MiR-181c affects estrogen-dependent endometrial carcinoma cell growth by targeting PTEN

Abstract

MicroRNAs (miRNAs), which is a type of non-coding and single-stranded small molecule RNA, bind either completely or incompletely to 3'-UTR of the target gene mRNA to inhibit mRNA translation or degradation. In our study, we aimed to explore the roles and mechanisms of miR-181c in the apoptosis of RL95-2 human endometrial carcinoma cells. Cell activity and apoptosis were detected by cell counting Kit-8 (CCK-8) assay and flow cytometry (FCM), respectively. Related mRNAs and proteins expression was determined by quantitative real-time reverse transcription PCR (qRT-PCR) and western blot assays, respectively. The binding capacity of PTEN-3'-UTR and miR-181c was assessed by luciferase reporter assay. The obtained results suggested that E2 evidently increased the cell activity of RL95-2 cells. In addition, miR-181c inhibitor suppressed the cell viability and enhanced the apoptosis capacity of E2-induced RL95-2 cells and distinctly reduced the miR-181c expression. We also found that miR-181c could bind to PTEN-3'-UTR and miR-181c inhibitor up-regulated the expression level of PTEN in E2-induced RL95-2 cells. Besides, overexpression of PTEN markedly promoted the apoptosis of E2-induced RL95-2 cells through regulating the Bax and Bcl-2 expression, and modulated the expression of AKT pathway, p53 and Cyclin D. In conclusion, our findings revealed that miR-181c affected the estrogen-dependent endometrial carcinoma cell growth by targeting PTEN. The potential effects of miR-181c on the apoptosis of E2-induced RL95-2 cells suggest that miR-181c could be an effective target for endometrial carcinoma therapies.

The Role of miR-181c in Mechanisms of Diabetes-Impaired Angiogenesis: An Emerging Therapeutic Target for Diabetic Vascular Complications

“…PTEN negatively regulates the PI3K/Akt pathway to inhibit proliferation. miR-181c has also been shown to target PTEN in endometrial cancer (Zhuang et al, 2019), potentiating cell survival through reduced apoptosis. In response to hypoxia, suppression of miR-181c promoted cardiomyocyte survival by increasing phosphorylation of Akt and also upregulating the cell survival mediator, Bcl-2 (Li X. et al, 2020).…”

Establishment of the Prognosis Predicting Signature for Endometrial Cancer Patient

“…Through which, these miRNAs contribute to the regulation of cancer cell proliferation or progression [13,14]. MiRNAs, such as miR-181c and miR-522, have been found that play a critical role during the tumorigenesis or progression of EC [15,16]. Studies also pointed out that miRNAs’ features indicate a possible role as prognosis-related markers.…”

Changes in Dna Methylation Profile in Tamoxifen-Resistant McF-7 Sublines

“…Анализируя вопрос об эпигенетических факторах, участвующих в формировании гормональной резистентности, нельзя не отметить микроРНК как класс биологически активных соединений, чью роль в развитии и поддержании резистентности трудно переоценить. Среди них: mir-27, mir-181, mir-221/222, mir-21, mir-101 и ряд других, обладающих широким спектром действия, в первую очередь на PI3K/Akt/mTOR сигнальный путь, и непосредственно участвующих в развитии и поддержании гормональной резистентности [30][31][32][33]. Активно разрабатываются и диагностические тесты на определение гормональной зависимости опухолей, включающие сигнатуру из нескольких видов микроРНК [34,35].…”