MiR‐145 inhibits the migration and invasion of papillary thyroid carcinoma cells through NF‐κB pathway regulation

Chen, Gengyu; Gao, Ying; Wang, Gang-Pu; Dai, Guofeng; Li, Tong

doi:10.1002/jcb.29604

Cited by 13 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In tumors, miRs can either function as tumor suppressor gene or oncogene by regulating downstream targets. It is reported that miR-145 was down-regulated in nasopharyngeal carcinoma [26], papillary thyroid carcinoma [27] and head and neck cancers [28]. For example, Liu et al [26] reported that miR-145 directed with CASC9, and the inhibition of miR-145 promoted cell migration and invasion but inhibited cell apoptosis in nasopharyngeal carcinoma cells, which provided a novel therapy for the treatment of nasopharyngeal carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Tanshinone II A enhances pyroptosis and represses cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway

2020

View full text Add to dashboard Cite

Cervical cancer is the fourth most common cancer in women globally. Lack of effective pharmacotherapies for cervical cancer mainly attributed to an elusive understanding of the mechanism underlying its pathogenesis. Pyroptosis plays a key role in inflammation and cancer. Our study identified microRNA (miR) 145 (miR-145)/gasdermin D (GSDMD) signaling pathway as critical mediators in the effect of tanshinone II A on HeLa cells. In the present study, we found that treatment of tanshinone II A led to an obvious repression of cell proliferation and an increase in apoptosis on HeLa cells, especially in high concentration. Compared with the controlled group, tanshinone II A enhanced the activity of caspase3 and caspase9. Notably, the results demonstrated that tanshinone II A regulated cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway. Treatment of tanshinone II A significantly up-regulated the expression of GSDMD and miR-145. After transfection of si-miR-145 plasmids, the effects of tanshinone II A on HeLa cells were converted, including cell proliferation, apoptosis and pyroptosis. In addition, the results showed that tanshinone II A treatment altered the expression level of PI3K, p-Akt, NF-kB p65 and Lc3-I. Collectively, our findings demonstrate that tanshinone II A exerts anticancer activity on HeLa cells by regulating miR-145/GSDMD signaling. The present study is the first time to identify miR-145 as a candidate target in cervical cancer and show an association between miR-145 and pyroptosis, which provides a novel therapy for the treatment of cervical cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Tanshinone II A enhances pyroptosis and represses cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway

2020

View full text Add to dashboard Cite

show abstract

“…Systematic search; duplicate removal; and screening of titles, abstracts, and full text yielded eight eligible articles [ 41 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. The expression of miR-145 was significantly reduced in thyroid cancer compared with normal cells [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

A miRNA-Based Prognostic Model to Trace Thyroid Cancer Recurrence

Toraih

Fawzy

Niu

et al. 2022

Cancers

View full text Add to dashboard Cite

Papillary thyroid carcinomas (PTCs) account for most endocrine tumors; however, screening and diagnosing the recurrence of PTC remains a clinical challenge. Using microRNA sequencing (miR-seq) to explore miRNA expression profiles in PTC tissues and adjacent normal tissues, we aimed to determine which miRNAs may be associated with PTC recurrence and metastasis. Public databases such as TCGA and GEO were utilized for data sourcing and external validation, respectively, and miR-seq results were validated using quantitative real-time PCR (qRT-PCR). We found miR-145 to be significantly downregulated in tumor tissues and blood. Deregulation was significantly related to clinicopathological features of PTC patients including tumor size, lymph node metastasis, TNM stage, and recurrence. In silico data analysis showed that miR-145 can negatively regulate multiple genes in the TC signaling pathway and was associated with cell apoptosis, proliferation, stem cell differentiation, angiogenesis, and metastasis. Taken together, the current study suggests that miR-145 may be a biomarker for PTC recurrence. Further mechanistic studies are required to uncover its cellular roles in this regard.

show abstract

“…In literature, several papers reported that miR-145 is downregulated in thyroid cancer where it acts as a tumor suppressor by impairing cancer cell growth, cell motility, and metastasis through targeting AKT3 [ 59 ] and RAB5C [ 60 ] genes or by regulating the NF- κ B pathway [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines

Orlandella¹,

Mirabelli²,

Stefano

et al. 2022

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Among natural macromolecules, the polyphenol extract from Annurca flesh (AFPE) apple could play a potential therapeutic role for a large spectrum of human cancer also by exerting antioxidant properties. Thyroid cancer is a common neoplasia in women, and it is in general responsive to treatments although patients may relapse and metastasize or therapy-related side effects could occur. In this study, we explored the effects of AFPE on papillary (TPC-1) and anaplastic (CAL62) thyroid cancer cell line proliferation and viability. We found that AFPE exposure induced a reduction of cell proliferation and cell viability in dose-dependent manner. The effect was associated with the reduction of phosphorylation of Rb protein. To study the mechanisms underlying the biological effects of AFPE treatment in thyroid cancer cells, we investigated the modulation of miRNA (miR) expression. We found that AFPE treatment increased the expression of the miR-141, miR-145, miR-200a-5p, miR-425, and miR-551b-5p. Additionally, since natural polyphenols could exert their beneficial effects through the antioxidant properties, we investigated this aspect, and we found that AFPE treatment reduced the production of reactive oxygen species (ROS) in CAL62 cells. Moreover, AFPE pretreatment protects against hydrogen peroxide-induced oxidative stress in thyroid cancer cell lines. Taken together, our findings suggest that AFPE, by acting at micromolar concentration in thyroid cancer cell lines, may be considered a promising adjuvant natural agent for thyroid cancer treatment approach.

show abstract

MiR‐145 inhibits the migration and invasion of papillary thyroid carcinoma cells through NF‐κB pathway regulation

Cited by 13 publications

References 26 publications

Tanshinone II A enhances pyroptosis and represses cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway

Tanshinone II A enhances pyroptosis and represses cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway

A miRNA-Based Prognostic Model to Trace Thyroid Cancer Recurrence

Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines

Contact Info

Product

Resources

About