miR‑151a‑5p promotes the proliferation and metastasis of colorectal carcinoma cells by targeting AGMAT

Abstract

Colorectal carcinoma (CRC) is one of the most common types of digestive cancer. It has been reported that the ectopic expression of microRNAs (miRs) plays a critical role in the occurrence and progression of CRC. In addition, it has also been suggested that miR-151a-5p may serve as a useful biomarker for the early detection and treatment of different types of cancer and particularly CRC. However, the specific effects and underlying mechanisms of miR-151a-5p in CRC remain elusive. The results of the current study demonstrated that miR-151a-5p was upregulated in CRC cell lines and clinical tissues derived from patients with CRC. Functionally, the results showed that miR-151a-5p significantly promoted CRC cell proliferation, migration and invasion. Additionally, dual luciferase reporter assays verified that agmatinase (AGMAT) was a direct target of miR-151a-5p and it was positively associated with miR-151a-5p expression. Mechanistically, miR-151a-5p could enhance the epithelial-mesenchymal transition of CRC cells. Taken together, the results of the current study revealed a novel molecular mechanism indicating that the miR-151a-5p/AGMAT axis could serve a crucial role in the regulation of CRC and could therefore be considered as a potential therapeutic strategy for CRC.

Catalytic Relaxation of Kinetically Trapped Intermediates by DNA Chaperones

“…With addition of a DNA oligo serving as a catalyst (see “target/chaperone/c-151” in Table S1 for the sequence), we expect that a thermodynamically more stable hairpin HP3 will form after a toehold-mediated strand displacement and self-assembly (Figure A). , The catalyst or chaperone employed in this study serves as a DNA substitute for microRNA-151, a clinically significant molecule recognized as a crucial cancer biomarker. Research indicates that miRNA-151 plays a pivotal role in the proliferation and metastasis of various cancer types. , As expected, in the stretching FX curves (blue curve in Figure B), only one rupture event was observed at an unfolding force (14.2 ± 2.2 pN) greater than that of each of the two small hairpins. The Δ L of the feature was consistent with that of the HP3 (observed Δ L, 38.0 ± 3.7 nm; expected Δ L for HP3: 37.6 nm; see Figure S7 for calculation).…”

“…24,25 151 plays a pivotal role in the proliferation and metastasis of various cancer types. 28,29 As expected, in the stretching FX curves (blue curve in Figure 1B), only one rupture event was observed at an unfolding force (14.2 ± 2.2 pN) greater than that of each of the two small hairpins. The ΔL of the feature was consistent with that of the HP3 (observed ΔL, 38.0 ± 3.7 nm; expected ΔL for HP3: 37.6 nm; see Figure S7 for calculation).…”

microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG

“…Many microRNAs (miRNAs and miRs) are associated with EMT in normal and cancer cells (6)(7)(8). However, only a few studies on the association between miRNAs and EMT have been published in veterinary medicine (9)(10)(11).…”

Cross-Kingdom Interaction of miRNAs and Gut Microbiota with Non-Invasive Diagnostic and Therapeutic Implications in Colorectal Cancer

“…The following supporting information can be downloaded at: . References [ 19 , 20 , 26 , 27 , 43 , 48 , 49 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 , 170 , 171 , 172 , 173 , 174 , 175 , 176 , 177 , 178 , 179 , 180 , 181 , 182 , 183 , 184 , 185 , 186 , 187 , 188 , 189 , 190 , 191 , 192 , 193 , 194 , 195 , 196 , 197 , 198 , 199 , 200 , 201 , 202 , 203 , 204 , 205 , 206 , 207 , 208 , 209 , 210 <...…”