MiR-143 and rat embryo implantation

Shen, Tian; Su, Xing; Qi, Lu; Jin, Xiaohua; Hu, Yi; Ma, Xu; Xia, Hong-Fei

doi:10.1016/j.bbagen.2014.11.023

Cited by 15 publications

(9 citation statements)

References 46 publications

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“…Within our short list of 39 conditioned media exclusive extracellular miRNAs (Supplementary Table 1), we noticed the spermatozoan miR-34c (Liu et al, 2012) was detected within the array results and raises the consideration that, unlike the human pre-implantation secretome studies that utilize ICSI inseminated zygotes, unsuccessful zona-bound spermatozoa may need to be considered as potential miRNA contributors to any naturally fertilized blastocyst secretome dataset (Liu et al, 2012); the zona pellucidae can remain decorated with spermatozoa for days after fertilization (Xia et al, 2001), any of which may become dislodged during culture. It is interesting that differential expression of many of these miRNAs are reported within murine uterine studies, including association with decidualization (Tian et al, 2015) and spatial representation across implantation sites (Li et al, 2015). The sequences in Supplementary Table 1 not associated with the miR-290 polycistron and not interrogated in this study, in addition to lesser filtered sequences found within our publicly available array data (GEO accession GSE138285 1 ), may be of relevance to the embryo-uterine dialog at the time of implantation, and certainly all merit further investigation.…”

Section: Late-stage Murine Blastocysts Release Mature Mirna Sequences Into Culture Mediamentioning

confidence: 99%

Murine Blastocysts Release Mature MicroRNAs Into Culture Media That Reflect Developmental Status

et al. 2021

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Extracellular microRNA (miRNA) sequences derived from the pre-implantation embryo have attracted interest for their possible contributions to the ongoing embryonic–uterine milieu, as well as their potential for use as accessible biomarkers indicative of embryonic health. Spent culture media microdroplets used to culture late-stage E4.0 murine blastocysts were screened for 641 mature miRNA sequences using a reverse transcription–quantitative polymerase chain reaction–based array. We report here 39 miRNAs exclusively detected in the conditioned media, including the implantation-relevant miR-126a-3p, miR-101a, miR-143, and miR-320, in addition to members of the highly expressed embryonic miR-125 and miR-290 families. Based on these results, an miRNA panel was assembled comprising five members of the miR-290 family (miR-291-295) and five conserved sequences with significance to the embryonic secretome (miR-20a, miR-30c, miR-142-3p, miR-191, and miR-320). Panel profiling of developing embryo cohort lysates and accompanying conditioned media microdroplets revealed extensive similarities in relative quantities of miRNAs and, as a biomarker proof of concept, enabled distinction between media conditioned with differently staged embryos (zygote, 4-cell, and blastocyst). When used to assess media conditioned with embryos of varying degrees of degeneration, the panel revealed increases in all extracellular panel sequences, suggesting cell death is an influential and identifiable factor detectable by this assessment. In situ hybridization of three panel sequences (miR-30c, miR-294, and miR-295) in late-stage blastocysts revealed primarily inner cell mass expression with a significant presence of miR-294 throughout the blastocyst cavity. Furthermore, extracellular miR-290 sequences responded significantly to high centrifugal force, suggesting a substantial fraction of these sequences may exist within a vesicle such as an exosome, microvesicle, or apoptotic bleb. Together, these results support the use of extracellular miRNA to assess embryonic health and enable development of a non-invasive viability diagnostic tool for clinical use.

