MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl

Wang, Shuo; Tang, Weicheng; Ma, Liyuan; Yang, Jun; Huang, Kecheng; Du, Xiaofang; Luo, Aiyue; Shen, Wei; Ding, Ting; Ye, Shuangmei; Zhou, Shanshan; Yang, Shuhong; Wang, Shixuan

doi:10.1016/j.lfs.2021.119820

Cited by 6 publications

(10 citation statements)

References 37 publications

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“…36,37 Nevertheless, the effect of miR-145 may be cell/ tissue-specific, as it could promote the proliferation, differentiation, and steroidogenesis of ovarian granulosa cells. 38 In bovine primary myoblasts, the sponge of miR-145 activated IGF1/PI3K/AKT signaling, promoting muscle proliferation and differentiation, as well as suppressing muscle apoptosis. 39 Nonetheless, to the best of our knowledge, no data exist on the role of miR-145-5p in the growth and differentiation of GPMs.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13

et al. 2022

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MicroRNAs (miRNAs) are evolutionarily conserved endogenous small non‐coding RNAs that play critical roles in skeletal muscle development. In this study, we identified putative miRNAs that were differentially expressed in the longissimus dorsi muscle between fetus (75 days of pregnancy) and lamb (1 day of age). We detected 1208 miRNAs, 313 of which were differentially expressed. In particular, we found that miR‐145‐5p was differentially and highly expressed in lamb skeletal muscle. In addition, our results demonstrated that overexpression of miR‐145‐5p inhibited the differentiation and apoptosis of goat primary myoblasts (GPMs), whereas knockdown of miR‐145‐5p had the opposite effect. The coding domain sequence (CDS) of ubiquitin‐specific peptidase 13 (USP13) was predicted and validated as a target of miR‐145‐5p. We also demonstrated that the influence as a key regulator of GPMs differentiation is primarily mediated by targeting and inhibiting USP13. Taken together, these results revealed a novel pathway in skeletal muscle development in which miR‐145‐5p targets CDS region of USP13 to regulate differentiation and apoptosis of GPMs.

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Section: Discussionmentioning

confidence: 99%

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13

et al. 2022

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“…miR-145 supports proliferation, differentiation, and steroidogenesis of granulosa cells and plays an important role in ovarian physiology and pathology. It is involved in the formation of the primordial follicle pool in fetal and neonatal mouse ovarian tissues (35,36).…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Oral Contraceptives On Ovarian Reserve, Histology, and Micrornas: An Experimental Study

Aslan¹,

Yavuzkır²,

Atılgan³

et al. 2022

targetmedj

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The aim of the present study was to investigate the effects of different oral contraceptives containing androgenic and antiandrogenic progestins on ovarian follicle reserve, germinal epithelial fibrosis, and microRNA (miRNA) expressions in the ovary. Materials and Methods:In this prospective study, 35 4-month-old adult female Wistar Albino rats weighing 190-220g and having a regular cycle were randomly divided into five groups during the estrus phase in a single-blind manner. Placebo tablets and oral contraceptives were delivered via gastric lavage for 3 months. Group 1 (control group) received placebo tablets, group 2 received ethinylestradiol (EE) 1.5mcg/day +drospirenone 150mcg/day (Yasmin®), group 3 received EE 1.5mcg/ day +cyproterone acetate 100mcg/day (Diane 35®), group 4 received EE 1.5mcg/ day +gestodene 3.75mcg/day (Ginera®), and group 5 received EE 1.5mcg/day + levonorgestrel 7.5mcg/day (Microgynon®). Bilateral oophorectomy was performed after 3months. The right ovary was used for the histological examination of the ovarian follicle pool (primordial, primary, secondary, tertiary follicle, fibrosis, corpus luteum [CL], and intra-CL angiogenesis) and surface epithelial change (germinal epithelial degeneration), and the left ovary was used to conduct genetic analysis of miRNAs (mir-21, mir-494, mir-191, and mir 145). Antimullerian hormone (AMH) was measured from intracardiac blood samples. For statistical analysis, the Kruskal-Wallis test was performed, followed by a pairwise comparison with the post-hoc Dunn's test, and p values of less than 0.05 were considered statistically significant. Results: When compared with Group 1, no significant difference was observed in blood AMH levels in Group 2, but blood AMH levels were found to be significantly decreased in groups 3, 4, and 5. Light microscopy revealed that the number of primordial follicles was similar across the groups, while Group 4 had a significantly lower number of primary, secondary, and tertiary follicles than the control and other experimental groups. Fibrosis and germinal epithelial degeneration were significantly increased in all combined oral contraceptive (COC) groups compared to the control group. mir-21, mir-494, mir-191, and mir-145 expression levels were found to be significantly higher in all COC groups than in the control group. Conclusion: Different progestin-containing COCs may affect ovarian reserve tests at different levels. are probably upregulated through the estrogen and progesterone receptor, due to the estrogen and progesterone containing oral contraceptives.

