miR-130b-3p is high-expressed in polycystic ovarian syndrome and promotes granulosa cell proliferation by targeting SMAD4

Bao, Dongqin; Li, Mingan; Zhou, Dansha; Zhuang, Chaohui; Ge, Zhijuan; Qian, Wei; Zhang, Limin

doi:10.1016/j.jsbmb.2021.105844

Cited by 11 publications

(6 citation statements)

References 43 publications

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“…MicroRNAs (miRNAs) are ~22nt long small and non-coding RNAs that directly target the 3′-UTR region of mRNAs, thus affecting mRNA transcription and translation [ 5 , 6 ]. miRNAs have been reported to have essential functions in metabolic disorders, including PCOS [ 7 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

microRNA-194 is increased in polycystic ovary syndrome granulosa cell and induce KGN cells apoptosis by direct targeting heparin-binding EGF-like growth factor

Lin

et al. 2021

Reprod Biol Endocrinol

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Background Polycystic ovary syndrome (PCOS) is an endocrine-related follicular developmental disorder that affects 50 %-70 % of reproductive-aged women diagnosed with ovulation-related infertility. Abnormal proliferation and apoptosis of granulosa cells (GCs) are thought to be the critical factors leading to abnormal maturation of follicles. It has been shown that microRNAs (miRNAs) exert a significant influence in the pathogenesis of PCOS; however, the relationship between miRNA, PCOS, and GC apoptosis is not entirely understood. Methods To clarify the effect of miR-194 in PCOS, CCK-8, Ki67 staining, AO/EB, and flow cytometry assays were used to assess cell growth, proliferation, and apoptosis in KGN cells, which were artificially stimulated to overexpress miR-194. Luciferase reporter assays and rescue experiments were used to elucidate the mechanism underlying miR-194 in PCOS. Results miR-194 expression was significantly up-regulated in rat models of PCOS and the ovarian GCs of PCOS patients. miR-194 suppression promoted KGN cell growth and proliferation. miR-194 overexpression also induced cell apoptosis, while miR-194 downregulation had an opposite effect. Furthermore, up-regulating heparin-binding EGF-like growth factor (HB-EGF) expression rescued the pro-apoptotic effects of miR-194 upregulation on KGN cells. Conclusions miR-194 is increased in PCOS granulosa cell and may function as a novel biomarker and therapeutic target for KGN cells via HB-EGF regulation.

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Section: Introductionmentioning

confidence: 99%

microRNA-194 is increased in polycystic ovary syndrome granulosa cell and induce KGN cells apoptosis by direct targeting heparin-binding EGF-like growth factor

Lin

et al. 2021

Reprod Biol Endocrinol

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“…The reversal of hypoxia can cause the decrease of SIRT1 activity in mitochondria and during hypoxia. [48][49][50] The ovarian GC of PCOS patients show abnormal proliferation, which can be reversed by reducing cell proliferation and inhibiting apoptosis with inhibitors. [51] Nitric oxide (NO) plays a role in the physiology of the reproductive system, follicular development and selection, is associated with angiogenic events, play a regulatory role in steroid production in ovarian cells by stimulating cyclooxygenase-2 activity, NO induces increased production of prostaglandin E2, which appears to be a common ovulation mechanism, NO level is negatively correlated with IR in PCOS patients, and the effects of NO and IR are mutually reinforcing, many of the effects of insulin depend on NO, which is an effector in insulin information transduction pathway.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of modified Huanglian Wendan decoction in the treatment of polycystic ovary syndrome

