MiR-139 inhibits invasion and metastasis of colorectal cancer by targeting the type I insulin-like growth factor receptor

Shen, Ke; Liang, Qiannan; Xu, Ke; Cui, Daling; Jiang, Lin; Yin, Peihao; Lü, Yanhua; Li, Qi; Liu, Jianwen

doi:10.1016/j.bcp.2012.04.017

Cited by 134 publications

(120 citation statements)

References 37 publications

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“…This style of analyses has shown reliable results with a 0.25 FDR (Subramanian et al 2005), and for strong biological signals, even a false-positive rate of 50% can yield accurate results (Cloonan et al 2008). The accuracy of the analyses in this study is highlighted by our recapitulation of known biological functions for miR-139-5p (Wong et al 2011;Fan et al 2012;Shen et al 2012). miRNAs achieve specific regulation of cellular processes by concomitant suppression of a network of genes underlying the same function and/or pathways (Cloonan et al 2008, 2011 ;Shirdel et al 2011;Gennarino et al 2012).…”

Section: Discussionsupporting

confidence: 56%

miR-139-5p is a regulator of metastatic pathways in breast cancer

Krishnan

Steptoe

Martin

et al. 2013

RNA

135

115

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Metastasis is a complex, multistep process involved in the progression of cancer from a localized primary tissue to distant sites, often characteristic of the more aggressive forms of this disease. Despite being studied in great detail in recent years, the mechanisms that govern this process remain poorly understood. In this study, we identify a novel role for miR-139-5p in the inhibition of breast cancer progression. We highlight its clinical relevance by reviewing miR-139-5p expression across a wide variety of breast cancer subtypes using in-house generated and online data sets to show that it is most frequently lost in invasive tumors. A biotin pull-down approach was then used to identify the mRNA targets of miR-139-5p in the breast cancer cell line MCF7. Functional enrichment analysis of the pulled-down targets showed significant enrichment of genes in pathways previously implicated in breast cancer metastasis (P < 0.05). Further bioinformatic analysis revealed a predicted disruption to the TGFβ, Wnt, Rho, and MAPK/PI3K signaling cascades, implying a potential role for miR-139-5p in regulating the ability of cells to invade and migrate. To corroborate this finding, using the MDA-MB-231 breast cancer cell line, we show that overexpression of miR-139-5p results in suppression of these cellular phenotypes. Furthermore, we validate the interaction between miR-139-5p and predicted targets involved in these pathways. Collectively, these results suggest a significant functional role for miR-139-5p in breast cancer cell motility and invasion and its potential to be used as a prognostic marker for the aggressive forms of breast cancer.

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Section: Discussionsupporting

confidence: 56%

miR-139-5p is a regulator of metastatic pathways in breast cancer

Krishnan

Steptoe

Martin

et al. 2013

RNA

135

115

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“…Furthermore, Zhang et al (28) revealed that colorectal cancer cell viability, colony formation, nude mice tumor growth, cell migration and invasion are repressed by miR-139, and Luo et al (29) demonstrated that proliferation, invasion and metastasis of laryngeal squamous carcinoma cells are also reduced. miR-139 functions as a tumor suppressor in esophageal squamous cell carcinoma by cell proliferation, migration and invasion inhibition, apoptosis induction and G0/G1 phase cell cycle arrest (22). These results demonstrated that miR-139 is a promising target for cancer therapy.…”

