miR-150 Suppresses Tumor Growth in Melanoma Through Downregulation of MYB

Sun, Xiyan; Zhang, Chao; Cao, Yang; Liu, Erbiao

doi:10.3727/096504018x15228863026239

Cited by 26 publications

(24 citation statements)

References 31 publications

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“…In contrast, high levels of the mir-150 was associated with good prognosis in three tumor types and, in G2 melanoma samples, it was highly correlated with the presence of CD8+ T cells. Consistent with our findings, this miRNA was previously reported to be highly expressed in B and T cells [51] and higher levels of mir-150 were found to inhibit proliferation, migration, and invasion of melanoma cells [52]. In accordance with our findings, mir-150 has also been associated with better patient's survival [52] and suggested as a potential metastatic melanoma biomarker [53], while the expression of one of its target in malignant cells, HILPDA, was shown to be induced by hypoxia and associated with tumor resistance to antiangiogenesis treatments [54].…”

Section: Discussionsupporting

confidence: 92%

Poor clinical outcome in metastatic melanoma is associated with a microRNA-modulated immunosuppressive tumor microenvironment

et al. 2020

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Background: Interaction between malignant cells and immune cells that reside within the tumor microenvironment (TME) modulate different aspects of tumor development and progression. Recent works showed the importance of miRNA-containing extracellular vesicles in this crosstalk. Methods: Interested in understanding the interplay between melanoma and immune-related TME cells, we characterized the TCGA's metastatic melanoma samples according to their tumor microenvironment profiles, HLA-I neoepitopes, transcriptome profile and classified them into three groups. Moreover, we combined our results with melanoma single-cell gene expression and public miRNA data to better characterize the regulatory network of circulating miRNAs and their targets related to immune evasion and microenvironment response. Results: The group associated with a worse prognosis showed phenotypic characteristics that favor immune evasion, including a strong signature of suppressor cells and less stable neoantigen:HLA-I complexes. Conversely, the group with better prognosis was marked by enrichment in lymphocyte and MHC signatures. By analyzing publicly available melanoma single-cell RNA and microvesicle microRNAs sequencing data we identified circulating micro-RNAs potentially involved in the crosstalk between tumor and TME cells. Candidate miRNA/target gene pairs with previously reported roles in tumor progression and immune escape mechanisms were further investigated and demonstrated to impact patient's overall survival not only in melanoma but across different tumor types. Conclusion: Our results underscore the impact of tumor-microenvironment interactions on disease outcomes and reveal potential non-invasive biomarkers of prognosis and treatment response.

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Section: Discussionsupporting

confidence: 92%

Poor clinical outcome in metastatic melanoma is associated with a microRNA-modulated immunosuppressive tumor microenvironment

et al. 2020

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“…GC accounts for 10% of all diagnosed cancer types each year and accounts for 12% of all cancer associated mortalities worldwide (2,4). It is also one of the most common malignancies (13,14). The current study assessed the role and mechanism of miR-150-SRCIN1 in the pathogenesis of GC development.…”

Section: Discussionmentioning

confidence: 99%

“…miRNA has been demonstrated to be involved in the regulation of tumor cell proliferation, apoptosis, differentiation, drug resistance, invasion and metastasis, among which miR-150 also ion, drug resistance, invasion and metastasis, among which miR-150 also serves an important role in the development of tumors (10)(11)(12)(13)(14)(15). In colorectal cancer, breast cancer and melanoma, miR-150 is significantly downregulated and exerts a tumor suppressive role (11)(12)(13). However, in cervical cancer, miR-150 is highly expressed and promotes tumor progression (14).…”

Section: Introductionmentioning

confidence: 99%

“…However, in cervical cancer, miR-150 is highly expressed and promotes tumor progression (14). Therefore, miR-150 serves different roles in different tumor types (10)(11)(12)(13)(14). miR-150-5p, a member of the miR-150 family, has been determined to inhibit cancer cell aggressiveness by targeting cwcv and kazal like domains proteoglycan 1 in head and neck squamous cell carcinoma (15).…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA‑150‑5p and SRC kinase signaling inhibitor 1 involvement in the pathological development of gastric cancer

