miR-154-5p Affects the TGFβ1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1

Bian, Che; Luan, Zhilin; Zhang, Haibo; Zhang, Ruijing; Gao, Jing; Wang, Yuxia; Li, Jia; Ren, Huiwen

doi:10.1155/2022/7502632

Cited by 6 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ubiquitination is a crucial biological process for protein turnover [ 5 , 6 ]. This process is achieved via the cooperation of the E1 enzyme, E2 enzyme, and E3 ligase [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Analysis Revealing FBXW9 as a Potential Prognostic and Immunological Biomarker in Breast Cancer

Liang

Fan

et al. 2023

IJMS

View full text Add to dashboard Cite

The WD40 repeat-containing F-box proteins (FBXWs) family belongs to three major classes of F-box proteins. Consistent with the function of other F-box proteins, FBXWs are E3 ubiquitin ligases to mediate protease-dependent protein degradation. However, the roles of several FBXWs remain elusive. In the present study, via integrative analysis of transcriptome profiles from The Cancer Genome Atlas (TCGA) datasets, we found that FBXW9 was upregulated in the majority of cancer types, including breast cancer. FBXW expression was correlated with the prognosis of patients with various types of cancers, especially for FBXW4, 5, 9, and 10. Moreover, FBXWs were associated with infiltration of immune cells, and expression of FBXW9 was associated with poor prognosis of patients receiving anti-PD1 therapy. We predicted several substrates of FBXW9, and TP53 was the hub gene in the list. Downregulation of FBXW9 increased the expression of p21, a target of TP53, in breast cancer cells. FBXW9 was also strongly correlated with cancer cell stemness, and genes correlated with FBXW9 were associated with several MYC activities according to gene enrichment analysis in breast cancer. Cell-based assays showed that silencing of FBXW9 inhibited cell proliferation and cell cycle progression in breast cancer cells. Our study highlights the potential role of FBXW9 as a biomarker and promising target for patients with breast cancer.

show abstract

“…Ubiquitination is a crucial biological process for protein turnover [ 5 , 6 ]. This process is achieved via the cooperation of the E1 enzyme, E2 enzyme, and E3 ligase [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Analysis Revealing FBXW9 as a Potential Prognostic and Immunological Biomarker in Breast Cancer

Liang

Fan

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Drugs derived from plants can ameliorate mesangial cell metabolism, thereby effectively treating DN [ 26 ]. Notably, miRNAs, signal transduction pathways, and mesangial cells are all interconnected with one another such that miRNAs can modulate key signaling pathways to modulate the metabolic activity of mesangial cells, thus affecting the development and severity of DN [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…There have been over 18,000 DN-related manuscripts published since the beginning of the 21 st century corresponding (1) Drug therapy of DN 58, 33,4,36,25,15,41,51,13,18,59 (1) Single-gene polymorphism of DN (2) Dialysis on DN 27,11,32,40,57,43,20,12,30,50,56,55 (1) Physiopathology of DN (2) Metabolism of DN 45,42,35,53,48,24,2,26,22,34,49,23,8,37 (1) Podocyte metabolism in DN (2) Signal transduction of DN (3) MicroRNAs of DN (4) Mesangial-cell metabolism in DN 9 Oxidative Medicine and Cellular Longevity to a diverse array of topics including DN-related drug therapy [24][25][26], the pathology and pathophysiology of DN [27], the diagnosis of DN [28,29], the etiology of DN [30,31], and the epidemiology of DN [32,…”

Section: Discussionmentioning

confidence: 99%

Bibliometric Analysis and Visualization of Research Progress in the Diabetic Nephropathy Field from 2001 to 2021

