miR-133 inhibits pituitary tumor cell migration and invasion via down-regulating FOXC1 expression

Wang, D S; Zhang, H Q; Zhang, B; Yuan, Zhiyao; Yu, Zhongxiang; Yang, Tingting; Liu, Y; Jia, Xiaojing

doi:10.4238/gmr.15017453

Cited by 32 publications

(28 citation statements)

References 28 publications

(29 reference statements)

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“…miRNAs function as key regulators of gene expression via binding to the 3'-untranslated region (UTR) of the target mRNAs, and consequently inducing the degradation or translation inhibition of the mRNAs (5,7,8). Notably, emerging evidence has illustrated the critical roles of miRNAs in the pathogenesis of different cancer types (9)(10)(11)(12)(13)(14)(15)(16). For example, miR-106b targeted the tumor suppressor phosphatase and tensin homolog and promoted the growth of pituitary cancer cells (17).…”

Section: Introductionmentioning

confidence: 99%

“…For example, miR-106b targeted the tumor suppressor phosphatase and tensin homolog and promoted the growth of pituitary cancer cells (17). Another study reported that miR-133 suppressed the migration and invasion of pituitary tumor cells by downregulating the expression of forkhead box C1 (14). Recently, a growing body of evidence has suggested that miRNAs regulate the progression of cancer by affecting the metabolism of cancer cells, particularly aerobic glycolysis (18)(19)(20)(21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of glucose‑6‑phosphate dehydrogenase by microRNA‑1 inhibits the growth of pituitary tumor cells

Yang

Ding

et al. 2018

Oncol Rep

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Pituitary tumors are generally intracranial neoplasms with high incidence and mortality rates. The investigation of novel factors involved in the tumorigenesis of pituitary tumors and the characterization of the underlying molecular mechanisms is urgently required for the diagnosis and treatment of pituitary tumors. Accumulating evidence has indicated that microRNAs (miRs) serve important roles in the initiation and progression of cancer. The present study found that miR-1 was significantly downregulated in pituitary tumor tissues upon reverse transcription-quantitative polymerase chain reaction analysis. Decreased expression of miR-1 was associated with the progression and worse prognosis of patients with pituitary tumors. The MTT assay showed that overexpression of miR-1 significantly suppressed proliferation. Highly expressed miR-1 promoted the apoptosis of pituitary tumor cells upon fluorescence-activated cell sorting analysis. Further molecular study revealed that glucose-6-phosphate dehydrogenase (G6PD), the first and rate-limiting enzyme of the pentose phosphate pathway (PPP), was one of the targets of miR-1. Western blot assays showed that overexpression of miR-1 significantly decreased the protein level of G6PD in pituitary tumor cells without changing the mRNA level of G6PD. Consequently, oxidative PPP flux analysis revealed that suppression of G6PD by miR-1 decreased the production of nicotinamide adenine dinucleotide phosphate and the glycolysis of pituitary cancer cells. Restoration of the expression of G6PD significantly reversed the inhibitory effect of miR-1 on the PPP and the growth of pituitary tumor cells. Collectively, the present results uncovered the critical involvement of miR-1 in pituitary tumors, indicating that miR-1 is a potential therapeutic target for the treatment of pituitary tumors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Downregulation of glucose‑6‑phosphate dehydrogenase by microRNA‑1 inhibits the growth of pituitary tumor cells

Yang

Ding

et al. 2018

Oncol Rep

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“…For instance, Chang et al [] reported that miR‐494 exacerbated OSCC via targeting Bmi1 and ADAM10 ; Deng and Liu [] argued that miR‐506 suppressed the viability and invasion of OSCC cells by targeting GATA6 . Meanwhile, Wang et al [] insisted that miR‐133 could function in a variety of metastatic cancers as a tumor suppressor and demonstrated that miR‐133 inhibited cell migration and invasion ability by directly targeting FOXC1 in pituitary adenoma. Xiao et al [] confirmed that the re‐expression of miR‐133 suppressed the migration and invasion of lung cancer cells.…”

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confidence: 99%

MiR‐133a‐3p Inhibits Oral Squamous Cell Carcinoma (OSCC) Proliferation and Invasion by Suppressing COL1A1

