MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1

Xiang, Xiaojun; Deng, Jun; Liu, Yawen; Wan, Luying; Mao, Feng; Chen, Jun; Xiong, Jianping

doi:10.1159/000430304

Cited by 71 publications

(49 citation statements)

References 37 publications

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“…It is interesting to note, that numerous genetic changes are relation to the invasion and metastasis of cancer cells [23, 24]. Hence, in search for key regulators in the metastatic process of GC is very meaningful.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of ARK5 Expression Suppresses Invasion and Metastasis of Gastric Cancer

Chen¹,

Liu²,

Xu³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Gastric cancer (GC) is a common and lethal malignancy, and AMP-activated protein kinase-related kinase 5 (ARK5) has been discovered to promote cancer metastasis in certain types of cancer. In this study, we explored the role of ARK5 in GC invasion and metastasis. Methods: ARK5 and epithelial-mesenchymal transition (EMT)-related markers were determined by immunohistochemistry and western blot in GC specimens. Other methods including stably transfected against ARK5 into SGC7901 and AGS cells, western blot, migration and invasion assays in vitro and nude mice tumorigenicity in vivo were also employed. Results: The results demonstrated that ARK5 expression was increased and positively correlated with metastasis, EMT-related markers and poor prognosis in patients with GC. Knockdown of ARK5 expression remarkably suppressed GC cells invasion and metastasis via regulating EMT, rather than proliferation in vitro and in vivo. And knockdown of ARK5 expression in GC cells resulted in the down-regulation of the mTOR/p70S6k signals, Slug and SIP1. Conclusion: The elevated ARK5 expression was closely associated with cancer metastasis and patient survival, and it seemed to function in GC cells migration and invasion via EMT alteration, together with the alteration of the mTOR/p70S6k signals, Slug and SIP1, thus providing a potential therapeutic target for GC.

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Section: Discussionmentioning

confidence: 99%

Knockdown of ARK5 Expression Suppresses Invasion and Metastasis of Gastric Cancer

Chen¹,

Liu²,

Xu³

et al. 2017

Cell Physiol Biochem

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“…The roles of miR-1271 in numerous types of cancers have previously been investigated. For example, in gastric cancer, miR-1271 inhibited cell proliferation, invasion and epithelial-mesenchymal transition (EMT) by targeting forkhead box Q1 (FOXQ1) (27). Additionally, in oral squamous cell carcinoma, miR-1271 inhibited cell growth and metastasis by targeting anaplastic lymphoma receptor tyrosine kinase (28).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑1271 inhibits cellular proliferation of hepatocellular carcinoma

et al. 2017

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Abstract. Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-associated mortality worldwide, particularly in China. MicroRNAs (miRs) serve important roles in the pathogenesis of HCC. The present study investigated the function of miR-1271 in HCC. The miR-1271 levels were analyzed by quantitative reverse transcription polymerase chain reaction. Cells growth was examined by MTT assay. Bioinformatics algorithms from TargetScanHuman were used to predict the target genes of miR-1271. The protein level was assayed by western blotting. miR-1271 demonstrated a lower expression level in HCC tissues. Upregulation of miR-1271 suppressed the growth of HepG-2 and Huh-7 cells and induced apoptosis of cells. Forkhead box Q1 (FOXQ1) was targeted by miR-1271. In conclusion, miR-1271 is a novel tumor suppressor that inhibits HCC proliferation and induces cellular apoptosis by targeting FOXQ1 in HCC. The results of the present study may provide a novel therapeutic target of HCC. IntroductionHepatocellular carcinoma (HCC) accounts for ~80% of all liver cancer cases, and is one of the most common causes of cancer-associated mortality worldwide (1,2). The prevalence rate of HCC in China is particularly high, but continues to increase in numerous western countries (1,3). Despite HCC being was one of the first cancers to be linked epidemiologically to a definite risk factor, the underlying mechanisms of HCC pathogenesis remain unclear (4). The risk factors for HCC vary by location. In China, Hepatitis B or Hepatitis C virus infections are the main risk factors (5). The survival rate of patients with HCC has been extended due to progress in liver transplantation and other treatments; however, the insensitivity of chemotherapeutic drugs, cancer recurrence and metastasis continue to contribute to a poor prognosis (6). Thus, the identification of therapeutic targets and translation of molecular studies of HCC into clinical practice is urgently required.MicroRNAs (miRNAs) are a class of small non-coding RNAs, which are ~22 nucleotides in length (7-9). A number of miRNAs have been revealed to be involved in the pathogenesis of HCC (10-26). The roles of miR-1271 in numerous types of cancers have previously been investigated. For example, in gastric cancer, miR-1271 inhibited cell proliferation, invasion and epithelial-mesenchymal transition (EMT) by targeting forkhead box Q1 (FOXQ1) (27). Additionally, in oral squamous cell carcinoma, miR-1271 inhibited cell growth and metastasis by targeting anaplastic lymphoma receptor tyrosine kinase (28). In HCC, a previous study demonstrated that miR-96, miR-129-1-3p, miR-1291, miR-1303 and miR-1271 differentially regulated Glypican-3 (GPC3) expression levels in HCC cells and that the upregulation of GPC3 was associated with a concomitant downregulation of its repressor miR-1271 (29). However, the roles served by miR-1271 in HCC remain unclear.The present study analyzed the expression level of miR-1271 in HCC tissues and determined the in vitro function of miR-1271. The aim of the ...

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“…Deregulation of miRNA expression has been identified in many human diseases, including cancers [6,7]. Some highly expressed miRNAs could function as oncogenes by repressing tumor suppressor genes, whereas low-expressed miRNAs could function as tumor suppressors by negatively regulating oncogenes.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic and Prognostic Value of Serum MicroRNA-206 in Patients with Gastric Cancer

Hou

Luo

2016

Cell Physiol Biochem

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Aims: Recent studies have demonstrated that microRNAs (miRNAs) can serve as useful biomarkers for human cancers. The aim of this study was to evaluate the expression level of serum miRNA-206 in patients with gastric cancer (GC) and investigate its diagnostic and prognostic value. Methods: Quantitative real-time PCR was performed to evaluate serum miRNA-206 levels in 150 GC patients and 150 healthy volunteers. The association between miRNA-206 expression and clinicopathological factors as well as patient's survival was analyzed. Receiver operator curve (ROC) analysis was carried out to assess the potential value of serum miRNA-206 for GC diagnosis. Results: Serum miRNA-206 was down-regulated in GC patients compared with healthy controls (P < 0.001). Decreased serum miRNA-206 expression was significantly associated with deep local invasion, positive lymph node metastasis, and advanced clinical stage. Serum miRNA-206 expression was found to be significantly up-regulated in paired post-operative samples and reduced in patients with GC recurrence. ROC curve analysis showed that serum miRNA-206 was a useful marker for GC diagnosis, and could discriminate between recurred and non-recurred patients. Multivariate Cox regression analysis confirmed low serum miRNA-206 expression as an independent unfavorable prognostic factor for both DFS and OS of GC patients. Conclusions: These results suggest that serum miRNA-206 might not only serve as a novel diagnostic biomarker for GC, but also predict cancer recurrence and patient's prognosis.

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MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1

Cited by 71 publications

References 37 publications

Knockdown of ARK5 Expression Suppresses Invasion and Metastasis of Gastric Cancer

Knockdown of ARK5 Expression Suppresses Invasion and Metastasis of Gastric Cancer

MicroRNA‑1271 inhibits cellular proliferation of hepatocellular carcinoma

Diagnostic and Prognostic Value of Serum MicroRNA-206 in Patients with Gastric Cancer

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