MiR-17 and miR-19 cooperatively promote skeletal muscle cell differentiation

Kong, Delin; He, Mei; Yang, Lin; Zhou, Rongtao; Yan, Yunjun; Yang, Liang; Teng, Chun‐Bo

doi:10.1007/s00018-019-03165-7

Cited by 46 publications

(37 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Muscle-specific miRNAs included miR-1, miR-133a/b, and miR-206, which were involved in myoblast proliferation and differentiation by regulating the expression of target genes, such as histone deacetylase 4, serum response factor, the myogenic differentiation family, and other myogenic transcriptional factors [9][10][11]. Other non-specific muscle miRNAs have also been involved in the regulation of muscle growth and development, such as miR-17, miR-19, miR-15b, miR-322, miR-208b, miR-26a, miR-30, miR-128a, miR-203a, miR-214, and miR-3906 [12][13][14][15]. For example, miR-26a promoted myoblast differentiation by regulating the expression of target genes Smad1 and Smad4 [16].…”

Section: Introductionmentioning

confidence: 99%

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Jia

Liu

et al. 2020

Genes

View full text Add to dashboard Cite

Studies of the gene and miRNA expression profiles associated with the postnatal late growth, development, and aging of skeletal muscle are lacking in sika deer. To understand the molecular mechanisms of the growth and development of sika deer skeletal muscle, we used de novo RNA sequencing (RNA-seq) and microRNA sequencing (miRNA-seq) analyses to determine the differentially expressed (DE) unigenes and miRNAs from skeletal muscle tissues at 1, 3, 5, and 10 years in sika deer. A total of 51,716 unigenes, 171 known miRNAs, and 60 novel miRNAs were identified based on four mRNA and small RNA libraries. A total of 2,044 unigenes and 11 miRNAs were differentially expressed between adolescence and juvenile sika deer, 1,946 unigenes and 4 miRNAs were differentially expressed between adult and adolescent sika deer, and 2,209 unigenes and 1 miRNAs were differentially expressed between aged and adult sika deer. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DE unigenes and miRNA were mainly related to energy and substance metabolism, processes that are closely associate with the growth, development, and aging of skeletal muscle. We also constructed mRNA–mRNA and miRNA–mRNA interaction networks related to the growth, development, and aging of skeletal muscle. The results show that mRNA (Myh1, Myh2, Myh7, ACTN3, etc.) and miRNAs (miR-133a, miR-133c, miR-192, miR-151-3p, etc.) may play important roles in muscle growth and development, and mRNA (WWP1, DEK, UCP3, FUS, etc.) and miRNAs (miR-17-5p, miR-378b, miR-199a-5p, miR-7, etc.) may have key roles in muscle aging. In this study, we determined the dynamic miRNA and unigenes transcriptome in muscle tissue for the first time in sika deer. The age-dependent miRNAs and unigenes identified will offer insights into the molecular mechanism underlying muscle development, growth, and maintenance and will also provide valuable information for sika deer genetic breeding.

show abstract

Section: Introductionmentioning

confidence: 99%

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Jia

Liu

et al. 2020

Genes

View full text Add to dashboard Cite

show abstract

“…For instance, miR-19b was shown to negatively regulate PLZF expression in goat mGSCs and induce goat mGSC proliferation [22]. Kong also pointed out that miR-19 promoted skeletal muscle cell differentiation cooperatively with miR-17 [23]. In addition, it was reported that miR-19b could suppress apoptosis and promote the proliferation of T-cell lines.…”

Section: Discussionmentioning

confidence: 99%

Regulation of the miR-19b-mediated SOCS6-JAK2/STAT3 pathway by lncRNA MEG3 is involved in high glucose-induced apoptosis in hRMECs

Xiao

Lan

et al. 2020

Bioscience Reports

View full text Add to dashboard Cite

Objective: Diabetic retinopathy (DR) is one of the most severe and common complications of diabetes mellitus. The present study aimed to investigate the molecular mechanism of MEG3, miR-19b and SOCS6 in human retinal microvascular endothelial cells (hRMECs) under high glucose conditions. Methods: HRMECs were cultured in 5 or 30 mM D-glucose medium. qRT-PCR and Western blotting were used to determine the mRNA expression and protein levels. MTT assay and flow cytometry analysis were performed to detect the viability and apoptosis of hRMECs, respectively. TNF-α, IL-6 and IL-1β levels in cell supernatants were detected by ELISA. The activity of caspase-3/7 was also determined. A luciferase reporter assay was performed to confirm the targeting relationship between miR-19b and SOCS6, as well as MEG3 and miR-19b. Results: Our study demonstrated that miR-19b was increased and SOCS6 was decreased in HG-induced hRMECs. Knockdown of SOCS6 inhibited cell viability and reversed the promotion of cell viability induced by knockdown of miR-19b. Additionally, miR-19b directly targeted and negatively regulated SOCS6. Moreover, miR-19b promoted the cell apoptosis rate and caspase-3/7 activity and increased inflammatory factors through the SOCS6-mediated JAK2/STAT3 signalling pathway. In addition, MEG3 attenuated HG-induced apoptosis of hRMECs by targeting the miR-19b/SOCS6 axis. Conclusion: These findings indicate that MEG3 inhibited HG-induced apoptosis and inflammation by regulating the miR-19b/SOCS6 axis through the JAK2/STAT3 signalling pathway in hRMECs. Thus, these findings might provide a new target for the treatment of DR.

show abstract

“…In summary, there have been significant advances in our understanding of the crucial roles of miRNAs and lncRNAs in regulating skeletal muscle development (Cesana et al, 2011;Legnini et al, 2014;Zhu et al, 2017;Kong et al, 2019;Cheng et al, 2020;Li et al, 2020). A limited number of studies have specifically identified roles of miRNAs and lncRNAs in muscular atrophy in cachexia and other diseases, and these studies suggest that miRNAs and lncRNAs are central to abnormal regulation of protein synthesis and degradation.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Skeletal Muscle Atrophy in Cachexia by MicroRNAs and Long Non-coding RNAs

Chen

Liu

Jiang

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Skeletal muscle atrophy is a common complication of cachexia, characterized by progressive bodyweight loss and decreased muscle strength, and it significantly increases the risks of morbidity and mortality in the population with atrophy. Numerous complications associated with decreased muscle function can activate catabolism, reduce anabolism, and impair muscle regeneration, leading to muscle wasting. microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), types of non-coding RNAs, are important for regulation of skeletal muscle development. Few studies have specifically identified the roles of miRNAs and lncRNAs in cellular or animal models of muscular atrophy during cachexia, and the pathogenesis of skeletal muscle wasting in cachexia is not entirely understood. To develop potential approaches to improve skeletal muscle mass, strength, and function, a more comprehensive understanding of the known key pathophysiological processes leading to muscular atrophy is needed. In this review, we summarize the known miRNAs, lncRNAs, and corresponding signaling pathways involved in regulating skeletal muscle atrophy in cachexia and other diseases. A comprehensive understanding of the functions and mechanisms of miRNAs and lncRNAs during skeletal muscle wasting in cachexia and other diseases will, therefore, promote therapeutic treatments for muscle atrophy.

show abstract

MiR-17 and miR-19 cooperatively promote skeletal muscle cell differentiation

Cited by 46 publications

References 63 publications

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Regulation of the miR-19b-mediated SOCS6-JAK2/STAT3 pathway by lncRNA MEG3 is involved in high glucose-induced apoptosis in hRMECs

Regulation of Skeletal Muscle Atrophy in Cachexia by MicroRNAs and Long Non-coding RNAs

Contact Info

Product

Resources

About