miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit

Abstract

Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with HOXC13 expression (Pearson’s R = −0.313, p < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted HOXC13. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as BCL2, WNT2, CCND1, and LEF1 (p < 0.01), and promoted the expression of SFRP2, TGF-β1, and FGF2 (p < 0.01), which was the same as the knockdown of HOXC13. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of HOXC13. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (p < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (p < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting HOXC13, and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans.

Melatonin’s effect on hair follicles in a goat (Capra hircus) animal model

“…Melatonin may be a potential treatment for androgenetic alopecia ( 12 ). Rats ( 3 ), mice ( 4 ), guinea pigs ( 5 ), rabbits ( 6 ), and dogs ( 7 ) are animal models for studying human hair follicles. Comparative studies on the impact of diacylglycerol O-acyltransferase 1 (DGAT1) on mouse and dog alopecia suggest that mice may be an especially sensitive species ( 41 ).…”

“…Alopecia (hair loss) due to endocrine disorders has always plagued humans ( 1 , 2 ). Animal models for studying hair follicles in humans are usually rats ( 3 ), mice ( 4 ), guinea pigs ( 5 ), rabbits ( 6 ), and dogs ( 7 ). In addition, the goat PLP2 ( proteolipid protein 2 ) gene expressed only in the inner root sheath, suggesting that it may be associated with alopecia ( 8 ).…”