Yang

2023

Medicine

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To investigate the mechanism of modified Huanglian Wendan decoction in the intervention of polycystic ovary syndrome (PCOS) by network pharmacology and molecular docking. The ingredients and targets of modified Huanglian Wendan decoction were retrieved from the traditional Chinese medicine Systems Pharmacology database. Related targets of PCOS were screened by Comparative Toxicogenomics Database database. Cytoscape 3.7.2 ( https://cytoscape.org/ ) was used to draw the target network diagram of “traditional Chinese medicine - ingredient - PCOS,” STRING database was used to construct the target protein interaction network. NCA tool of Cystoscape 3.7.2 was used to carried out topology analysis on PPI network, core components and key targets were obtained. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis were carried out for the intersection targets by David database. AutoDockTools 1.5.6 software ( https://autodock.scripps.edu/ ) was used to conduct molecular docking verification of key components and key targets. Ninety-one ingredients of the modified Huanglian Wendan decoction and 23,075 diseases targets were obtained, 155 Intersection targets of the drug and disease were obtained by R language, Veen plot was drawn. Gene ontology enrichment analysis obtained 432 biological processes, 67 cell components, 106 molecular functions. Fifty-four Kyoto encyclopedia of genes and genomes enrichment pathways ( P < .05) including tumor necrosis factor, hypoxia-induced factors-1, calcium, and drug metabolism-cytochrome P450 signaling pathway. Molecular docking showed quercetin, luteolin, kaempferol, and baicalein were stable in docking with core targets. Network pharmacology and molecular docking were used to preliminarily study the mechanism of action of modified Huanglian Wendan decoction in the treatment of PCOS, which laid foundation for future experimental research and clinical application.

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“…BMP4 treatment suppressed androgen synthesis in thecal cells and promoted estrogen production in granulosa cells by regulating the expression of steroidogenic enzymes, including CYP11A, HSD3B2, CYP17A1, and CYP19A1. BMP4 levels are significantly decreased in hyperandrogenism [ 218 , 219 ]. The role of miR-29a in the regulation of the inflammatory background via AR needs clarification.…”

Section: Epigenetic Modulation Of Androgenic Activity In Response To ...mentioning

confidence: 99%

Modulation of the Inflammatory Response in Polycystic Ovary Syndrome (PCOS)—Searching for Epigenetic Factors

Szukiewicz

Trojanowski

Kociszewska

et al. 2022

IJMS

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Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. Despite its incidence, the syndrome is poorly understood and remains underdiagnosed, and female patients are diagnosed with a delay. The heterogenous nature of this complex disorder results from the combined occurrence of genetic, environmental, endocrine, and behavioral factors. Primary clinical manifestations of PCOS are derived from the excess of androgens (anovulation, polycystic ovary morphology, lack of or scanty, irregular menstrual periods, acne and hirsutism), whereas the secondary manifestations include multiple metabolic, cardiovascular, and psychological disorders. Dietary and lifestyle factors play important roles in the development and course of PCOS, which suggests strong epigenetic and environmental influences. Many studies have shown a strong association between PCOS and chronic, low-grade inflammation both in the ovarian tissue and throughout the body. In the vast majority of PCOS patients, elevated values of inflammatory markers or their gene markers have been reported. Development of the vicious cycle of the chronic inflammatory state in PCOS is additionally stimulated by hyperinsulinemia and obesity. Changes in DNA methylation, histone acetylation and noncoding RNA levels are presented in this review in the context of oxidative stress, reactive oxygen species, and inflammatory signaling in PCOS. Epigenetic modulation of androgenic activity in response to inflammatory signaling is also discussed.

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miR-130b-3p is high-expressed in polycystic ovarian syndrome and promotes granulosa cell proliferation by targeting SMAD4

Cited by 11 publications

References 43 publications

microRNA-194 is increased in polycystic ovary syndrome granulosa cell and induce KGN cells apoptosis by direct targeting heparin-binding EGF-like growth factor

microRNA-194 is increased in polycystic ovary syndrome granulosa cell and induce KGN cells apoptosis by direct targeting heparin-binding EGF-like growth factor

Molecular mechanism of modified Huanglian Wendan decoction in the treatment of polycystic ovary syndrome

Modulation of the Inflammatory Response in Polycystic Ovary Syndrome (PCOS)—Searching for Epigenetic Factors

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