MiR-181b suppresses angiogenesis by directly targeting cellular communication network factor 1

Li, Yue; Fan, Siyuan; Xia, Weichang; Qiao, Baoru; Huang, Kai; Zhou, Jingqun; Liang, Minglu

doi:10.1038/s41374-021-00596-4

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…The protein encoded by CCND1 is cyclin D1, which belongs to the highly conserved cyclin family [ 29 ]. The communication network factor 1(CCN1), also known as CYR61 (cysteine-rich protein 61), is the first identified member of the CCN family and the first member described to be an angiogenic factor [ 30 ]. It indicates that Cyclin D1 and CCN1 may play a key role in thrombin-induced smooth muscle cell proliferation and angiogenesis.…”

Section: Resultsmentioning

confidence: 99%

Integrative analysis of HASMCs gene expression profile revealed the role of thrombin in the pathogenesis of atherosclerosis

Zhang

Sun

Wang

et al. 2023

BMC Cardiovasc Disord

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We explored the effect of thrombin on human aortic smooth muscle cells (HASMCs) and further analyzed its role in the pathogenesis of atherosclerosis (AS). Thrombin-induced differentially expressed genes (DEGs) in HASMCs were identified by analyzing expression profiles from the GEO. Subsequently, enrichment analysis, GSEA, PPI network, and gene-microRNAs networks were interrogated to identify hub genes and associated pathways. Enrichment analysis results indicated that thrombin causes HASMCs to secrete various pro-inflammatory cytokines and chemokines, exacerbating local inflammatory response in AS. Moreover, we identified 9 HUB genes in the PPI network, which are closely related to the inflammatory response and the promotion of the cell cycle. Additionally, we found that thrombin inhibits lipid metabolism and autophagy of HASMCs, potentially contributing to smooth muscle-derived foam cell formation. Our study deepens a mechanistic understanding of the effect of thrombin on HASMCs and provides new insight into treating AS.

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Section: Resultsmentioning

confidence: 99%

Integrative analysis of HASMCs gene expression profile revealed the role of thrombin in the pathogenesis of atherosclerosis

Zhang

Sun

Wang

et al. 2023

BMC Cardiovasc Disord

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“…In addition, a zebrafish xenograft model can also be used to investigate angiogenesis in vivo. In a mouse model, Matrigel plug assays are generally used for the assessment of angiogenesis in vivo, which requires seven days [ 67 ]. For zebrafish xenografts, we used EGFP-labeled vascular endothelial cell transgenic lines for transplantation, and it is easy to observe the changes in blood vessels in vivo by fluorescence microscopy [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

The RNA demethylase ALKBH5 promotes the progression and angiogenesis of lung cancer by regulating the stability of the LncRNA PVT1

Shen

Zuo

et al. 2022

Cancer Cell Int

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Background N6-methyladenosine (m6A) is the most common posttranscriptional modification of RNA and plays critical roles in human cancer progression. However, the biological function of m6A methylation requires further studied in cancer, especially in tumor angiogenesis. Methods A public database was used to analyze the expression and overall survival of ALKBH5 and PVT1 in lung cancer patients. CCK-8 and colony formation assays were performed to detect cell proliferation, a transwell assay was used to assess cell migration, and a tube formation assay was performed to assess angiogenic potential in vitro. A zebrafish lung cancer xenograft model was used to verify the function of ALKBH5 and PVT1 in vivo. Western blot assays were used to measure the relative protein expression in lung cancer cells. SRAMP predictor analysis and RNA stability experiments were used to examine the potential m6A modification. Results Bioinformatics analysis showed that the expression levels of m6A-related genes were changed significantly in lung cancer tissues compared with normal lung tissues. We then identified that ALKBH5 was upregulated in lung cancer tissues and associated with poor prognosis of lung cancer patients by analyzing a public database. Knockdown of ALKBH5 inhibited the proliferation and migration of cultured lung cancer cell lines. Zebrafish lung cancer xenografts showed that ALKBH5 silencing also suppressed the growth and metastasis of lung cancer cells. Moreover, knockdown of ALKBH5 inhibited the angiogenesis of lung cancer in vitro and in vivo. Mechanistic studies showed that knockdown of ALKBH5 decreased the expression and stability of PVT1 in lung cancer cells. We next observed that PVT1 promoted the progression of lung cancer cells in vitro and in vivo and regulated the expression of VEGFA and angiogenesis in lung cancer. Finally, rescue experiments revealed that ALKBH5 regulated the proliferation, migration and angiogenesis of lung cancer cells, partially through PVT1. Conclusion Our results demonstrate that ALKBH5 promotes the progression and angiogenesis of lung cancer by regulating the expression and stability of PVT1, which provides a potential prognostic and therapeutic target for lung cancer patients.

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“…miR-181b plays an essential role in the regulation of angiogenesis in different types of tumors [ 54 , 55 ]. However, whether miR-181b modulation could exert an antiangiogenic and pro-apoptotic action in ACC remains to be clarified.…”

Section: Tumorigenesis Pathways In Which Five Of the Main Acc Circula...mentioning

confidence: 99%

MicroRNAs and Long Non-Coding RNAs in Adrenocortical Carcinoma

Detomas

Pivonello

Pellegrini

et al. 2022

Cells

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Non-coding RNAs (ncRNAs) are a type of genetic material that do not encode proteins but regulate the gene expression at an epigenetic level, such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). The role played by ncRNAs in many physiological and pathological processes has gained attention during the last few decades, as they might be useful in the diagnosis, treatment and management of several human disorders, including endocrine and oncological diseases. Adrenocortical carcinoma (ACC) is a rare and aggressive endocrine cancer, still characterized by high mortality and morbidity due to both endocrine and oncological complications. Despite the rarity of this disease, recently, the role of ncRNA has been quite extensively evaluated in ACC. In order to better explore the role of the ncRNA in human ACC, this review summarizes the current knowledge on ncRNA dysregulation in ACC and its potential role in the diagnosis, treatment, and management of this tumor.

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MiR-181b suppresses angiogenesis by directly targeting cellular communication network factor 1

Cited by 6 publications

References 19 publications

Integrative analysis of HASMCs gene expression profile revealed the role of thrombin in the pathogenesis of atherosclerosis

Integrative analysis of HASMCs gene expression profile revealed the role of thrombin in the pathogenesis of atherosclerosis

The RNA demethylase ALKBH5 promotes the progression and angiogenesis of lung cancer by regulating the stability of the LncRNA PVT1

MicroRNAs and Long Non-Coding RNAs in Adrenocortical Carcinoma

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