miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway

Xie, Sijie; Jiang, Xiaoxue; Qin, Rushi; Song, Shuting; Lu, Yanan; Wang, Liyan; Chen, Yingjie; Lu, Dongdong

doi:10.1016/j.isci.2021.103271

Cited by 7 publications

(6 citation statements)

References 67 publications

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“…These hub genes displayed especially high expression in 3i/LAF‐PSCs, including HESX1 , important for self‐renewal and pluripotency maintenance, 28,29 as well as SOD2 , ESD , OSTC , NSA2 and PPM1K , which reduce oxidative stress, regulate cell cycle and promote cell survival, respectively (Figure S5D). 30–34 These results reflected the distinctive properties of 3i/LAF‐iPSCs in comparison to other PSCs. To summarize, 3i/LAF‐iPSCs exhibited striking similarities with pgEpiSCs.…”

Section: Resultsmentioning

confidence: 74%

Generation of stable integration‐free pig induced pluripotent stem cells under chemically defined culture condition

et al. 2023

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Genome integration‐free pig induced pluripotent stem cells (iPSCs) bring tremendous value in pre‐clinical testing of regenerative medicine, as well as conservation and exploitation of endangered or rare local pig idioplasmatic resources. However, due to a lack of appropriate culture medium, efficient induction and stable maintenance of pig iPSCs with practical value remains challenging. Here, we established an efficient induction system for exogenous gene‐independent iPSCs under chemically defined culture condition previously used for generation of stable pig pre‐gastrulation epiblast stem cells (pgEpiSCs). WNT suppression was found to play an essential role in establishment of exogenous gene‐independent iPSCs. Strikingly, stable integration‐free pig iPSCs could be established from pig somatic cells using episomal vectors in this culture condition. The iPSCs had pluripotency features and transcriptome characteristics approximating pgEpiSCs. More importantly, this induction system may be used to generate integration‐free iPSCs from elderly disabled rare local pig somatic cells and the iPSCs could be gene‐edited and used as donor cells for nuclear transfer. Our results provide novel insights into potential applications for genetic breeding of livestock species and pre‐clinical evaluation of regenerative medicine.

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Section: Resultsmentioning

confidence: 74%

Generation of stable integration‐free pig induced pluripotent stem cells under chemically defined culture condition

et al. 2023

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“…There is a close relationship between ER stress and oxidative stress in the pathogenesis of pre-eclampsia [72]: two types of intracellular stress are caused by ischaemia-reperfusion and hypoxia, and an unfolded protein response (UPR) in the case of the accumulation of misfolded proteins can activate some of the same intracellular inflammatory signaling pathways as oxidative stress (NF-κB and p38MAPK pathways). In the present study, we identified miR-1307-3p as being an early predictive marker of pre-eclampsia, having the potential to target methyltransferase protein 8 (METTL8), which in turn inhibits the expression of KDM3A/3B and reduces methylation on the ninth lysine of histone H3, therefore providing the binding ability of P300 and RNA pol II to the promoter region of endoplasmic reticulum effector protein CALR [76]. We found a decrease in the miR-1307-3p level in the first-trimester blood serum level of women with early or late PE (Table S4), which indicates the impaired functioning of the endoplasmic reticulum UPR-signaling even before the onset of clinical manifestations of PE.…”

Section: Discussionmentioning

confidence: 93%

Prediction of Early- and Late-Onset Pre-Eclampsia in the Preclinical Stage via Placenta-Specific Extracellular miRNA Profiling

