miR‐144 downregulation increases bladder cancer cell proliferation by targeting <scp>EZH</scp>2 and regulating <scp>W</scp>nt signaling

Guo, Yansu; Li, Ying; Tian, Ye; Yang, Pei; Zhu, Yichen; Wang, Zhipeng; Qiu, Feng; Lin, Jun

doi:10.1111/febs.12417

Cited by 132 publications

(114 citation statements)

References 30 publications

(43 reference statements)

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“…Based upon the abundant data accumulated during the past two decades, scientists began to argue for use of miRNAs as novel therapeutic targets for various malignancies (33). MiR-144 has been implicated in both oncogenic and tumorsuppressive roles in different tumors, but its role in DLBCL is not yet clarified (34)(35)(36). Although research on the true biologic relevance of miR-144 in cancer is still in its infancy, in this study we focused on the expression and function of miR-144 in DLBCL.…”

Section: Discussionmentioning

confidence: 99%

A Critical Role of miR-144 in Diffuse Large B-cell Lymphoma Proliferation and Invasion

Wang

et al. 2016

Cancer Immunology Research

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MicroRNAs are endogenous noncoding RNAs that play important roles in a wide variety of biologic processes such as apoptosis, development, aging, and tumorigenesis. The B-cell lymphoma 6 (BCL6) transcriptional repressor has emerged as a critical therapeutic target in diffuse large B-cell lymphomas (DLBCL), but the mechanisms regulating BCL6 are still unclear. In the current study, we screened the microRNA expression profiles in DLBCL specimens and cell lines by qRT-PCR and found that the expression of miR-144 was significantly downregulated in DLBCL tissues and cell lines and negatively correlated with BCL6 expression. We further demonstrated that BCL6 was the direct target gene of miR-144, and miR-144 suppressed the expression of BCL6 via binding the 3 0 untranslated region of BCL6 mRNA. Biologically, forced expression of miR-144 significantly attenuated cell proliferation and invasion of OCI-Ly3 cells in vitro, and the tumorsuppressor effect of miR-144 was also confirmed using a xenograft mouse model in vivo. Taken together, our results reveal that miR-144 regulates BCL6 in DLBCL and provide a rationale for developing strategies that target miR-144 as a therapeutic intervention for DLBCL.

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Section: Discussionmentioning

confidence: 99%

A Critical Role of miR-144 in Diffuse Large B-cell Lymphoma Proliferation and Invasion

Wang

et al. 2016

Cancer Immunology Research

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“…MiR-144 has recently been shown to be downregulated in bladder cancer, which leads to loss of inhibition of zeste homolog 2 and subsequent activation of Wnt/β-catenin signaling with ensuing cellular proliferation. 45 Xie et al 46 showed that miR-497 is underexpressed in hepatocellular carcinoma and that this miRNA is a negative regulator of checkpoint kinase 1, which functions in cell cycle arrest after DNA damage. The roles of these tumor suppressor miRNAs have not yet been explored in the biology of adipocytic neoplasms.…”

Section: Discussionmentioning

confidence: 99%

A selective screening platform reveals unique global expression patterns of microRNAs in a cohort of human soft-tissue sarcomas

