MiR‐142‐3p represses TGF‐β‐induced growth inhibition through repression of TGFβR1 in non‐small cell lung cancer

Li, Zhe; Xu, Gelin; Wang, Longqiang; Yang, Haiping; Liu, Xia; Zhao, Jun; Zhang, Hong Tao

doi:10.1096/fj.13-247288

Cited by 88 publications

(132 citation statements)

References 33 publications

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“…miRNAs target numerous genes, including HIF, mTOR, VEGF and VHL, through translational inhibition or the induction of mRNA degradation, which suggests that miRNAs function as important factors in RCC initiation and development (36,37). Previous studies have reported that miR-142-3p is dysregulated in various types of cancer (22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33), and may therefore function as a biomarker. The expression of miR-142-3p in RCC has not yet been validated by qPCR.…”

Section: Discussionmentioning

confidence: 99%

“…Downregulation of miR-142-3p in thyroid neoplastic tissues contributes to thyroid follicular tumorigenesis through the targeting of ASH1L and MLL1, and subsequently promotes its tumor suppressive function (23). In non-small cell lung cancer, overexpression of miR-142-3p represses transforming growth factor (TGF)-induced growth inhibition through the repression of TGFβ receptor 1 (26). However, a previous study investigating miR-142-3p in human acute lymphoblastic leukemia demonstrated that miR-142-3p inhibits cell proliferation by targeting the MLL-AF4 oncogene (27).…”

Section: Discussionmentioning

confidence: 99%

“…Previous microarray chip studies have demonstrated that miR-142-3p is overexpressed in RCC tissues compared with adjacent normal or benign kidney tissues (18)(19)(20)(21). It has also been reported that miR-142-3p is dysregulated in malignancies of the breast (22), thyroid (23), liver (24), stomach (25), lung (26), blood (27,28), colorectum (29), testes (30), esophagus (31), head and neck (32), and bone (33). The present study establishes the oncogenic role of miR-142-3p in RCC, demonstrating how it regulates cell migration, proliferation and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

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Oncogenic microRNA-142-3p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Chen

Jin

et al. 2015

Oncology Letters

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Abstract. MicroRNAs (miRNAs/miRs) serve an important role in the regulation of carcinogenic pathways. RCC is the most prevalent kidney cancer that occurs in adults. miRNAs have gained increasing attention due to their association with RCC tumorigenesis, serving as biomarkers for early detection and progression monitoring, and as potential targets for molecular therapy. Upregulation of miRNA-142-3p has been previously identified in RCC tissues by microarray profile, however, its expression and function in RCC have not yet been validated. In the present study, quantitative polymerase chain reaction was performed to quantify the relative expression of miR-142-3p in 53 paired RCC and adjacent normal tissues. Furthermore, wound healing, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry assays were performed to analyze the impacts of miR-142-3p on cellular migration, proliferation and apoptosis. The results demonstrated that miR-142-3p was significantly upregulated in RCC tissues compared with adjacent normal tissues. Downregulation of miR-142-3p, induced by chemically synthesized miR-142-3p inhibitor, significantly suppressed cell migration and proliferation, and promoted cell apoptosis in 786-O and ACHN cells, supporting the theory that miR-142-3p may function as an oncogene in RCC. The potential clinical significance of miR-142-3p, as a biomarker and therapeutic target, provides rationale for further investigation into the miR-142-3p-mediated molecular pathway and how it is associated with RCC development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oncogenic microRNA-142-3p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Chen

Jin

et al. 2015

Oncology Letters

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“…In addition, ESCC patients with high expression levels of miR-142 exhibited poorer survival rates compared with those with low expression levels of miR-142 (16), whereas upregulated levels of miR-142 were involved in aggressive non-small-cell lung carcinoma (NSCLC) (21,22). However, a recent study has suggested that miR-142 may be associated with the suppression of NSCLC cells (23). Wang et al (24) reported that miR-142 was upregulated by the NGX6 metastasis suppressor gene in colon cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Demethylation of microRNA-142 induced by demethylation agents plays a suppressive role in osteosarcoma cells

Ding¹,

Hu²,

Ni³

et al. 2015

Oncology Letters

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Abstract. Osteosarcoma (OS), the most common malignant bone tumor, occurs mainly in adolescents and young adults, with a morbidity of ~5 cases per million. The expression levels of microRNAs (miRNAs) as tumor suppressors were recently found to be downregulated in OS. Certain alterations of miRNAs and the possible mechanisms through which miRNAs affect cell proliferation and migration in OS were recently found to be correlated with methylation epigenetic mechanisms. In this study, it was demonstrated that, due to hypermethylation, the expression level of miRNA-142 (miR-142) was significantly downregulated in OS tissues and cells compared with that in control samples. The present study demonstrated an increased expression of miR-142 in Saos-2 and MG63 cells treated with demethylation agents, suggesting that the effect of such agents on cell growth, inhibition of invasion and cell cycle retardation may be mediated by miR-142 in OS.