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“…MiR-145 and Arpc5 expression was downregulated or upregulated with miR-145 antagomirs (AT) or the corresponding negative controls (AN) (RiboBio, Guangzhou, China), respectively, or pEGFP-N1- Arpc5 /pEGFP-N1 vector, respectively, as previously described [ 15 ]. The GCs were cultured in 96-well plates at 37°C with 5% CO 2 in air for 24, 48, 72, and 96 h. Cells cultured in 6- and 24-well plates were harvested 48 h after transfection for quantitative real-time PCR (qRT-PCR), western blotting, and immunofluorescence assays.…”

Section: Methodsmentioning

confidence: 99%

“…The targets were then collected and Gene Ontology (GO) was performed for functional enrichment using the DAVID gene annotation tool (version 6.8) ( http://david.abcc.ncifcrf.gov/ ) [ 25 ]. We have already analyzed the target gene-enriched pathways previously [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…We also found that miR-145 targets Tgfbr2 to regulate the initiation of primordial follicle development and maintain primordial follicle quiescence [ 14 ]. Furthermore, we found that miR-145 targets Crkl and promotes the differentiation, proliferation, and steroidogenesis of GCs via the JNK-p38 MAPK signaling pathway [ 15 ] . In a previous study, a bioinformatics analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to analyze the enriched pathways of the miR-145 target genes included three pathways, axon guidance, adherens junction, and actin cytoskeletal regulation, which are all closely related to cell morphology changes, in the 10 most significantly enriched pathways [ 15 ].…”

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confidence: 99%

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<i>MiR-145</i> regulates steroidogenesis in mouse primary granulosa cells by targeting <i>Arpc5</i> and subsequent cytoskeleton remodeling

MA¹,

Wang

Yang

et al. 2023

J. Reprod. Dev.

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MicroRNA (miR)-145 is enriched in the follicular granulosa cells (GCs) of 3-week-old mice. Downregulating miR-145 inhibits the proliferation and differentiation of GCs and induces evident changes in their cytoskeleton. In this study, we examined how miR-145 induces cytoskeletal changes in mouse GCs and its potential mechanism in regulating GC steroidogenesis. We found that actin related protein 2/3 complex subunit 5 (Arpc5) is a target of miR-145. The miR-145 antagomir increased ARPC5 expression but not β-ACTIN, β-TUBULIN, and PAXILLIN expression. Arpc5 overexpression inhibited GC proliferation, differentiation, and progesterone synthesis. Furthermore, the expression of progesterone synthesis-associated enzymes was downregulated in the Arpc5 overexpression group, and the GC cytoskeleton exhibited evident changes. We conclude that Arpc5, a new target of miR-145, regulates primary GC proliferation and progesterone production by regulating the cytoskeleton remodeling.

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MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl

Cited by 6 publications

References 37 publications

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13

Effects of Different Oral Contraceptives On Ovarian Reserve, Histology, and Micrornas: An Experimental Study

<i>MiR-145</i> regulates steroidogenesis in mouse primary granulosa cells by targeting <i>Arpc5</i> and subsequent cytoskeleton remodeling

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