Shao

Shi

2023

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Background/Aims. Diabetic nephropathy (DN) remained the leading driver of global end-stage renal disease (ESRD) incidence, and tens of thousands of studies to date have analyzed DN-related topics. However, no bibliometric analysis of DN-associated studies indexed in the PubMed database from 2001 to 2021 has been conducted to date. The present bibliometric study was thus developed to visualize and offer insight into the knowledge framework, research hotspots, and dynamic changes in the DN field over the course from 2001 to 2021. Methods. The PubMed database was searched to identify all studies related to DN that were published from 2001 to 2021, with these studies being separated into four time-based groups. The characteristics of these studies were analyzed and extracted using BICOMB. Biclustering analyses for each of these groups were then performed using gCLUTO, with these results then being analyzed and GraphPad Prism 5 being used to construct strategy diagrams. The social network analyses (SNAs) for each group of studies were conducted using NetDraw and UCINET. Results. In total, 18,889 DN-associated studies published from 2001 to 2021 and included in the PubMed database were incorporated into the present bibliometric analysis. Biclustering analysis and strategy diagrams revealed that active areas of research interest in the DN field include studies of the drug-based treatment, diagnosis, etiology, pathology, physiopathology, and epidemiology of DN. The specific research topics associated with these individual areas, however, have evolved over time in a dynamic manner. Strategy diagrams and SNA results revealed podocyte metabolism as an emerging research hotspot in the DN research field from 2010 to 2015, while DN-related microRNAs, signal transduction, and mesangial cell metabolism have emerged as more recent research hotspots in the interval from 2016 to 2021. Conclusion. Through analyses of PubMed-indexed studies pertaining to DN published since 2001, the results of this bibliometric analysis offer a knowledge framework and insight into active and historical research hotspots in the DN research space, enabling investigators to readily understand the dynamic evolution of this field over the past two decades. Importantly, these analyses also enable the prediction of future DN-related research hotspots, thereby potentially guiding more focused and impactful research efforts.

show abstract

“…MiRNAs have been shown to influence disease development by affecting downstream targets 34 35 . In our study, we verified miR-545-3p targeted USP15 and reversed its expression.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circADAM9 Promotes Inflammation, Oxidative Stress, and Fibrosis of Human Mesangial Cells via the Keap1-Nrf2 Pathway in Diabetic Nephropathy

Zheng

Liu

Song

2023

Exp Clin Endocrinol Diabetes

View full text Add to dashboard Cite

Objective Circular RNAs (circRNAs) have been discovered as potential biomarkers for diabetic nephropathy (DN). In this study, the potential roles of circADAM9 in high glucose (HG)-induced cell injury of human mesangial cells (HMCs) were investigated, and the underlying mechanism was elucidated. Methods DN cell model in vitro was simulated by HG treatment of HMCs. Endogenous expressions of circADAM9, miR-545-3p, and ubiquitin-specific protease 15 (USP15) were determined by real-time polymerase chain reaction. Cell proliferation and migration were evaluated using Cell Counting Kit-8 and wound healing assays. The inflammatory response was assessed by enzyme-linked immunosorbent assay. Oxidative stress was examined using commercially available kits. Dual-luciferase reporter and RNA pull-down assays were conducted to confirm the interaction among circADAM9, miR-545-3p, and USP15. Results CircADAM9 was upregulated in DN samples and HG-treated HMCs, while its downregulation inhibited cell proliferation, inflammation, fibrosis, and oxidative stress. Further investigation revealed that circADAM9 exerted this influence by targeting the miR-545-3p/USP15 axis, thereby regulating the KELCH-like ECh-associated protein 1/nuclear factor erythroid 2 related factor 2 (Keap1/Nrf2) pathway. MiR-545-3p knockdown or USP15 overexpression reversed the effect of circADAM9 silencing in HG-induced HMCs. Conclusion These results indicate that the circADAM9/miR-545-3p/USP15/Keap1/Nrf2 signaling axis is critical for HG-induced cell injury in HMCs and might represent a novel therapeutic target for DN treatment.

show abstract

miR-154-5p Affects the TGFβ1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1

Cited by 6 publications

References 44 publications

A Comprehensive Analysis Revealing FBXW9 as a Potential Prognostic and Immunological Biomarker in Breast Cancer

A Comprehensive Analysis Revealing FBXW9 as a Potential Prognostic and Immunological Biomarker in Breast Cancer

Bibliometric Analysis and Visualization of Research Progress in the Diabetic Nephropathy Field from 2001 to 2021

Circular RNA circADAM9 Promotes Inflammation, Oxidative Stress, and Fibrosis of Human Mesangial Cells via the Keap1-Nrf2 Pathway in Diabetic Nephropathy

Contact Info

Product

Resources

About