MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1

Dong, Ping; Mai, Yin; Zhang, Zhenyu; Mi, Lin; Wu, Guofang; Chu, Guiyan; Yang, Gongshe; Sun, Shiduo

doi:10.1093/abbs/gmu043

Cited by 39 publications

(45 citation statements)

References 32 publications

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“…For example, the early muscle tissue of Duchenne muscular dystrophy is manifested as muscle ber regeneration and mild lipid droplets, and the late muscle bers are gradually replaced by fat and connective tissue, which seriously affects muscle function. Studies have pointed out that miR-15/16 promotes adipogenesis by targeting FOXO1 and EPT1 genes (28,29). In our results, the overexpression of circINSR in preadipocytes inhibited the expression of key genes for adipogenic differentiation and reduced lipid droplet formation.…”

Section: Discussionsupporting

confidence: 61%

CircINSR regulates fetal bovine muscle and fat development

Shen

Tang

et al. 2020

Preprint

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Background: The level of muscle development directly affects the production efficiency of livestock, and the content of intramuscular fat (IMF) is an important factor affecting meat quality. Nevertheless, the molecular mechanism of embryonic circular RNA in muscle and IMF development remains largely unknown. Methods: In this study, we isolated myoblasts and intramuscular preadipocytes from fetal bovine skeletal muscle. Oil Red O and BODIPY staining were used to identify lipid droplets of preadipocytes, and anti-MyHC immunofluorescence was used to identify myotubes differentiated from myoblasts. Bioinformatics, dual fluorescence reporter system, and RNA immunoprecipitation were used to determine the interactions between circINSR and miR-15/16 family. Molecular and biochemical assays were used to confirm the role of circINSR in myoblasts and intramuscular preadipocytes. Results: We found that the isolated myoblasts and preadipocytes can differentiate normally. Besides, circINSR severed as a sponge of miR-15/16 family, which targeting CyclinD1 and Bcl-2. CircINSR overexpression significantly promoted myoblasts and preadipocytes proliferation, and inhibited cell apoptosis. In addition, circINSR inhibited preadipocytes adipogenesis by alleviating the inhibition of miR-15/16 on target genes FOXO1 and EPT1. Conclusions: Taken together, our study demonstrated circINSR as a regulator of embryonic muscle and IMF development.

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Section: Discussionsupporting

confidence: 61%

CircINSR regulates fetal bovine muscle and fat development

Shen

Tang

et al. 2020

Preprint

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“…Targetscan.org suggested that miR15a could directly bind to the 3′UTR of Foxo1. Dong et al (2014) found that over-expression of miR15a/b interacted with the target region of Foxo1 mRNA in porcine pre-adi-pocytes (Dong et al, 2014). Using a luciferase assay, we confirmed that miR15a directly binds Foxo1 in REC.…”

Section: Discussionmentioning

confidence: 99%

miR15a regulates NLRP3 inflammasome proteins in the retinal vasculature

Curtiss

Liu

Steinle

2018

Experimental Eye Research

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We have previously published that miR15a can reduce inflammatory cytokines, which could be key to diabetic retinal pathology. In this work, we wanted to investigate whether miR15a altered NLR pyrin domain 3 (NLRP3) proteins. Whole retinal lysates from both miR15a overexpressing mice and endothelial cell specific miR15a/16 knockout mice were used to investigate protein levels of forkhead box protein O1 (Foxo1), NLRP3, cleaved caspase 1 and interleukin-1 beta (IL-1β). Primary human retinal endothelial cells (REC) were cultured in normal and high glucose followed by transfection with a miR15a mimic for protein analyses. MiR15a expression was verified by quantitative PCR, and a luciferase binding assay was used to examine whether miR15a directly bound Foxo1. In mouse retinal lysates, loss of miR15a increased Foxo1, IL-1β, NLRP3, and cleaved caspase 1 levels. REC grown in high glucose transfected with the miR15a mimic had decreased levels of Foxo1 and NLRP3. miR15a directly binds to Foxo1. miR15a regulates NLRP3 actions in the retinal vasculature. Work in mice showed that loss of miR15a increased NLRP3 pathway signaling and Foxo1. miR15a mimics decreased levels of Foxo1 and NLRP3. Taken together, miR15a reduced inflammasome proteins and Foxo1 levels in the retinal vasculature.

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“…Through the regulation of DLK1 level, miR-15a could disrupt adipogenic differentiation [ 12 ]. miR-15a can also target Foxo1, and participate in the disruption of adipogenic differentiation and the regulation of insulin synthesis [ 63 – 65 ]. miR-22 can regulate the PTEN/AKT pathway and target HDAC6 [ 66 – 68 ]; miR-206 and miR-1a can suppress hepatic lipogenesis [ 69 ]; miR-29b and miR-9 are involved in insulin sensitivity and diabetes [ 70 , 71 ]; miR-31 and miR-32 participate in differentiation of stem cells into adipocyte and lipid metabolism in oligodendrocytes [ 72 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Expression Profiling of Preadipocyte MicroRNAs by Deep Sequencing on Chicken Lines Divergently Selected for Abdominal Fatness

Wang

Cheng

et al. 2015

PLoS ONE

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Through posttranscriptional gene regulation, microRNA (miRNA) is linked to a wide variety of biological processes, including adipogenesis and lipid metabolism. Although miRNAs in mammalian adipogenesis have been worked on extensively, their study in chicken adipogenesis is still very limited. To find miRNAs potentially important for chicken preadipocyte development, we compared the preadipocyte miRNA expression profiles in two broiler lines divergently selected for abdominal fat content, by sequencing two small RNA libraries constructed for primary preadipocytes isolated from abdominal adipose tissues. After bioinformatics analyses, from chicken miRNAs deposited in miRBase 20.0, we identified 225 miRNAs to be expressed in preadipocytes, 185 in the lean line and 200 in the fat line (derived from 208 and 203 miRNA precursors, respectively), which corresponds to 114 miRNA families. The let-7 family miRNAs were the most abundant. Furthermore, we validated the sequencing results of 15 known miRNAs by qRT-PCR, and confirmed that the expression levels of most miRNAs correlated well with those of Solexa sequencing. A total of 33 miRNAs was significantly differentially expressed between the two chicken lines (P<0.05). Gene ontology analysis revealed that they could target genes enriched in the regulation of gene transcription and chromatin function, response to insulin stimulation, and IGF-1 signaling pathways, which could have important roles in preadipocyte development. Therefore, a valuable information and resource of miRNAs on chicken adipogenesis were provided in this study. Future functional investigations on these miRNAs could help explore related genes and molecular networks fundamental to preadipocyte development.

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MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1

Cited by 39 publications

References 32 publications

CircINSR regulates fetal bovine muscle and fat development

CircINSR regulates fetal bovine muscle and fat development

miR15a regulates NLRP3 inflammasome proteins in the retinal vasculature

Expression Profiling of Preadipocyte MicroRNAs by Deep Sequencing on Chicken Lines Divergently Selected for Abdominal Fatness

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