Timofeeva

Fedorov

Sukhova

et al. 2023

IJMS

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Pre-eclampsia (PE) is one of the severe complications of pregnancy in 2–8% of all cases and is one of the leading causes of maternal and perinatal mortality. The fundamental role in the pathogenesis of PE is assigned to maternal and/or placental factors, whereby the combination and manifestation of which determines the time of onset of the clinical symptoms of PE (before or after 34 weeks of gestation) and their severity. It is known that the expression level of miRNAs, the regulators of signaling cascades in the cell, depends on gestational age. In the present study, we focused on the identification of the placenta-specific miRNAs that differentiate between early- and late-onset pre-eclampsia (ePE and lPE) throughout pregnancy, from the first to the third trimester. A total of 67 patients were analyzed using small RNA deep sequencing and real-time quantitative PCR, which resulted in a core list of miRNAs (let-7b-5p, let-7d-3p, let-7f-5p, let-7i-5p, miR-22-5p, miR-451a, miR-1246, miR-30e-5p, miR-20a-5p, miR-1307-3p, and miR-320e), which in certain combinations can predict ePE or lPE with 100% sensitivity and 84–100% specificity in the 1st trimester of pregnancy. According to the literature data, these miRNA predictors of PE control trophoblast proliferation, invasion, migration, syncytialization, the endoplasmic reticulum unfolded protein response, immune tolerance, angiogenesis, and vascular integrity. The simultaneous detection of let-7d-3p, miR-451a, and miR-1307-3p, resistant to the repeated freezing/thawing of blood serum samples, in combination with biochemical (b-hCG and PAPP-A) and ultrasound (UAPI) parameters, allowed us to develop a universal model for the prediction of ePE and lPE onset (FPR = 15.7% and FNR = 9.5%), which was validated using a test cohort of 48 patients and demonstrated false-positive results in 26.7% of cases and false negatives in 5.6% of cases. For comparison, the use of the generally accepted Astraia program in the analysis of the test cohort of patients led to worse results: FPR = 62.1% and FNR = 33.3%.

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“…miR-1307, a gene on human chromosome 10 ( 62 ), can regulate ovarian cancer resistance to chemotherapy and increase the proliferation of prostate cancer cells ( 63 , 64 ). miR-1307 suppresses HCC cell autophagy and accelerates the malignant progression of HCC through the Calr-OSTC endoplasmic reticulum protein folding pathway ( 65 ).…”

Section: Mirnas Involved In the Regulation Of Autophagy In Hccmentioning

confidence: 99%

Non‑coding RNAs: Role of miRNAs and lncRNAs in the regulation of autophagy in hepatocellular carcinoma (Review)

et al. 2023

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The term autophagy describes a process that supports nutrient cycling and metabolic adaptation that is accomplished via multistep lysosomal degradation. These activities modulate cell, tissue and internal environment stability, and can also affect the occurrence and development of cancer. Previous studies have mostly described autophagy as having dual effects in cancer, serving to limit tumorigenesis in the early stages of cancer, but promoting tumor progression in certain types of cancer. There have been indications in recent years that microRNAs (miRNAs/miRs) and long non-coding RNAs (lncRNAs), as types of non-coding RNAs, play major roles in the occurrence, invasion, development and drug resistance of hepatocellular carcinoma (HCC) and in the migration of HCC cells by governing HCC cell autophagy. Therefore, understanding which miRNAs and lncRNAs play such roles and the relevant molecular mechanisms is critical. The present review highlights the significant functions of miRNAs and lncRNAs in the regulation of autophagy in HCC and the relevant mechanisms, aiming to provide novel insight into HCC therapeutics. Contents 1. Introduction 2. miRNAs involved in the regulation of autophagy in HCC 3. lncRNAs involved in the regulation of autophagy in HCC 4. Mechanisms of miRNAs and lncRNAs in the regulation of autophagy in HCC 5. Conclusions and future perspectives

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miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway

Cited by 7 publications

References 67 publications

Generation of stable integration‐free pig induced pluripotent stem cells under chemically defined culture condition

Generation of stable integration‐free pig induced pluripotent stem cells under chemically defined culture condition

Prediction of Early- and Late-Onset Pre-Eclampsia in the Preclinical Stage via Placenta-Specific Extracellular miRNA Profiling

Non‑coding RNAs: Role of miRNAs and lncRNAs in the regulation of autophagy in hepatocellular carcinoma (Review)

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