miR-1290 promotes lung adenocarcinoma cell proliferation and invasion by targeting SOCS4

Xiao, Xianmin; Yang, Dan; Gong, Xue; Mo, Dongping; Pan, Shiyang; Xu, Jian

doi:10.18632/oncotarget.24046

Cited by 25 publications

(32 citation statements)

References 36 publications

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“…Among the top-regulated miRNAs was miR-210, known to respond to hypoxia and with many documented roles in cancer [23]. Here, we identified a new hypoxia-associated miRNA, miR-1290, which promotes cell migration and invasion of cancer cells [69,70]. In addition, EV miR-1290 was found to be a biomarker in ovarian carcinoma [71].…”

Section: Discussionmentioning

confidence: 88%

Hypoxia-Induced Adaptations of miRNomes and Proteomes in Melanoma Cells and Their Secreted Extracellular Vesicles

Walbrecq

Lecha

Gaigneaux

et al. 2020

Cancers

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Reduced levels of intratumoural oxygen are associated with hypoxia-induced pro-oncogenic events such as invasion, metabolic reprogramming, epithelial–mesenchymal transition, metastasis and resistance to therapy, all favouring cancer progression. Small extracellular vesicles (EV) shuttle various cargos (proteins, miRNAs, DNA and others). Tumour-derived EVs can be taken up by neighbouring or distant cells in the tumour microenvironment, thus facilitating intercellular communication. The quantity of extracellular vesicle secretion and their composition can vary with changing microenvironmental conditions and disease states. Here, we investigated in melanoma cells the influence of hypoxia on the content and number of secreted EVs. Whole miRNome and proteome profiling revealed distinct expression patterns in normoxic or hypoxic growth conditions. Apart from the well-known miR-210, we identified miR-1290 as a novel hypoxia-associated microRNA, which was highly abundant in hypoxic EVs. On the other hand, miR-23a-5p and -23b-5p were consistently downregulated in hypoxic conditions, while the protein levels of the miR-23a/b-5p-predicted target IPO11 were concomitantly upregulated. Furthermore, hypoxic melanoma EVs exhibit a signature consisting of six proteins (AKR7A2, DDX39B, EIF3C, FARSA, PRMT5, VARS), which were significantly associated with a poor prognosis for melanoma patients, indicating that proteins and/or miRNAs secreted by cancer cells may be exploited as biomarkers.

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Section: Discussionmentioning

confidence: 88%

Hypoxia-Induced Adaptations of miRNomes and Proteomes in Melanoma Cells and Their Secreted Extracellular Vesicles

Walbrecq

Lecha

Gaigneaux

et al. 2020

Cancers

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“…Here, we identified a novel role for miR-1290, a host miRNA found in human and ferret lung cells, but not in mouse or chicken cells. Previously, miR-1290 expression has been reported to be upregulated in non-small cell lung cancer (NSCLC), 25 and miR-1290 has been implicated as a driver of invasiveness and proliferation in NSCLC26, 27, 28 and lung adenocarcinoma 29 . We found that an IAV can upregulate miR-1290 expression to enhance IAV replication.…”

Section: Introductionmentioning

confidence: 73%

“…It has been reported that ALDH4A1 is a p53-inducible gene that is protective against cellular stresses, 39 but it is not yet known whether ALDH4A1 also plays a role in IAV infection and host species specificity. However, previous research on miR-1290 has mostly centered on its role in promoting cell proliferation and invasiveness of lung cancer,26, 27, 28, 29 and cellular and animal studies have shown that inhibition of miR-1290 can decrease tumor cell invasiveness and arrest xenograft tumor growth 27, 28. Considering that miR-1290 has been reported to be upregulated in NSCLC, 26 lung adenocarcinoma, 29 and other tumors, together with the fact that cancer patients appear to be at increased risk of influenza complications, further research into the impact of miR-1290 on IAV proliferation and virulence in patients with miR-1290-upregulated tumors may be warranted.…”

Section: Discussionmentioning

confidence: 99%

Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus

Huang

Chen

et al. 2019

Molecular Therapy - Nucleic Acids

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The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3′ UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication.

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“… 10 miR-1290 is also highly expressed in LUAD cells and promotes LUAD cell proliferation and invasion by targeting and down-regulating SOCS4. 11 miR-1827 is down-regulated in LUAD and functions as a tumor suppressor to inhibit the development of LUAD. 12 However, the mechanism of miR-196b-5p in LUAD is still unclear.…”

Section: Introductionmentioning

confidence: 99%

miR-196b-5p Promotes Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells via Targeting RSPO2

Xu¹,

Xu²

2020

CMAR

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Objective: To explore the biological role of miR-196b-5p/RSPO2 in the occurrence and development of lung adenocarcinoma (LUAD) and to provide a basis for finding new therapeutic targets for LUAD. Methods: Differentially expressed genes were analyzed based on LUAD microarray, and the target gene of the target miRNA was predicted. qRT-PCR was used to detect the expression levels of miR-196b-5p and RSPO2 mRNA in normal human bronchial epithelial cell line BEAS-2B and LUAD cell lines A549, NCI-H1792 and NCI-H226. Western blot was used to evaluate protein expression. Cell proliferative, migratory and invasive abilities were detected by CCK-8 and transwell assays. Dual-luciferase assay was conducted to verify the targeting relationship between miR-196b-5p and RSPO2. Results: The results of qRT-PCR showed that miR-196b-5p was significantly highly expressed in LUAD cells, and the expression level of its downstream target gene RSPO2 was significantly decreased. The results of CCK-8 and transwell assays exhibited that miR-196b-5p promoted proliferation, migration and invasion of LUAD cells, while RSPO2 inhibited the malignant progression of LUAD cells. Dual-luciferase assay confirmed the targeted binding relationship between miR-196b-5p and RSPO2. Overexpression of RSPO2 partially reversed the promotion of miR-196b-5p on proliferation, migration and invasion of LUAD cells. Conclusion: miR-196b-5p promoted proliferation, migration and invasion of LUAD cells by targeting and down-regulating RSPO2, which provided ideas for searching new targets for the diagnosis and treatment of LUAD.

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miR-1290 promotes lung adenocarcinoma cell proliferation and invasion by targeting SOCS4

Cited by 25 publications

References 36 publications

Hypoxia-Induced Adaptations of miRNomes and Proteomes in Melanoma Cells and Their Secreted Extracellular Vesicles

Hypoxia-Induced Adaptations of miRNomes and Proteomes in Melanoma Cells and Their Secreted Extracellular Vesicles

Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus

miR-196b-5p Promotes Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells via Targeting RSPO2

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