MiR-135b-5p and MiR-499a-3p Promote Cell Proliferation and Migration in Atherosclerosis by Directly Targeting MEF2C

Xu, Zhiliang; Han, Yeming; Liu, Jiying; Jiang, Fan; Hu, Huili; Yan, Wei; Liu, Qiji; Gong, Yaoqin; Li, Xi

doi:10.1038/srep12276

Cited by 79 publications

(78 citation statements)

References 46 publications

(75 reference statements)

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“…Deregulation of miRNAs has been found in many types of human cancers 151617. Recent studies have found that miRNAs also regulated the functions of VSMSCs 181920. As PDGF-bb treatment promotes VSMC proliferation and migration, changes in miRNA expression profiles by PDGF-bb may be of great interest to identify novel miRNAs, which may regulate proliferation and migration.…”

Section: Discussionmentioning

confidence: 99%

“…To date, the role of miR-379 in VSMC function has not been investigated. One study found that miR-379 levels are lower in circulating blood from patients with atherosclerotic coronary artery disease 18. In cancer studies, miR-379 was found to inhibit breast cancer cell proliferation by regulating cyclin B1 expression;21 Yamamoto reported that miR-379 inhibited cell growth of malignant pleural mesothelioma by targeting interleukin 18 23.…”

Section: Discussionmentioning

confidence: 99%

“…Increasing evidence has also demonstrated that aberrant expression of miRNAs is involved in the development and progression of many types of cancers, including colon cancer, liver cancer, gastric cancer, lung cancer, breast cancer, and so on 151617. Meanwhile, recent studies have found that miRNAs also regulate the functions of VSMCs: for example, Xu, et al18, demonstrated that miR-135b and miR-499a promote cell proliferation and migration in the atherosclerosis by directly targeting myocyte enhance factor 2C; Liu, et al19, reported that miR-1 regulates the proliferation of VSMCs by targeting insulin-like growth factor-1 (IGF-1); and Xie, et al20, showed that miR-599 inhibits VSMCs proliferation and migration by targeting transforming growth factor beta 2 20. However, the role of miRNAs in VSMC functions has not been fully explored.…”

Section: Introductionmentioning

confidence: 99%

“…Xu, et al18, reported that miR-379 was down-regulated in circulating blood from patients with atherosclerotic coronary artery disease. miR-379 has also been found to inhibit cell growth in certain types of cancers including breast cancer, malignant mesothelioma, and liver cancer 212223.…”

Section: Introductionmentioning

confidence: 99%

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miR-379 Inhibits Cell Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells by Targeting Insulin-Like Factor-1

Wang

Zhang

et al. 2017

Yonsei Med J

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PurposeMicroRNAs are small non-coding RNAs that play important roles in vascular smooth muscle cell (VSMC) function. This study investigated the role of miR-379 on proliferation, invasion, and migration of VSMCs and explored underlying mechanisms thereof.Materials and MethodsMicroRNA, mRNA, and protein levels were determined by quantitative real-time PCR and western blot. The proliferative, invasive, and migratory abilities of VSMCs were measured by CCK-8, invasion, and wound healing assay, respectively. Luciferase reporter assay was used to confirm the target of miR-379.ResultsPlatelet-derived growth factor-bb was found to promote cell proliferation and suppress miR-379 expression in VSMCs. Functional assays demonstrated that miR-379 inhibited cell proliferation, cell invasion, and migration. Flow cytometry results further showed that miR-379 induced apoptosis in VSMCs. TargetScan analysis and luciferase report assay confirmed that insulin-like growth factor-1 (IGF-1) 3'UTR is a direct target of miR-379, and mRNA and protein levels of miR-379 and IGF-1 were inversely correlated. Rescue experiments showed that enforced expression of IGF-1 sufficiently overcomes the inhibitory effect of miR-379 on cell proliferation, invasion, and migration in VSMCs.ConclusionOur results suggest that miR-379 plays an important role in regulating VSMCs proliferation, invasion, and migration by targeting IGF-1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

miR-379 Inhibits Cell Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells by Targeting Insulin-Like Factor-1

