MiR‐125b inhibits cell biological progression of Ewing's sarcoma by suppressing the PI3K/Akt signalling pathway

Li, J.; You, Tianyan; Jing, Juehua

doi:10.1111/cpr.12093

Cited by 87 publications

(58 citation statements)

References 22 publications

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“…24,25 EMT is a multistage process that contributes to cancer cells with marked changes in cellular morphology, loss and remodeling of cell-cell and cell-matrix adhesions and gain of migratory and invasive capabilities. 26 In the past years, several signal pathways have been identified that are critical for EMT in cancer progression and metastasis.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: MicroRNA-23a enhances migration and invasion through PTEN in osteosarcoma

Tian

Wang

2015

Cancer Gene Ther

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To investigate the biological significance of abundant microRNA-23a (miR-23a) expression in osteosarcoma and its correlation with PTEN in the pathogenesis of osteosarcoma migration and invasion. The human osteosarcoma cell lines MG63, HOS58 and SaoS-2, and the human normal osteoblasts (hFOB1.19) were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum. Gene and protein levels of miR-23a and PTEN were examined to determine the molecular relationship between them in the pathogenesis of osteosarcoma. Inhibition of miR-23a effectively reduced migration and invasion of osteosarcoma cell lines. Bioinformatics and luciferase-reporter assay revealed that miR-23a specifically targeted the 3'-untranslational region of PTEN and regulated its expression. Downregulation of PTEN enhanced migration and invasion of osteosarcoma cell lines. Furthermore, in tumor tissues obtained from osteosarcoma patients, the expression of miR-23a was negatively correlated with PTEN and the high expression of miR-23a combined with low expression of PTEN might serve as a risk factor for cancer patients. Besides, miR-23a-mediated suppression of PTEN led to activation of AKT/ERK pathways and epithelial-mesenchymal transition (EMT) in osteosarcoma cells, and finally enhanced the activity of osteosarcoma cell proliferation and movement and promoted osteosarcoma xenograft tumor growth in mouse models. Our study showed that miR-23a, by downregulation of PTEN, enhanced migration and invasion in osteosarcoma cells.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: MicroRNA-23a enhances migration and invasion through PTEN in osteosarcoma

Tian

Wang

2015

Cancer Gene Ther

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“…Recent reports have already given more prominence to the oncogenic aspects of miR125b, claiming that serum miR-125b could be a novel biomarker for screening human tumors, including non-small-cell lung cancer and breast cancer (Wang et al, 2012;Yuxia et al, 2012). Our previous miR array analysis in ES tissue samples has demonstrated the diagnostic potential of miR-125b (Li et al, 2014). Therefore, it is possible that miR-125b could become a biomarker for the diagnosis of ES.…”

Section: Discussionmentioning

confidence: 93%

Circulating miR-125b as a biomarker of Ewing's sarcoma in Chinese children

Nie¹,

Ren²,

Ma³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Previous studies indicated that has an important role in the progression of Ewing's sarcoma (ES). The purpose of the current study was to examine expression changes of miR125b in the serum of ES patients and evaluate if the expression level of miR-125b could serve as a new biomarker for ES. This study was performed on patients who underwent surgical resection at our hospital between 2005 and 2013 after an initial diagnosis of ES. We measured serum miR-125b levels in 63 patients with ES and 126 healthy control patients using a real-time quantitative reverse transcriptase-PCR (qRT-PCR) method. Expression levels of serum miR-125b were distinctly decreased in ES patients when compared with healthy controls (P < 0.001). ES cases that had a poor response to chemotherapy presented a significant down-regulation of miR-125b (P = 0.001). The ROC curve showed that the serum miR-125b could serve as a valuable biomarker for differentiating ES patients from healthy controls with an AUC of 0.879 (95%CI = 0.817-0.924; P < 0.001). At a cut-off value of 2.203 for miR-125b, the sensitivity was 72.8% and the specificity was 87.2% in 19049-19056 (2015) discriminating ES from the controls. Our results indicate that serum miR125b may serve as a useful noninvasive biomarker for ES.

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“…Another recent study by Li et al showed that restored expression of miR-125b in Ewing's sarcoma cell line A673 markedly suppressed cell proliferation, migration and invasion, arrested cell cycle progression, and stimulated cell apoptosis [63]. They further found that upregulation of miR-125b diminished the expression of PIK3 catalytic subunit delta (PIK3CD), and that inhibition of miR-125b attenuated phospho-AKT activity and retarded the concomitant biologic progression of A673 cells [63]. These results suggest that miR-125b functions as a tumor suppressor by targeting the PI3K/Akt signaling pathway, and in vivo delivery of miR-125b may have a potential therapeutic benefit for OS patients.…”

Section: Phosphatidylinositol-3-kinase (Pi3k)/akt Pathway-related Mirnasmentioning

confidence: 98%

“…Mechanistically, miR-223 can directly target heat shock protein 90B1 (Hsp90B1) and thereby inhibit PI3K/Akt/mTOR signaling [62]. Another recent study by Li et al showed that restored expression of miR-125b in Ewing's sarcoma cell line A673 markedly suppressed cell proliferation, migration and invasion, arrested cell cycle progression, and stimulated cell apoptosis [63]. They further found that upregulation of miR-125b diminished the expression of PIK3 catalytic subunit delta (PIK3CD), and that inhibition of miR-125b attenuated phospho-AKT activity and retarded the concomitant biologic progression of A673 cells [63].…”

Section: Phosphatidylinositol-3-kinase (Pi3k)/akt Pathway-related Mirnasmentioning

confidence: 99%

MicroRNAs in osteosarcoma

Zhang

Yan

Wang

et al. 2015

Clinica Chimica Acta

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MiR‐125b inhibits cell biological progression of Ewing's sarcoma by suppressing the PI3K/Akt signalling pathway

Abstract: Collectively, these data suggest that miR-125b functions as a tumor suppressor by targeting the PI3K/Akt/mTOR signaling pathway, and may provide potential therapy strategy for ES patients by targeting miRNA expression.

Cited by 87 publications

References 22 publications

RETRACTED ARTICLE: MicroRNA-23a enhances migration and invasion through PTEN in osteosarcoma

RETRACTED ARTICLE: MicroRNA-23a enhances migration and invasion through PTEN in osteosarcoma

Circulating miR-125b as a biomarker of Ewing's sarcoma in Chinese children

MicroRNAs in osteosarcoma

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