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Section: Late-stage Murine Blastocysts Release Mature Mirna Sequences Into Culture Mediamentioning

confidence: 99%

Murine Blastocysts Release Mature MicroRNAs Into Culture Media That Reflect Developmental Status

et al. 2021

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“…These results are indicating that miRNAs may be involved in the maintenance and establishment of embryonic survival and embryonic implantation during mammalian embryo development. Moreover, individual functional assay revealed endometrial miR-143, which was upregulated during implantation phase in rat endometrium due to blastocysts activation and uterine decidualization, is believed to be involved embryo implantation by promoting cell proliferation and invasion [138]. Interestingly, miRNA of endometrial origin not only regulate embryo implantation by regulation of endometrial cells, but also could be taken by the embryo to activate embryonic genes.…”

Section: The Role Of Maternal Mirnas In Embryo Implantationmentioning

confidence: 99%

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Salilew‐Wondim

Gebremedhn

Hoelker

et al. 2020

IJMS

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The genetic codes inscribed during two key developmental processes, namely gametogenesis and embryogenesis, are believed to determine subsequent development and survival of adult life. Once the embryo is formed, its further development mainly depends on its intrinsic characteristics, maternal environment (the endometrial receptivity), and the embryo–maternal interactions established during each phase of development. These developmental processes are under strict genetic regulation that could be manifested temporally and spatially depending on the physiological and developmental status of the cell. MicroRNAs (miRNAs), one of the small non-coding classes of RNAs, approximately 19–22 nucleotides in length, are one of the candidates for post-transcriptional developmental regulators. These tiny non-coding RNAs are expressed in ovarian tissue, granulosa cells, testis, oocytes, follicular fluid, and embryos and are implicated in diverse biological processes such as cell-to-cell communication. Moreover, accumulated evidences have also highlighted that miRNAs can be released into the extracellular environment through different mechanisms facilitating intercellular communication. Therefore, understanding miRNAs mediated regulatory mechanisms during gametogenesis and embryogenesis provides further insights about the molecular mechanisms underlying oocyte/sperm formation, early embryo development, and implantation. Thus, this review highlights the role of miRNAs in mammalian gametogenesis and embryogenesis and summarizes recent findings about miRNA-mediated post-transcriptional regulatory mechanisms occurring during early mammalian development.

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“…In recent years, accumulated studies have suggested that microRNAs and their target genes play essential roles in embryo implantation and development 18 . Examples include miR-451/Ankrd46 19 , miR-429/Pcdh820 20 , miR-181/LIF 21 , miR-145/IGF1R 22 , miR-143/Lifr 23 , and let-7/mucin1 24 . MiR-125b is the human orthologue of lin-4, which is essential for embryonic proliferation and differentiation 25 .…”

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confidence: 99%

MiR-125b regulates endometrial receptivity by targeting MMP26 in women undergoing IVF-ET with elevated progesterone on HCG priming day

Chen

Zhao

et al. 2016

Sci Rep

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On the women undergoing IVF-ET with elevated progesterone on human chorionic gonadotrophin priming, the assisted reproductive technology outcome is poor. But, due to the unknown mechanism of this process, no effective method has been found to overcome this difficulty. Here, we investigated the roles of miR-125b and its target gene, MMP26, in endometrial receptivity (ER) in these women. The expression of miR-125b was significantly up-regulated in EECs in women with elevated progesterone during the window of implantation, and it showed a progesterone-dependent effect in vitro. Similarly, the expression of miR-125b was significantly up-regulated in the preimplantation period, and was down-regulated in the implantation period and the post-implantation period in mouse EECs. In addition, miR-125b showed a greater decrease at implantation sites than it did at interimplantation sites. The luciferase report assay demonstrated that MMP26 is a target gene of miR-125b. And the expression profile of MMP26 showed an inverse relationship with miR-125b in vivo and in vitro. Overexpression of miR-125b in human EECs inhibited cell migration and invasion. Gain-of-function of miR-125b induced a significant decrease in the number of implantation sites. In conclusion, these data shed new light on how miR-125b triggers ER decline through the regulation of MMP26 function.

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MiR-143 and rat embryo implantation

Cited by 15 publications

References 46 publications

Murine Blastocysts Release Mature MicroRNAs Into Culture Media That Reflect Developmental Status

Murine Blastocysts Release Mature MicroRNAs Into Culture Media That Reflect Developmental Status

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

MiR-125b regulates endometrial receptivity by targeting MMP26 in women undergoing IVF-ET with elevated progesterone on HCG priming day

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