miR-153 Silencing Induces Apoptosis in the MDA-MB-231 Breast Cancer Cell Line

Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-153 inhibition in the breast carcinoma cell line MDA-MB-231. Forty-eight hours after MDA-MB-231 cells were transfected with the miR-153 inhibitor, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects of miR-153 on cell viability. Flow cytometry analysis and assessment of caspase 3/7 activity were adopted to determine whether miR-153 affects the proliferation rates and apoptosis levels of MDA-MB-231 cells. Our results showed that silencing of miR-153 significantly inhibited growth when compared to controls at 48 hours, reducing proliferation by 37.6%, and inducing apoptosis. Further studies are necessary to corroborate our findings and examine the potential use of this microRNA in future diagnostic and therapeutic interventions.

Down-regulation of miRNA-452 is Associated with Adriamycin-resistance in Breast Cancer Cells

“…Recently dysregulated miRNAs have been reported that associated with every aspect of breast tumor biology, including tumor progression (Li et al, 2013a), apoptosis (Anaya-Ruiz et al, 2013), different distribution in clinicopathological subtypes (Li et al, 2013b), and acquisition of resistance to various chemotherapeutic agents. Causes of anti-cancer drug resistance are believed to be complex and current studies have indicated that the acquisition of chemotherapy drug resistance may also be modulated via the changes in miRNA levels.…”

MicroRNA‐153‐3p regulates cell proliferation and cisplatin resistance via Nrf‐2 in esophageal squamous cell carcinoma

“…2 Some studies have found that miR-153-3p can inhibit the proliferation and invasive growth of breast cancer and osteosarcoma cells. 9,10 These findings indicate that miR-153-3p can act as a tumor suppressor and may serve as a potential target for the treatment of malignant tumors. However, whether miR-153-3p regulates the proliferation of ESCC cells and confers sensitivity to cisplatin chemotherapy remains unclear.…”

“…Upregulation of miR‐153 has been shown to inhibit the migration and invasion of ESCC cells, both in vitro and in vivo . Some studies have found that miR‐153‐3p can inhibit the proliferation and invasive growth of breast cancer and osteosarcoma cells . These findings indicate that miR‐153‐3p can act as a tumor suppressor and may serve as a potential target for the treatment of malignant tumors.…”

Retracted: Bilobalide alleviates tumor necrosis factor‐alpha‐induced pancreatic beta‐cell MIN6 apoptosis and dysfunction through upregulation of miR‐153

“…A recent research reported that miR‐153 functioned as a negative regulator of insulin secretion in MIN6 cells (Xu et al, ). Another report identified that apoptosis of breast cancer cells were induced by silencing miR‐153 (Anaya‐Ruiz, Cebada, Delgado‐Lopez, Sanchez‐Vazquez, & Perez‐Santos, ). According to the above researcher's literatures, we aimed to identify the relationship between BB and miR‐153 in MIN6 cells.…”