Section: Discussionmentioning

confidence: 74%

“…Wong et al (21) reported that low miR-139 expression in hepatocellular carcinoma was significantly associated with poor patient prognosis and metastatic tumor characteristics, including venous invasion, microsatellite formation, an absence of tumor encapsulation and reduced differentiation. Shen et al (22) demonstrated that miR-139 expression was also downregulated in colorectal cancer tissues compared with that in adjacent non-tumorous tissues, and decreased miR-139 expression has also been associated with colorectal cancer progression and metastasis. Furthermore, in a study by Liu et al (23), miR-139 expression was demonstrated to be reduced in esophageal squamous cell carcinoma tissues, and this reduction was associated with lymph node metastasis.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑139 inhibits the proliferation, migration and invasion of gastric cancer cells by directly targeting ρ‑associated protein kinase 1

et al. 2018

Oncol Lett

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Abstract. The expression, function and underlying mechanisms of microRNA-139 (miR-139) in gastric cancer were investigated in the present study. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to detect miR-139 expression in gastric cancer tissues and cell lines. The effects of miR-139 overexpression on gastric cancer cell proliferation, migration and invasion were evaluated. ρ-associated protein kinase 1 (ROCK1) was predicted as a downstream target of miR-139 and its role in gastric cancer was assessed by bioinformatics analysis, luciferase reporter assay, RT-qPCR and western blot analysis. ROCK1 overexpression was established to investigate if the effects of miR-139 on gastric cancer cells may be attenuated. The results indicated that miR-139 was aberrantly downregulated in gastric cancer tissues and cell lines. Increased miR-139 expression reduced gastric cancer cell proliferation, migration and invasion. ROCK1 was demonstrated to be a direct target of miR-139 in gastric cancer and ROCK1 overexpression reversed the suppressive effects on gastric cancer cell proliferation, migration and invasion induced by miR-139 overexpression. The present study provides clear evidence demonstrating the anti-oncogenic activity of miR-139 in human gastric cancer, as mediated by the targeted downregulation of ROCK1.

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“…The current study reveals no association between the expression of miR-139-3p and lymph node status, perhaps because the LSCC samples were more heterogenous and the aim of abovementioned study was supraglottic LSCC. miR-139-3p was also found to be underexpressed in colorectal cancer [35]. Interestingly, Lin et al [19] note miR-139-3p up-regulation in CRC with liver metastasis, which may indicate that this miRNA plays a variable role as both oncogene and suppressor in different tumours.…”

Section: Discussionmentioning

confidence: 99%

New miRNA expression abnormalities in laryngeal squamous cell carcinoma

Cybula

Wieteska

Józefowicz-Korczyńska

et al. 2016

CBM

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Abstract. BACKGROUND:Although the development of novel diagnostic and treatment strategies concerning laryngeal cancer is highly intensive, the survival rate remains virtually unchanged. Small non-coding RNAs appear to be very promising biomarkers -and so remain the focus of extensive investigation in laryngeal cancer. OBJECTIVE: We examined the expression of five miRNA and five genes related to cancer whether they could be potential laryngeal cancer biomarkers. METHODS:We performed an analysis in 47 patients diagnosed with laryngeal cancer. The qPCR technique was used to investigate the expression profile. RESULTS: While miR-21-3p and miR-525-5p were found to be significantly up-regulated, miR-139-3p and miR-885-5p expression is lower in laryngeal cancer. Moreover, PIK3R1 and HACE1 were found to be also down-regulated. CONCLUSIONS: The change in miRNA expression is frequent than the expression of other tested genes. The expression of passenger strands such as miR-21-3p and miR-139-3p, which are rarely investigated, is also significantly affected in laryngeal cancer. While PIK3R1, HACE1, miR-139-3p, and miR-885-5p may act as tumor suppressor genes in the studied tumour type, miR-21-3p and miR-525-5p seem to have oncogenic properties. Our findings suggest that miR-885-5p and PIK3R1 are the best indicators for the classification of laryngeal cancer tissue and normal mucosa.

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MiR-139 inhibits invasion and metastasis of colorectal cancer by targeting the type I insulin-like growth factor receptor

Cited by 134 publications

References 37 publications

miR-139-5p is a regulator of metastatic pathways in breast cancer

miR-139-5p is a regulator of metastatic pathways in breast cancer

MicroRNA‑139 inhibits the proliferation, migration and invasion of gastric cancer cells by directly targeting ρ‑associated protein kinase 1

New miRNA expression abnormalities in laryngeal squamous cell carcinoma

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