miR‑142‑5p promotes renal cell tumorigenesis by targeting TFAP2B

Abstract

The transcription factor AP-2 β ( TFAP2B ) serves an important role in kidney development. MicroRNAs (miRNAs) regulate carcinogenic pathways and have gained increasing attention owing to their association with human clear cell renal cell carcinoma (ccRCC) tumorigenesis. However, whether miRNAs could affect renal cell tumorigenesis by regulating TFAP2B expression has not been identified. The aim of this study was to investigate the effects of miRNA on TFAP2B and its potential role in cell growth, invasion and migration. PCR, western blot and dual luciferase reporter assays were performed to analyze the effects of miR-142-5p on TFAP2B . Furthermore, MTT, flow cytometry, wound healing and Transwell migration assays were used to analyze the effect of miR-142-5p on cell proliferation and migration. The results demonstrated that miR-142-5p targeted TFAP2B and downregulated the expression of TFAP2B at the mRNA and protein levels, promoting cell proliferation and migration in two ccRCC cell lines, 786-O and A-498. This phenomenon supported the theory that miR-142-5p may function as an oncogene in ccRCC. The potential clinical significance of miR-142-5p as a biomarker and a therapeutic target provides rationale for further investigation into miR-142-5p-mediated molecular pathways and how these may be associated with ccRCC development.

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

“…Other miRNAs targeted genes involved in cell–cell interaction, cell death, cell survival and cellular assembly and organization network including miR-142-5p, miR-151-3p, miR-218-1-3p, miR-335, and miR-340 related with AGO2, AGO3, CDH1, GABRA3 ( Figure 3 C). These results are similar to previous reports in renal cell carcinoma [ 34 ] and thyroid cancer [ 35 ]. The results of network analysis are presented in Figure 3 .…”

Gut Microbially Produced Indole-3-Propionic Acid Inhibits Atherosclerosis by Promoting Reverse Cholesterol Transport and Its Deficiency Is Causally Related to Atherosclerotic Cardiovascular Disease

“…42 Over the years, there have been a growing body of studies that underscores the role of miR-142-5p in pathophysiological development of many diseases, such as epilepticus, 43 diabetic retinopathy, 44 nonalcoholic fatty liver disease, 45 and cancers. 46 Studies concerning the role of miR-142-5p in cardiovascular diseases is still lacking, but there is some evidence. Xu et al 47 found that miR-142-5p level is significantly increased in the atherosclerotic plaque obtained from ApoE −/− mice.…”

Generation of tamoxifen‐inducible Tfap2b‐CreERT2 mice using CRISPR‐Cas9

“…Numerous studies have highlighted the essential roles of TFAP2B, not only in the various tissues affected by the Char syndrome, but also in the neural processes, tumorigenesis, and craniofacial development (Fu et al, 2014; Fu et al, 2019; Hu & Bringmann, 2023; Ikram et al, 2016; Satoda et al, 2000; Zhu et al, 2020). Our lineage tracing has revealed that Tfap2b+ cells are progenitors for tooth development.…”