Quan

Chen

et al. 2019

Exp Ther Med

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The current study aimed to assess the regulatory mechanism of microRNA-150-5p (miR-150-5p) in the pathogenesis of gastric cancer. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to verify the expression of miR-150-5p in gastric cancer tissues and cell lines, which was revealed to be highly expressed in each. In addition, the expression of miR-150-5p was associated with advanced gastric cancer and lymph node metastasis. The current study then hypothesized that SRC kinase signaling inhibitor 1 (SRCIN1) was the target gene of miR-150-5p, a theory that was confirmed via a dual luciferase reporter gene assay. RT-qPCR and western blotting were then performed to verify the expression of SRCIN1 in gastric cancer tissues and cell lines. The results demonstrated that SRCIN1 was lowly expressed in gastric cancer tissues and cells. To assess the effect of miR-150-5p on gastric cancer cells, experiments were conducted with BGC-823 cells transfected with a miR-150-5p inhibitor or a miR-150-5p inhibitor+SRCIN1-small interfering (si)RNA respectively. A cell counting kit-8 assay and flow cytometry were also used to assess cell viability and apoptosis, respectively. Western blotting and RT-qPCR were further used to measure the expression of specific markers of epithelial mesenchymal transition (EMT), including epithelial cell markers (E-cadherin and zona occluding-1) and interstitial cell markers (vimentin, N-cadherin and β-catenin). The results revealed that the miR-150-5p inhibitor attenuated cell viability, induced apoptosis, decreased the expression of interstitial cell markers and increased epithelial cell marker expression. However, all effects of the miR-150-5p inhibitor were reversed following SRCIN1-siRNA treatment. In summary, the current study indicated that the miR-150-5p inhibitor attenuated cell viability, induced apoptosis and inhibited gastric cancer cell EMT by targeting SRCIN1.

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“…Transcriptomic classification of the immune cluster was achieved by the quantification of CD2 and PDL1 mRNAs, while for keratin and MITF low clusters, the expression level of three pigmentation genes was analyzed, namely MITF , TYRP1 , and TRPM1 because of their different expression in “keratin” and “MITF-low” groups [ 5 ]. Furthermore, four microRNAs were also investigated for their possible association with clinical features, prognosis and survival [ 7 , 8 , 11 , 12 , 13 , 14 , 15 ], and transcriptomic analyses. In detail, miR-150-5p was linked with the tumor-infiltrated lymphocytes (TILSs) and with the proto-oncogene MYB, which plays a role in the regulation of hematopoiesis and tumorigenesis [ 8 , 11 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

The Association of Residential Altitude on the Molecular Profile and Survival of Melanoma: Results of an Interreg Study

Martino

Brunetti

Canzonieri

et al. 2020

Cancers

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Cutaneous melanoma (CM) incidence is rising worldwide and is the primary cause of death from skin disease in the Western world. Personal risk factors linked to environmental ultraviolet radiation (UVR) are well-known etiological factors contributing to its development. Nevertheless, UVR can contribute to the development of CM in different patterns and to varying degrees. The present study aimed at investigating whether altitude of residence can contribute to the development of specific types of CM and/or influence its progression. To this aim, 306 formalin-fixed and paraffin-embedded (FFPE) tissues from primary CM diagnosed in different geographical areas were submitted to B-RAF proto-oncogene serine/threonine kinase (BRAF) and N-RAS proto-oncogene GTPase (NRAS) mutational status detection and mRNA and miRNA profiling by qPCR. Genes were chosen for their functions in specific processes, such as immune response (CD2, PDL1, or CD274) and pigmentation (MITF, TYRP1, and TRPM1). Furthermore, four microRNAs, namely miR-150-5p, miR-155-5p, miR-204-5p, and miR-211-5p, were included in the profiling. Our results highlight differences in the gene expression profile of primary CM with respect to the geographical area and the altitude of residence. Melanoma-specific survival was influenced by the gene expression of mRNA and miRNAs and varied with the altitude of patients’ residence. In detail, TYRP1 and miR-204-5p were highly expressed in patients living at higher altitudes, unlike miR-150-5p, miR-155-5p, and miR-211-5p. Since miRNAs are highly regulated by reactive oxygen species, it is possible that different regulatory mechanisms characterize CMs at different altitudes due to the different environment and UVR intensity.

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miR-150 Suppresses Tumor Growth in Melanoma Through Downregulation of MYB

Cited by 26 publications

References 31 publications

Poor clinical outcome in metastatic melanoma is associated with a microRNA-modulated immunosuppressive tumor microenvironment

Poor clinical outcome in metastatic melanoma is associated with a microRNA-modulated immunosuppressive tumor microenvironment

MicroRNA‑150‑5p and SRC kinase signaling inhibitor 1 involvement in the pathological development of gastric cancer

The Association of Residential Altitude on the Molecular Profile and Survival of Melanoma: Results of an Interreg Study

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