Wang

Zhang

et al. 2017

Yonsei Med J

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“…Li et al (9) reported that increased miR-155 relieves chronic inflammation in AS. Xu et al (10) suggested that miR-135b-5p and miR-499a-3p promoted cell proliferation and migration in AS. Zhang et al (11) demonstrated that miR-26a prevented endothelial cell apoptosis under AS conditions.…”

Section: Introductionmentioning

confidence: 99%

Blockade of 146b‑5p promotes inflammation in atherosclerosis‑associated foam cell formation by targeting TRAF6

Lin

2017

Exp Ther Med

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Abstract. Atherosclerosis (AS) is a chronic inflammation in response to lipid accumulation. Increasing evidence has demonstrated that numerous microRNAs (miRs) have critical roles in inflammatory responses. A previous study suggested that miR-146b-5p is possibly associated with AS; however, its exact role has remained largely elusive. The present study aimed to investigate the potential role of miR-146b-5p in AS and to explore the underlying mechanism. Fist, the levels of miR-146b-5p were determined in foam cells and clinical specimens from patients with AS by reverse-transcription quantitative PCR. The role of miR-146b-5p in AS was then investigated by using miR-146b-5p inhibitor. The results demonstrated that the expression levels of miR-146b-5p were elevated in the lesions of patients with AS. In addition, the levels of miR-146b-5p in THP-1 cells stimulated with phorbol 12-myristate 13-acetate (100 nM) to induce their differentiation into macrophages were dose-and time-dependently elevated by oxidized low-density lipoprotein treatment applied for inducing foam cell formation. miR-146b-5p was also revealed to directly target tumor necrosis factor receptor-associated factor 6 (TRAF6), which functions as a signal transducer in the nuclear factor-κB (NF-κB) pathway. Furthermore, the present study reported for the first time that miR-146b-5p inhibition promotes the inflammatory response and enhances lipid uptake during foam cell formation. In conclusion, miR-146b-5p inhibition promoted chronic inflammation and had a detrimental role during AS-associated foam cell formation by targeting TRAF6.

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Downregulation of microRNA‐135b promotes atherosclerotic plaque stabilization in atherosclerotic mice by upregulating erythropoietin receptor

Liu

et al. 2019

IUBMB Life

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Atherosclerotic plaque rupture is an important pathophysiologic mechanism of acute coronary syndrome. Emerging microRNAs (miRNAs) have been implicated in the atherosclerotic plaque formation and macrophage autophagy during the development of atherosclerosis (AS). Hence, this study was conducted to explore the role microRNA‐135b (miR‐135b) in macrophages and atherosclerotic plaque in mouse models of AS. The expression of miR‐135b and erythropoietin receptor (EPOR) was altered in atherosclerotic mice to clarify their effect on inflammation, cell activities of aortic tissues, and macrophage autophagy. The obtained findings unraveled that miR‐135b was upregulated and EPOR was downregulated in atherosclerotic mice. Upregulated miR‐135b expression promoted cell apoptosis and inflammation, along with inhibited cell proliferation and decreased macrophage autophagy. Notably, miR‐135 was validated to target EPOR and activate the PI3K/Akt signaling pathway. Moreover, miR‐135b inhibition attenuated inflammation, atherosclerotic plaque development, and promoted macrophage autophagy. Besides, the effect of miR‐135b inhibition was reversed in response to EPOR silencing. Taken conjointly, the study revealed that inhibition of miR‐135b promoted macrophage autophagy and atherosclerotic plaque stabilization in atherosclerotic mice by inactivating the PI3K/Akt signaling pathway and upregulating EPOR.

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MiR-135b-5p and MiR-499a-3p Promote Cell Proliferation and Migration in Atherosclerosis by Directly Targeting MEF2C

Cited by 79 publications

References 46 publications

miR-379 Inhibits Cell Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells by Targeting Insulin-Like Factor-1

miR-379 Inhibits Cell Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells by Targeting Insulin-Like Factor-1

Blockade of 146b‑5p promotes inflammation in atherosclerosis‑associated foam cell formation by targeting TRAF6

Downregulation of microRNA‐135b promotes atherosclerotic plaque stabilization in atherosclerotic mice by upregulating erythropoietin receptor

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