Balkhi

Ladner

et al. 2016

Laboratory Investigation

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Sarcomas are malignant heterogenous tumors of mesenchymal derivation. Emerging data suggest that miRNA might have a causal role in sarcomagenesis. Herein, we used a selective miRNA screening platform to study the comparative global miRNA expression signatures in a cohort of human sarcomas with the caveat that comparisons between tumor and non-tumor cells were performed from the same patients using formalin-fixed paraffin-embedded tissue. Five histologic types were examined that included: myxoid liposarcoma, well-differentiated liposarcoma, dedifferentiated liposarcoma, pleomorphic rhabdomyosarcoma, and synovial sarcoma. In addition, soft-tissue lipomas and normal fat were included as a separate set of controls for the lipogenic tumors. Clustering analysis showed a distinct global difference in expression patterns between the normal and sarcoma tissues. Expression signatures in an unsupervised hierarchical clustering analysis revealed tight clustering in synovial and myxoid liposarcomas, and the least clustering was observed in the pleomorphic rhabdomyosarcoma subtype. MiR-145 showed underexpression in pleomorphic rhabdomyosarcoma, well-differentiated liposarcoma, and synovial sarcoma. Unexpectedly, we found that a set of muscle-specific microRNAs (miRNAs; myomiRs): miR-133, miR-1, and miR-206 was significantly underexpressed in well-differentiated liposarcoma and synovial sarcoma, suggesting that they may function as tumor suppressors as described in muscle-relevant rhabdomyosarcomas. In addition, a tight linear progression of miRNA expression was identified from normal fat to dedifferentiated liposarcoma. These results suggest that miRNA expression profiles could elucidate classes of miRNAs that may elicit tumor-relevant activities in specific sarcoma subtypes. Sarcomas are rare malignant heterogeneous tumors of mesenchymal derivation with over 50 histologic types described. 1 The pathogenesis and biology of many of the different histologic subtypes remain poorly understood. Recent progress in understanding the biology of sarcomas has identified distinct molecular and pathologic entities within these heterogeneous groups of tumors that have paved the way for development of targeted therapies. There are emerging data that seem to suggest that miRNA might be a driver in sarcomagenesis with potential therapeutic implications. microRNAs (miRNAs) are non-coding strands of RNA of 22 nucleotides in length that regulate the expression of genes involved in processes such as development, differentiation, cell proliferation, metabolism, cell death, viral infection, and cancer. 2,3 They have a broad effect through base pairing with mRNA at their 3′ untranslated region to inhibit translation or to target the mRNA for degradation. Bioinformatics predictions have shown that miRNAs regulatẽ 30% of mammalian protein-coding genes. Their role in the molecular biology of cancers is well-established partly

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“…miR-144 has been revealed to be involved in multiple types of human malignancy (4,5). In addition, miR-144 was reported to act as a tumor suppressor in certain types of cancer, including bladder (4), non-small cell lung (6) and colorectal (7) cancer.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-144 acts as a tumor suppressor by targeting Rho-associated coiled-coil containing protein kinase 1 in osteosarcoma cells

Huang

Shi

et al. 2015

Molecular Medicine Reports

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Abstract. MicroRNAs (miRs) have been demonstrated to be associated with multiple processes in the development and progression of human malignancies. Previous studies have observed aberrant downregulation of miR-144 in several types of cancer, including osteosarcoma. However, the function of miR-144 and the underlying mechanism in osteosarcoma remain to be elucidated. The present study indicated that miR-144 was markedly downregulated in osteosarcoma tissues and cell lines compared with that in the normal controls. Restoration of miR-144 significantly inhibited cell proliferation, migration and invasion of MG-63 osteosarcoma cells. In addition, Rho-associated coiled-coil containing protein kinase 1 (ROCK1) was identified as a novel target of miR-144 in MG-63 osteosarcoma cells. Furthermore, knockdown of ROCK1 suppressed the proliferation, migration and invasion of MG-63 osteosarcoma cells to a similar extent to the effects of miR-144 overexpression. In addition, the mRNA expression of ROCK1 was increased in osteosarcoma tissues and was negatively correlated with the expression of miR-144. In conclusion, the results of the present study suggested that miR-144 acts as a tumor suppressor by targeting ROCK1 in osteosarcoma.

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miR‐144 downregulation increases bladder cancer cell proliferation by targeting EZH2 and regulating Wnt signaling

Cited by 132 publications

References 30 publications

A Critical Role of miR-144 in Diffuse Large B-cell Lymphoma Proliferation and Invasion

A Critical Role of miR-144 in Diffuse Large B-cell Lymphoma Proliferation and Invasion

A selective screening platform reveals unique global expression patterns of microRNAs in a cohort of human soft-tissue sarcomas

MicroRNA-144 acts as a tumor suppressor by targeting Rho-associated coiled-coil containing protein kinase 1 in osteosarcoma cells

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