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“…The viral miRNAs maintained the same seed sequence as the human cellular miRNA 142-3p (Fig. 10C), which is known to antagonize growth factor signaling influencing cellular proliferation (58).…”

Section: Figmentioning

confidence: 99%

Complete Genome Sequence of Pig-Tailed Macaque Rhadinovirus 2 and Its Evolutionary Relationship with Rhesus Macaque Rhadinovirus and Human Herpesvirus 8/Kaposi's Sarcoma-Associated Herpesvirus

Bruce

Thouless

Haines

et al. 2015

J Virol

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Two rhadinovirus lineages have been identified in Old World primates. The rhadinovirus 1 (RV1) lineage consists of human herpesvirus 8, Kaposi's sarcoma-associated herpesvirus (KSHV), and closely related rhadinoviruses of chimpanzees, gorillas, macaques and other Old World primates. The RV2 rhadinovirus lineage is distinct and consists of closely related viruses from the same Old World primate species. Rhesus macaque rhadinovirus (RRV) is the RV2 prototype, and two RRV isolates, 26-95 and 17577, were sequenced. We determined that the pig-tailed macaque RV2 rhadinovirus, MneRV2, is highly associated with lymphomas in macaques with simian AIDS. To further study the role of rhadinoviruses in the development of lymphoma, we sequenced the complete genome of MneRV2 and identified 87 protein coding genes and 17 candidate microRNAs (miRNAs). A strong genome colinearity and sequence homology were observed between MneRV2 and RRV26-95, although the open reading frame (ORF) encoding the KSHV ORFK15 homolog was disrupted in RRV26-95. Comparison with MneRV2 revealed several genomic anomalies in RRV17577 that were not present in other rhadinovirus genomes, including an N-terminal duplication in ORF4 and a recombinative exchange of more distantly related homologs of the ORF22/ORF47 interacting glycoprotein genes. The comparison with MneRV2 has revealed novel genes and important conservation of protein coding domains and transcription initiation, termination, and splicing signals, which have added to our knowledge of RV2 rhadinovirus genetics. Further comparisons with KSHV and other RV1 rhadinoviruses will provide important avenues for dissecting the biology, evolution, and pathology of these closely related tumor-inducing viruses in humans and other Old World primates. IMPORTANCEThis work provides the sequence characterization of MneRV2, the pig-tailed macaque homolog of rhesus rhadinovirus (RRV). MneRV2 and RRV belong to the rhadinovirus 2 (RV2) rhadinovirus lineage of Old World primates and are distinct but related to Kaposi's sarcoma-associated herpesvirus (KSHV), the etiologic agent of Kaposi's sarcoma. Pig-tailed macaques provide important models of human disease, and our previous studies have indicated that MneRV2 plays a causal role in AIDS-related lymphomas in macaques. Delineation of the MneRV2 sequence has allowed a detailed characterization of the genome structure, and evolutionary comparisons with RRV and KSHV have identified conserved promoters, splice junctions, and novel genes. This comparison provides insight into RV2 rhadinovirus biology and sets the groundwork for more intensive next-generation (NextGen) transcript and genetic analysis of this class of tumor-inducing herpesvirus. This study supports the use of MneRV2 in pigtailed macaques as an important model for studying rhadinovirus biology, transmission and pathology. K aposi's sarcoma-associated herpesvirus/human herpesvirus 8 (KSHV) was first detected in 1994 in Kaposi's sarcoma (KS) lesions of individuals with human immunodeficiency virus (HIV)-...

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MiR‐142‐3p represses TGF‐β‐induced growth inhibition through repression of TGFβR1 in non‐small cell lung cancer

Cited by 88 publications

References 33 publications

Oncogenic microRNA-142-3p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Oncogenic microRNA-142-3p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Demethylation of microRNA-142 induced by demethylation agents plays a suppressive role in osteosarcoma cells

Complete Genome Sequence of Pig-Tailed Macaque Rhadinovirus 2 and Its Evolutionary Relationship with Rhesus Macaque Rhadinovirus and Human Herpesvirus 8/Kaposi's Sarcoma-Associated Herpesvirus

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