miR-126-5p targets Malate Dehydrogenase 1 in non-small cell lung carcinomas

Queiroz, André Lima; Zhang, Boxi; Comstock, Dawn E.; Hao, Yuqing; Eriksson, Matilda; Vakifahmetoglu-Norberg, Helin; Norberg, Erik

doi:10.1016/j.bbrc.2018.03.154

Cited by 23 publications

(15 citation statements)

References 23 publications

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“…15) On the other hand, previous studies have shown that HMGB1 level in serum of patients with AS was significantly increased. 21,22) Our study also showed that miR-126-5p was downregulated and HMGB1 was upregulated in peripheral blood of patients with AS, which were consistent with previous findings.…”

Section: Discussionsupporting

confidence: 91%

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Baicalin Suppresses the Proliferation and Migration of Ox-LDL-VSMCs in Atherosclerosis through Upregulating miR-126-5p

Chen

Pan

Sheng

et al. 2019

Biological & Pharmaceutical Bulletin

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Atherosclerosis (AS) is a chronic inflammatory disease threatening human health, and vascular smooth muscle cells (VSMCs) are involved in AS processes. Baicalin is a flavonoid compound, which has antiatherosclerotic effect. The aim of our study was to explore the molecular mechanism of baicalin on AS. The expression of miR-126-5p was measured in peripheral blood of AS patients and healthy control. We found miR-126-5p expression was decreased in AS. Then, high-mobility group box 1 (HMGB1) was verified as a target of miR-126-5p and its expression was increased in AS. Similarly, miR-126-5p and HMGB1 expression was downregulated and upregulated in oxidized low-density lipoprotein treated VSMCs (ox-LDL-VSMCs), respectively. Furthermore, baicalin upregulated miR-126-5p and downregulated HMGB1 expression. Functionally, baicalin significantly inhibited ox-LDL-VSMCs proliferation and migration, and miR-126-5p targets HMGB1 to enhance the inhibition induced by baicalin. Taken together, baicalin is able to prevent AS, which suppressed the proliferation and migration of ox-LDL-VSMCs through upregulating miR-126-5p by targeting HMGB1. These findings suggested that baicalin is an effective drug to alleviate AS, and miR-126-5p is a novel therapeutic target for AS.

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Section: Discussionsupporting

confidence: 91%

“…Then, we further found the expression of miR-126-5p was downregulated in ox-LDL-VSMCs. Previous studies on miR-126-5p has focused on tumors, such as non-small cell lung cancer, 23,24) giant cell tumor, 25,26) followed by Parkinson's disease. 27) In AS, miR-126 could decrease the AS progression through reducing the cytokine release.…”

Section: Discussionmentioning

confidence: 99%

Baicalin Suppresses the Proliferation and Migration of Ox-LDL-VSMCs in Atherosclerosis through Upregulating miR-126-5p

Chen

Pan

Sheng

et al. 2019

Biological & Pharmaceutical Bulletin

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“…Other important targets include miR-22 downregulation on ATP citrate lyase (ACLY), which allowed for ACLY-mediated lipogenesis and caused increased metastatic effects [197]. Enzymatic activity of MDH1 can be altered as well with miR-126-5p in NSCLC, and with greater doses initiated cell toxicity [198]. The role of non-coding RNAs as regulators in a variety of pathways makes them an important part of studying tumor initiation and progression.…”

Section: Role Of Non-coding Rnas and Metabolism In Lung Cancermentioning

confidence: 99%

Non-Coding RNAs in Lung Tumor Initiation and Progression

Santos

Moreno

Zhang

2020

IJMS

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Lung cancer is one of the deadliest forms of cancer affecting society today. Non-coding RNAs, such as microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), through the transcriptional, post-transcriptional, and epigenetic changes they impose, have been found to be dysregulated to affect lung cancer tumorigenesis and metastasis. This review will briefly summarize hallmarks involved in lung cancer initiation and progression. For initiation, these hallmarks include tumor initiating cells, immortalization, activation of oncogenes and inactivation of tumor suppressors. Hallmarks involved in lung cancer progression include metastasis and drug tolerance and resistance. The targeting of these hallmarks with non-coding RNAs can affect vital metabolic and cell signaling pathways, which as a result can potentially have a role in cancerous and pathological processes. By further understanding non-coding RNAs, researchers can work towards diagnoses and treatments to improve early detection and clinical response.

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“…MiR-126-3p can hamper the growth, migration and invasion of non-small cell lung cancer by targeting CCR1 [24]. Lima et al [25]reported that the expression of miR-126-5p hampered the enzyme activity of MDH1 and mitochondrial respiration in NSCLC cells, leading to cell death. The up-regulation of long-chain non-coding RNA MINCR promoted the growth of non-small cells in lung cancer by negatively regulating the miR-126 /SLC7A5 axis [26].…”

Section: Discussionmentioning

confidence: 99%

Prognostic values and prospective pathway signaling of miR-126 in non-small cell lung cancer: a study based on gene expression omnibus and bioinformatics analysis

Jiao

et al. 2020

Preprint

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Objective MiRNAs are considered to be crucial for NSCLC’s initiation and development. MiRNAs have been widely identified in NSCLC. However, the role of miR-126 in NSCLC has not been fully explained.Methods miR-126 Expression in NSCLC was evaluated by analyzing the common data sets in Gene Expression Omnibus(GEO) database and reviewing former thesis papers. Three mRNA datasets, GSE18842, GSE19804 and GSE101929, from GEO to indentify the differentially expressed genes (DEG). We prognosed the target genes of hsa-miR-126-5p using TargetScan and analyzed the gene overlap between the target genes of miR-126 and DEG in NSCLC. Subsequently, we analyzed Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. We used STRING and Cytoscape to construct a protein-protein interaction (PPI) network, and analyzed the influence of HUB gene on the prognosis of NSCLC.Results A common pattern of mir-126 downregulation in NSCLC was identified in the literature review. A total of 187 DEGs were identified, both NSCLC-related and miR-126-related. Many DEGs are extendedly enriched in cell membranes, signal receptor binding, and biological regulation. Among the 10 main Hub genes analyzed by PPI, 4 HUB genes (NCAP-G,MELK,KIAA0101,TPX2) were obviously related to the poor recuperation of NSCLC patients. When these genes highly expressed, survival rate of NSCLC patients was low. Furthermore, we identified the recessive miR-126-related genes that may be involved in NSCLC, such as TPX2, HMMR, and ANLN through network analysis.Conclusion this study suggests that mir-126 is radical for the biological processing of NSCLC.

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miR-126-5p targets Malate Dehydrogenase 1 in non-small cell lung carcinomas

Cited by 23 publications

References 23 publications

Baicalin Suppresses the Proliferation and Migration of Ox-LDL-VSMCs in Atherosclerosis through Upregulating miR-126-5p

Baicalin Suppresses the Proliferation and Migration of Ox-LDL-VSMCs in Atherosclerosis through Upregulating miR-126-5p

Non-Coding RNAs in Lung Tumor Initiation and Progression

Prognostic values and prospective pathway signaling of miR-126 in non-small cell lung cancer: a study based on gene expression omnibus and bioinformatics analysis

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