Lin

Feng-qi

et al. 2017

J of Cellular Biochemistry

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The aim of our study was to investigate the effects of miR-133a-3p on human oral squamous cell carcinoma (OSCC) cells by regulating gene COL1A1. OSCC tissues, adjacent tongue epithelial tissues, the immortalized oral epithelial cell line HIOEC, and OSCC cell lines (CAL-27, TCA-8113, SCC-4, SCC-9, and SCC-15) were used in this research. Quantitative real-time PCR (RT-qPCR) was employed to determine the expression of miR-133a-3p and COL1A1. Dual luciferase reporter gene assay and Western blot were applied to verify the binding relationship between miR-133a-3p and COL1A1. Functional assays were also conducted in this study, including CCK-8 assay, colony formation assay, flow cytometry analysis as well as Transwell assay. MiR-133a-3p was found low-expressed both in OSCC tissues and cells lines compared with normal tissues and cell line, respectively, whereas COL1A1 was just the opposite. The over-expression of miR-133a-3p or the down-regulation of COL1A1 suppressed the proliferation, invasion, and mitosis of OSCC cells, whereas simultaneous down-regulation of miR-133a-3p and up-regulation of COL1A1 led to no significant alteration of cell activities. MiR-133a-3p could inhibit the proliferation and migration of OSCC cells through directly targeting COL1A1 and reducing its expression.

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“…There were 41 downregulated and 80 upregulated miRNAs identified in the D_T library compared with the B_T library. 28 Similarly, we also identified these relative miRNAs family members in this study. It had been reported that gga-miR-181a was involved in Marek's disease (MD) lymphomagenesis.…”

Section: Discussionmentioning

confidence: 67%

Transcriptom analysis revealed regulation of dexamethasone induced microRNAs in chicken thymus

Zhou

Tian

Zhang

et al. 2018

J of Cellular Biochemistry

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Stress‐induced immunosuppression is one of the serious threats to the poultry industry, especially obvious for young chicken. However, the molecular mechanism of stress‐induced immunosuppression has not been clear in chicken. Here, we established an immunosuppression model of 7‐day‐old chickens with injecting dexamethasone (Dex) to analyze the molecular regulation in the chicken thymus. The microRNAs (miRNAs) transcripts profiles of thymuses from the model and control group were identified by the Solexa sequencing technology. The results showed 121 significantly differently expressed (SDE) miRNAs, including 119 known and two novel miRNAs (novel‐58 and novel‐350). A total of 391 target genes of the SDE miRNAs were predicted and annotated. We verified the potential negative correlation between gga‐miR‐103‐3p and TGM2 by quantitative real‐time polymerase chain reaction (qRT‐PCR), as well as between novel‐350 and PCBD2, and the results were positive. Gene ontology (GO) enrichment analysis showed that there was 298 significant enrichment GO terms, in which 31 were related to immune or stress, such as lymphocyte apoptotic process and response to stress. KEGG pathway analysis suggested that the SDE miRNAs were involved in autophagy regulation, cytokine‐cytokine receptor interaction, Toll‐like receptor signaling pathway, Jak‐STAT signaling pathway, and so on (although not significantly enriched). In these immune signaling pathways, the SDE miRNAs (such as gga‐miR‐2954, gga‐miR‐146b‐3p, gga‐miR‐106‐3p, and gga‐miR‐214) and the predicted target genes (such as IL11Ra, CSF3R, IFNGR1, CNTF, and MAP2K2) might affect the thymus immune function of chicken. The above results would provide a basis for uncovering the molecular regulation mechanism of immunosuppression in poultry.

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miR-133 inhibits pituitary tumor cell migration and invasion via down-regulating FOXC1 expression

Cited by 32 publications

References 28 publications

Downregulation of glucose‑6‑phosphate dehydrogenase by microRNA‑1 inhibits the growth of pituitary tumor cells

Downregulation of glucose‑6‑phosphate dehydrogenase by microRNA‑1 inhibits the growth of pituitary tumor cells

MiR‐133a‐3p Inhibits Oral Squamous Cell Carcinoma (OSCC) Proliferation and Invasion by Suppressing COL1A1

Transcriptom analysis revealed regulation of dexamethasone induced microRNAs in chicken thymus

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