miR-133 involves in lung adenocarcinoma cell metastasis by targeting FLOT2

Wei, Guangxia; Xu, Yahuan; Peng, Tao; Yan, Jie

doi:10.1080/21691401.2017.1324467

Cited by 17 publications

(14 citation statements)

References 26 publications

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“…Results of prediction from bioinformatics website confirmed that FLOT2 was in targeting relationship with miR‐351. Similarly, a former paper implicated that in the invasion and migration of lung adenocarcinoma, miR‐133 targeted with FLOT2 . Flotillins, also named as reggie proteins, consist of FLOT1 and FLOT2, two commonly expressed and highly conserved proteins .…”

Section: Discussionmentioning

confidence: 89%

MicroRNA‐351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus

Chen

Liu

Peng

2019

J Cellular Molecular Medi

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Gestational diabetes mellitus (GDM) is known as different degree glucose intolerance that is initially identified during pregnancy. MicroRNAs (miRs) may be a potential candidate for treatment of GDM. Herein, we suggested that miR‐351 could be an inhibitor in the progression of GDM via the phosphoinositide 3‐kinase/protein kinase B (PI3K/AKT) pathway. Microarray analysis was used to identify differentially expressed genes and predict miRs regulating flotillin 2 (FLOT2). Target relationship between miR‐351 and FLOT2 was verified. Gestational diabetes mellitus mice were treated with a series of mimic, inhibitor and small interfering RNA to explore the effect of miR‐351 on insulin resistance (IR), cell apoptosis in pancreatic tissues and liver gluconeogenesis through evaluating GDM‐related biochemical indexes, as well as expression of miR‐351, FLOT2, PI3K/AKT pathway‐, IR‐ and liver gluconeogenesis‐related genes. MiR‐351 and FLOT2 were reported to be involved in GDM. FLOT2 was the target gene of miR‐351. Gestational diabetes mellitus mice exhibited IR and liver gluconeogenesis, up‐regulated FLOT2, activated PI3K/AKT pathway and down‐regulated miR‐351 in liver tissues. Additionally, miR‐351 overexpression and FLOT2 silencing decreased the levels of FLOT2, phosphoenolpyruvate carboxykinase, glucose‐6‐phosphatase, fasting blood glucose, fasting insulin, total cholesterol, triglyceride, glyeosylated haemoglobin and homeostasis model of assessment for IR index (HOMA‐IR), extent of PI3K and AKT phosphorylation, yet increased the levels of HOMA for islet β‐cell function, HOMA for insulin sensitivity index and glucose transporter 2 expression, indicating reduced cell apoptosis in pancreatic tissues and alleviated IR and liver gluconeogenesis. Our results reveal that up‐regulation of miR‐351 protects against IR and liver gluconeogenesis by repressing the PI3K/AKT pathway through regulating FLOT2 in GDM mice, which identifies miR‐351 as a potential therapeutic target for the clinical management of GDM.

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Section: Discussionmentioning

confidence: 89%

MicroRNA‐351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus

Chen

Liu

Peng

2019

J Cellular Molecular Medi

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“…Knockdown of Flot-2 inhibits the growth and invasion of human esophageal squamous cell carcinoma [32]. Targeting FLOT2 via the Akt signaling pathway, miR-133 and miR-485 inhibits lung metastasis [33,34]. miR-34a inhibits melanoma cell proliferation and metastasis by directly targeting the FLOT2 gene [35].…”

Section: Discussionmentioning

confidence: 99%

The study of mechanism of miR-34c-5p targeting FLOT2 to regulate proliferation, migration and invasion of osteosarcoma cells

