 was down-regulated in ovarian cancer tissues and cell lines. Up-regulation of circMTO1 inhibited proliferation and invasion of ovarian cancer cells while down-regulation of circMTO1 promoted these processes. Mechanistically, we showed that circMTO1 sponged miR-182-5p to support KLF15 expression, eventually leading to inhibition of ovarian cancer progression. Conclusions: Our study suggested circMTO1 as a novel biomarker and therapeutic target for ovarian cancer treatment.

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miR-182-5p affects human bladder cancer cell proliferation, migration and invasion through regulating Cofilin 1

Cited by 38 publications

References 42 publications

Up-regulated Linc00472 suppresses development of lung cancer cell via inhibition of MiR-196b-5p

Up-regulated Linc00472 suppresses development of lung cancer cell via inhibition of MiR-196b-5p

Hypoxic Cancer-Secreted Exosomal miR-182-5p Promotes Glioblastoma Angiogenesis by Targeting Kruppel-like Factor 2 and 4

Circular RNA MTO1 inhibits the proliferation and invasion of ovarian cancer cells through the miR-182-5p/KLF15 axis

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