miR‐148‐3p and miR‐152‐3p synergistically regulate prostate cancer progression via repressing KLF4

Feng, Feng; Liu, Hui; Chen, Aiping; Xia, Qinghua; Zhao, Yong; Jin, Xunbo; Huang, Jianjun

doi:10.1002/jcb.28984

Cited by 46 publications

(37 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intriguingly, OLA had the ability to reverse IL‐1β‐induced down‐regulation of miR‐148‐3p. Published studies have reported that the functions of miR‐148‐3p are focused on antineoplastic activity . The present study is the first to indicate that miR‐148‐3p might be involved in chondrocyte dysfunction.…”

Section: Discussionsupporting

confidence: 56%

Oleanolic acid mitigates interleukin‐1β‐induced chondrocyte dysfunction by regulating miR‐148‐3p‐modulated FGF2 expression

Nie

Ping

2020

The Journal of Gene Medicine

View full text Add to dashboard Cite

Background: microRNA (miR)-mediated post-transcriptional repression has been reported in the process of chondrocyte dysfunction. The present study aimed to investigate the molecular mechanisms underlying in oleanolic acid (OLA)-prevented interleukin (IL)-1β-induced chondrocyte dysfunction via the miR-148-3p/fibroblast growth factor-2 (FGF-2) signaling pathway.Methods: Candidate miRs were filtrated using miR microarray assays in chondrocytes with or without IL-1β stimulation. Gene expression of candidate miRs and protein expression of FGF2 were analyzed using a quantitative reverse transcriptasepolymerase chain reaction and western blotting, respectively. Cell growth was evaluated using cell counting kit-8 assays. Cell apoptosis was detected using Annexin V-fluorescein isothiocyanate double staining.Results: Treatment with OLA counteracted IL-1β-evoked chondrocyte growth inhibition, apoptosis, caspase3 production, and release of malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine. Additionally, FGF2 protein expression levels elevated by IL-1β were down-regulated by OLA and transfection with miR-148-3p mimics. IL-1β-induced down-regulation of miR-148-3p in chondrocytes was evaluated by OLA administration. Bioinformatics algorithms and experimental measurements indicated that FGF2 might be a direct target of miR-148-3p. miR-148-3p mimics exhibited equal authenticity of OLA to protect against IL-1β-induced chondrocyte dysfunction. Conclusions:Our present findings highlight a protective effect of OLA on IL-1βinduced chondrocyte dysfunction, and a novel signal cascade comprising the miR-148-3p/FGF2 signaling pathway might be a potential therapeutic target of OLA with respect to preventing the progression of osteoarthritis.

show abstract

Section: Discussionsupporting

confidence: 56%

Oleanolic acid mitigates interleukin‐1β‐induced chondrocyte dysfunction by regulating miR‐148‐3p‐modulated FGF2 expression

Nie

Ping

2020

The Journal of Gene Medicine

View full text Add to dashboard Cite

show abstract

“…Some studies have revealed that miR-148 and miR-10 inhibited the proliferation of different types of cells. For example, miR-148-3p inhibited the growth of glioblastoma by targeting DNMT1 and PCa cell growth in vivo [44,45]. It was also found that miR-148a could suppress human renal cell carcinoma malignancy by targeting AKT2 [46].…”

Section: Discussionmentioning

confidence: 97%

Preliminary study on microR-148a and microR-10a in dermal papilla cells of Hu sheep

Gao

Jin

et al. 2019

BMC Genet

View full text Add to dashboard Cite

Background: Hu sheep, a unique Chinese breed with high reproductive performance, are also well known for their rare white lambskin in China. The quality of lambskin is affected by hair follicles, and dermal papilla cells are an important component of hair follicles that plays a key role in hair follicle growth and development. This study helps elucidate the effect of miR-148a and miR-10a on hair follicle growth and development. Results: Based on the results of gene chip and high-throughput sequencing, bone morphogenetic protein 7 (BMP7) was used as a research object. Bioinformatics analysis and the dual-luciferase reporter system indicated that, along with Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) that miR-148a and miR-10a target relationships with BMP7. BMP7 was the target gene both for miR-148a and miR-10a by the dual-luciferase reporter system and Western blot. Hu sheep dermal papilla cells were successfully isolated and purified, and after transfecting miR-148a/miR-10a mimics and inhibitors into dermal papilla cells, a Cell Counting Kit-8 (CCK-8) was used to determine that miR-148a/miR-10a inhibited the proliferation of Hu sheep dermal papilla cells. In addition, after the overexpression of miR-148a, the expression levels of Smad3 (P < 0.05), Smad6 (P < 0.05), Smad4 (P < 0.01), and Smad5 (P < 0.01) were significantly higher than those of the control groups. After the inhibition of miR-148a, the expression levels of Smad3 (P < 0.05), Smad4 (P < 0.05), and TGF-β (P < 0.01) were significantly lower than those of the control groups. After the overexpression of miR-10a, the expression levels of Smad1 (P < 0.01), Smad2 (P < 0.05), Smad4 (P < 0.01), Smad5 (P < 0.01), and TGF-β (P < 0.05) were significantly lower than those of the control groups. After the inhibition of miR-10a, the expression levels of Smad1 (P < 0.01) and Smad2 (P < 0.05) were significantly lower than those of the control groups. Conclusions: These results revealed the target relationship between miR-148a, miR-10a and BMP7, and the effect of miR-148a and miR-10a on the proliferation of dermal papilla cells. They will provide the basis for a follow-up study on how miR-148a, and miR-10a mediate BMP7 regulation of hair follicle growth and development.

