miR-143 promotes angiogenesis and osteoblast differentiation by targeting HDAC7

Abstract

The regulation of bone formation and detailed mechanisms are still largely elusive, and the roles of microRNAs in this process have attracted much attention. Recently, a specific subtype of CD31 hi endomucin hi (CD31 hi EMCN hi) endothelium has been identified to promote bone formation, together with osteoblast development. However, the role of microRNA143 in the generation of CD31 hi EMCN hi endothelium and bone formation remains unknown. In this study, we found that miR-143 was expressed both in osteoblast cells and CD31 hi EMCN hi endothelial cells. Serum miR-143 level was negatively correlated with age in humans. Overexpression of miR-143 promoted osteoblast formation and angiogenic effects. Furthermore, CD31 hi Emcn hi vessels and osteoblast formation were significantly inhibited in miR-143 knockout mice. Mechanistically, inhibitor HDAC7 was directly targeted by miR-143 and knockdown of HDAC7 was found to rescue the function of miR-143 deficiency. Thus, miR-143 promotes angiogenesis coupling with osteoblast differentiation by targeting HDAC7, which may serve as a potential target in angiogenic and osteogenic diseases.

Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1

“… 46 A number of miRNAs have been identified in the regulation of osteoblast differentiation. 47 , 48 , 49 These findings raise the possibility that osteogenic differentiation during bone remodeling can be regulated by miRNAs derived from bone cells. In our study, we identified that miR-324 was highly expressed in osteoclast-derived sEVs using miRNAs microarray analysis.…”

Diagnostic Performance of a Panel of miRNAs (OsteomiR) for Osteoporosis in a Cohort of Postmenopausal Women

“…Osterix seems to be the direct target of miR-143 in suppressing osteogenic differentiation of MC3T3-E1 cells [ 37 ]. In miR-143 knockout mice, osteoblast differentiation was significantly inhibited [ 38 ]. All these data fit our results of high serum levels of miR-143-3p indicating a high risk of osteoporosis (OR 2.81).…”

Extracorporeal Shock Wave Therapy Promotes Osteogenic Differentiation in a Rabbit Osteoporosis Model

“…The rabbits were housed in barrier housing conditions in the Animal Center of Fourth Military Medical University. Gengerate a rabbit osteoporosis model, we used the protocol as described previously ( 8 ). Briefly, we fasted the rabbits for 24 h prior to surgery.…”

“…Western blotting was performed as previously described ( 8 ). We used M-PER Protein Extraction Reagent (CST) supplemented with a protease inhibitor cocktail (CST) to lyse cells.…”