miR-143 inhibits proliferation and induces apoptosis of mammary epithelial cells in dairy goat

Ji, Zhibin; Wang, Guizhi; Hou, Lei; Liu, Zhaohua; Wang, Jianmin; Chao, Tianle

doi:10.1080/19768354.2016.1165288

Cited by 10 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ji et al (2015) showed that prolactin receptor is a target gene of miR-135a that may play a key role in regulating the development and lactation of caprine mammary gland [ 30 ]. MiR-143 is a crucial post-transcription regulator that is related to caprine mammary cell survival, and it may have an effect on the development, lactation or involution of mammary gland [ 31 ]. Dong et al (2013) suggested that miR-423-5p, miR-378 and miR-7 could have important regulatory functions during lactation with respect to milk ingredient transport and milk ingredient synthesis in the mammary gland [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

Hou

Song

et al. 2017

BMC Genet

View full text Add to dashboard Cite

BackgroundMicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods.ResultThis study identified 131 differentially expressed miRNAs (P < 0.05 and log2 colostrum normalized expression (NE)/peak lactation NE > 1 or log2 colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and log2 colostrum NE/peak lactation NE > 1 or log2 colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway.ConclusionsOur finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-017-0498-2) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

Hou

Song

et al. 2017

BMC Genet

View full text Add to dashboard Cite

show abstract

“…miR-143 appeared to be expressed at relatively high levels in adipose or mammary gland tissues ( Gu, Eleswarapu & Jiang, 2007 ). Our study showed that miR-143-induced mammary epithelial cell arrest in the G0/G1 phase delayed the progression of the cell cycle and inhibited cell proliferation ( Ji et al, 2016 ). Furthermore, miR-143 inhibits the proliferation and promotes the apoptosis of mammary gland epithelial cells by targeting and regulating the expression of Ndfip1 ( Ji et al, 2019 ).…”

Section: Discussionmentioning

confidence: 89%

Evaluation of key miRNAs during early pregnancy in Kazakh horse using RNA sequencing

Liu

Fang

Sun

et al. 2021

PeerJ

View full text Add to dashboard Cite

Background miRNA has an important role in cell differentiation, biological development, and physiology. Milk production is an important quantitative trait in livestock and miRNA plays a role in the amount of milk produced. Methods The role of regulatory miRNAs involved in equine milk production is not fully understood. We constructed two miRNA libraries for Kazakh horse milk production from higher-producing (H group) and lower-producing (L group) individuals, and used RNA-Seq technology to identify the differentially expressed miRNAs between the two milk phenotypes of Kazakh horses. Results A total of 341 known and 333 novel miRNAs were detected from the H and L groups, respectively. Eighty-three differentially expressed miRNAs were identified between the H and L group s, of which 32 were known miRNAs (27 were up-regulated, five were down-regulated) and 51 were novel miRNAs (nine were up-regulated, 42 were down-regulated). A total of 2,415 genes were identified. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that these genes were annotated to mammary gland development, mammary gland morphogenesis, tissue development and PI3K-Akt signaling pathways, insulin signaling pathway and TGF-beta signaling pathway, among others. Five miRNAs (miR-199a-3p, miR143, miR145, miR221, miR486-5p) were identified as affecting horse milk production and these five miRNAs were validated using qRT-PCR. Conclusions We described a methodology for the transcriptome-wide profiling of miRNAs in milk, which may help the design of new intervention strategies to improve the milk yield of Kazakh horses.

show abstract

“…An increasing amount of experimental evidence demonstrated that the proliferation and apoptosis of mammary epithelial cells had influences on the development of the mammary gland, milk secretion, and lactation [ 25 , 26 , 27 ]. Using the siRNA strategy, we found that both AGPAT1 and AGPAT6 knockdown could suppress proliferation and apoptosis of BuMECs.…”

Section: Discussionmentioning

confidence: 99%

Novel Insight into the Potential Role of Acylglycerophosphate Acyltransferases Family Members on Triacylglycerols Synthesis in Buffalo

Duan

et al. 2022

IJMS

View full text Add to dashboard Cite

Acylglycerophosphate acyltransferases (AGPATs) are the rate-limiting enzymes for the de novo pathway of triacylglycerols (TAG) synthesis. Although AGPATs have been extensively explored by evolution, expression and functional studies, little is known on functional characterization of how many members of the AGPAT family are involved in TAG synthesis and their impact on the cell proliferation and apoptosis. Here, 13 AGPAT genes in buffalo were identified, of which 12 AGPAT gene pairs were orthologous between buffalo and cattle. Comparative transcriptomic analysis and real-time quantitative reverse transcription PCR (qRT-PCR) further showed that both AGPAT1 and AGPAT6 were highly expressed in milk samples of buffalo and cattle during lactation. Knockdown of AGPAT1 or AGPAT6 significantly decreased the TAG content of buffalo mammary epithelial cells (BuMECs) and bovine mammary epithelial cells (BoMECs) by regulating lipogenic gene expression (p < 0.05). Knockdown of AGPAT1 or AGPAT6 inhibited proliferation and apoptosis of BuMECs through the expression of marker genes associated with the proliferation and apoptosis (p < 0.05). Our data confirmed that both AGPAT1 and AGPAT6 could regulate TAG synthesis and growth of mammary epithelial cells in buffalo. These findings will have important implications for understanding the role of the AGPAT gene in buffalo milk performance.

show abstract

miR-143 inhibits proliferation and induces apoptosis of mammary epithelial cells in dairy goat

Cited by 10 publications

References 28 publications

Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

Evaluation of key miRNAs during early pregnancy in Kazakh horse using RNA sequencing

Novel Insight into the Potential Role of Acylglycerophosphate Acyltransferases Family Members on Triacylglycerols Synthesis in Buffalo

Contact Info

Product

Resources

About