Wang

Tang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Objective: Osteosarcoma is one of the most common malignancies in children and adolescents. Studies have shown that miR-34c-5p is involved in the progression of various cancers. To explore the effects of miR-34c-5p on the proliferation, migration and invasion of osteosarcoma cells and its potential mechanism. Methods: qRT-PCR was used to detect the expression levels of miR-34c-5p and FLOT2 mRNA in osteosarcoma tissues and cells. Western Blot was used to detect protein expression. MTT assay used to detect cell viability. Transwell was used to detect cell migration and invasion in each group. Dual luciferase reporter gene assay was used to detect luciferase activity. Results: The expression of miR-34c-5pwas significantly decreased in osteosarcoma tissues and cells and the expression level of FLOT2 mRNA was significantly increased. Overexpression of miR-34c-5p and inhibition of FLOT2 inhibited the proliferation, migration and invasion of osteosarcoma cells and inhibited the expression of Cyclin D1, MMP-2 and MMP-9 proteins and promoted the expression of p21 protein. miR-34c-5p targeted to regulate the expression of FLOT2. Overexpression of FLOT2 reversed the inhibitory effect of miR-34c-5p overexpression on proliferation, migration and invasion of osteosarcoma cell lines. Conclusion: miR-34c-5p can inhibit the proliferation, migration and invasion of osteosarcoma cells. The mechanism may be related to targeting FLOT2, which will provide a new target for the prevention and treatment of osteosarcoma.

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“…The Flotillin family contains two homologous isoforms, flotillin1 (FLOT1) and 2 (FLOT2), which play essential physical roles in various cellular processes such as adhesion, reorganization of the actin cytoskeleton, endocytosis, phagocytosis, and transduction of cellular signals. Flotillin-1 oligomerizes to build microdomain scaffolds that are involved in molecular sorting [21][22][23], cytoskeletal dynamics [24], clathrinindependent endocytic pathways [25][26][27] and phagosome trafficking [14][15][16][17][18]. However, it also promotes cell proliferation [22] and T cell activation [22,23].…”

Section: Introductionmentioning

confidence: 99%

“…Butz H et al proved that validated CAV1 and FLOT1 as miR-124-3p targets [26]. Huang et al showed that FLOT2 identified as a direct target of miR-133 in human lung adenocarcinoma [27]. And Wang et al identified Flot2 as a direct target of miR-802 in PCa cells [28].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-485-5p suppresses the proliferation, migration and invasion of small cell lung cancer cells by targeting flotillin-2

Gao

Wang

et al. 2019

Bioengineered

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2019) MicroRNA-485-5p suppresses the proliferation, migration and invasion of small cell lung cancer cells by targeting flotillin-2, ABSTRACTThis study is aimed to elucidate the mechanisms underlying the role of miR-485-5p in small cell lung cancer (SCLC). The expression of miR-485-5p were quantified with real time quantitative PCR and it was found that the level of miR-485-5p was lower in SCLC tissues than normal tissues. In cultured SCLC cell lines, overexpression of miR-485-5p reduced cell proliferation, migration, and invasion in vitro, whereas knockdown of miR-485-5p performed contrary. FLOT2 expression was obviously upregulated and negatively correlated with miR-485-5p expression level in SCLC tissues. Overexpression of miR-485-5p significantly inhibited the protein expression of flotillin-2 (FLOT2) in cultured SCLC cells. Luciferase reporter assay confirmed that FLOT2 was a direct target of miR-485-5p in SCLC cells. It is concluded that miR-485-5p, as a tumor suppressor, inhibits the growth and metastasis in SCLC by targeting FLOT2. Upregulation of miR-485-5p expression may be an attractive strategy for SCLC therapy. ARTICLE HISTORY

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miR-133 involves in lung adenocarcinoma cell metastasis by targeting FLOT2

Abstract: Our study revealed the mechanism that miR-133 suppresses lung adenocarcinoma metastasis by targeting FLOT2 via Akt signalling pathway, implicating a potential prognostic biomarker and therapeutic target for lung adenocarcinoma treatment.

Cited by 17 publications

References 26 publications

MicroRNA‐351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus

MicroRNA‐351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus

The study of mechanism of miR-34c-5p targeting FLOT2 to regulate proliferation, migration and invasion of osteosarcoma cells

MicroRNA-485-5p suppresses the proliferation, migration and invasion of small cell lung cancer cells by targeting flotillin-2

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