show abstract

“…Interestingly, the protein expression of RHOA was significantly negatively correlated with miR-154-3p and miR-487-3p expression in 63 thyroid cancer tissues. miRs play crucial roles in modulating gene expression via post-transcriptional repression [25,26]. In fact, single miR can modulate multiple target genes, in contrast with that, one gene can be regulated by multiple miRs [25,26].…”

Section: Discussionmentioning

confidence: 99%

“…miRs play crucial roles in modulating gene expression via post-transcriptional repression [25,26]. In fact, single miR can modulate multiple target genes, in contrast with that, one gene can be regulated by multiple miRs [25,26]. For example, miR-143 and miR-145 gain-of-function synergistically suppress cell proliferation and invasion in breast cancer through the repression of ERBB3 protein expression [25].…”

Section: Discussionmentioning

confidence: 99%

“…For example, miR-143 and miR-145 gain-of-function synergistically suppress cell proliferation and invasion in breast cancer through the repression of ERBB3 protein expression [25]. miR-148-3p and miR-152-3p realize synergistic effect to inhibit cell proliferation and induce apoptosis in prostate cancer cells [26]. In thyroid cancer, we discovered a collaborative mechanism of miRs that miR-154-3p and miR-487-3p tend to function as tumor suppressors in human thyroid cancer via post-transcriptional repression of RHOA protein expression, but not mRNA levels.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

miR-154-3p and miR-487-3p synergistically modulate RHOA signaling in the carcinogenesis of thyroid cancer

et al. 2020

View full text Add to dashboard Cite

Correspondence: Ning An (anning gpch@tom.com) Background: miRs family members are often thought to have extensively overlapping targets and synergistically to modulate target gene expression via post-transcriptional repression. The present study was to determine whether miR-154-3p and miR-487-3p synergistically collaborated to regulate RHOA signaling in the carcinogenesis of thyroid cancer. Materials and methods: Candidate miRs were filtrated using miR microarray assays. Gene and protein expression levels were analyzed using RT-qPCR and Western blotting, respectively. Cell growth was evaluated using CCK8 assays and nude-mouse transplanted tumor experiments. Cell apoptosis was detected using Annexin V-FITC double staining. Results: miR-154-3p and miR-487-3p were significantly decreased in 63 thyroid cancer tissues and cell lines compared with those in paired non-tumor tissues and normal thyroid follicular epithelial cells. Low expression levels of miR-154-3p and miR-487-3p significantly correlated with tumor size, TNM stage, histological grade, lymph node metastasis and shorter overall survival in patients with thyroid cancer. Furthermore, the protein expression of RHOA was significantly inversely correlated with miR-154-3p (r = −0.404; P = 0.001) and miR-487-3p (r = −0.456; P < 0.001) expression in thyroid cancer tissues. We experimentally validated that miR-154-3p and miR-487-3p synergistically blocked thyroid cancer cell growth in vitro and in vivo. However, the anti-proliferative and pro-apoptotic activities of miR-154-3p/487-3p were neutralized by RHOA overexpressed vectors. Conclusions: Our present findings expounded a novel signal cascade employing miR-154-3p/487-3p and RHOA to fine-tune thyroid cancer cell proliferation and apoptosis. We corroborated that suppression of RHOA by miR-154-3p/487-3p may be a valuable therapeutic target for impeding thyroid cancer progression.Ras homolog family member A (RHOA) as a representative protein of RHO subfamily primarily participates in actin cytoskeleton rearrangement, which is implicated in various physiological functions, including cell proliferation, differentiation, migration, polarity and gene transcription [6]. Rho-associated protein kinase (ROCK) is a downstream effector of RHOA and has the ability to modulate cytoskeleton dynamics [6]. Over-activation of RHOA/ROCK signaling pathway may be associated with a decrease in cell-cell junctions and a promotion of cell motility [7]. Previous studies have validated that high expression of RHOA and ROCK is frequently reported in various cancer-types, such as breast cancer, ovarian cancer and colon cancer [6,8,9]. In thyroid cancer, RHOA/ROCK signaling has a profound stimulative impact to facilitate the invasiveness of anaplastic thyroid cancer cells [10]. microRNAs (miRs) are a class of non-coding RNAs and have widely been elaborated in the pathogenesis of various diseases, including carcinogenesis [11]. miRs function as post-transcriptional regulators to repress target genes translation via binding with their 3 -un...

show abstract

miR‐148‐3p and miR‐152‐3p synergistically regulate prostate cancer progression via repressing KLF4

Cited by 46 publications

References 36 publications

Oleanolic acid mitigates interleukin‐1β‐induced chondrocyte dysfunction by regulating miR‐148‐3p‐modulated FGF2 expression

Oleanolic acid mitigates interleukin‐1β‐induced chondrocyte dysfunction by regulating miR‐148‐3p‐modulated FGF2 expression

Preliminary study on microR-148a and microR-10a in dermal papilla cells of Hu sheep

miR-154-3p and miR-487-3p synergistically modulate RHOA signaling in the carcinogenesis of thyroid cancer

Contact Info

Product

Resources

About