miR-1225-5p Functions as a Tumor Suppressor in Osteosarcoma by Targeting Sox9

Zhang, Weifei; Wei, Liangchen; Sheng, Weibei; Kang, Bin; Wang, Deli; Zeng, Hui

doi:10.1089/dna.2019.5105

Cited by 30 publications

(32 citation statements)

References 21 publications

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“…Consequently, it is not surprising that a part of miRNAs contribute as tumor suppressors in glioblastoma. These miRNAs include miR-1225-5p, which has been reported to have anticancer impacts in a variety of cancers [18,19]. miR-1225-5p was downregulated in thyroid cancer, which inhibited the malignant phenotype of thyroid cancer cells by regulating SIRT3 [20].…”

Section: Introductionmentioning

confidence: 99%

“…miR-1225-5p was downregulated in thyroid cancer, which inhibited the malignant phenotype of thyroid cancer cells by regulating SIRT3 [20]. It also plays an anticancer role in osteosarcoma, and this function was partially reversed by Sox9 [18]. Importantly, Li et al found that miR-1225-5p involved in glioblastoma pathogenesis as a tumor suppressor [21].…”

Section: Introductionmentioning

confidence: 99%

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MiR-1225-5p acts as tumor suppressor in glioblastoma via targeting FNDC3B

Wang

Zhang

et al. 2020

Open Medicine

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This study attempted to research the molecular mechanism underlying the inhibitory role of miR-1225-5p in the malignant progression of glioblastoma. Bioinformatics analyses based on the gene expression omnibus (GEO) and Chinese glioma genome atlas (CGGA) databases showed that miR-1225-5p, as a favorable prognostic factor, was expressed at low levels in glioblastoma, and its expression was also related to WHO grade and age. The subsequent CCK-8 assay indicated that miR-1225-5p might prevent the malignant progression of glioblastoma, which was represented by that miR-1225-5p mimic reduced the viability of glioblastoma cells. Then, we predicted that FNDC3B might be a potential target gene of miR-1225-5p, and it was negatively correlated with the level of miR-1225-5p, which were confirmed by dual-luciferase reporter assay, qRT-PCR and western blot assays. Moreover, based on the analyses of the cancer genome atlas (TCGA), Oncomine and CGGA databases, FNDC3B was enriched in glioblastoma and high expression of FNDC3B led to poor prognosis. Finally, CCK8 and transwell experiments showed that the ability of miR-1225-5p to inhibit glioblastoma cell viability, invasion and migration was at least partially achieved by targeting FNDC3B. In general, these results revealed that the miR-1225-5p/FNDC3B axis contributes to inhibiting the malignant phenotype of glioblastoma cells, which lays a foundation for molecular diagnosis and treatment of glioblastoma.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR-1225-5p acts as tumor suppressor in glioblastoma via targeting FNDC3B

Wang

Zhang

et al. 2020

Open Medicine

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“…miR-486 can promote the cell proliferation and mobility of renal cell carcinoma and inhibit its apoptosis ( 31 ). Several miRNAs have also been identified as effective biomarkers for the prognosis and progression of OS ( 32 , 33 ). miR-1225-5p serves as a tumor suppressor in OS and inhibits the proliferation, migration and invasion of OS cells by targeting Sox9 ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

“…Several miRNAs have also been identified as effective biomarkers for the prognosis and progression of OS ( 32 , 33 ). miR-1225-5p serves as a tumor suppressor in OS and inhibits the proliferation, migration and invasion of OS cells by targeting Sox9 ( 32 ). miR-384 is downregulated in OS tissues and cells compared to normal controls and upregulates stem loop binding protein and promotes the growth and metastasis of OS ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

2020

Oncol Lett

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Osteosarcoma (OS) is a type of primary bone tumor, which is one of the leading causes of cancer-related death. MicroRNA (miR)-605 has been demonstrated to act as a prognostic biomarker and therapeutic target in various cancers, such as breast cancer and non-small cell lung cancer, but its function in OS remains unclear. The aim of the present study was to investigate the prognostic value of miR-605 in patients with OS by evaluating its expression levels and to explore the biological function of miR-605 in OS progression. For this purpose, tumor tissues and adjacent normal tissues were collected from OS patients, and the expression of miR-605 in the collected tissues and OS MG63, U2OS, HOS, and SAOS-2 cell lines was detected by quantitative real-time PCR. The prognostic value of miR-605 was evaluated by Kaplan-Meier survival curves and Cox regression analysis. The effects of miR-605 on OS cell proliferation, migration and invasion were analyzed by the CCK-8 and transwell assays, respectively. The results of the present study revealed that miR-605 was significantly downregulated in OS tissues compared with adjacent normal tissues, which was associated with the clinical stage and distant metastasis of patients. Additionally, the downregulation of miR-605 predicted the poor prognosis of patients with OS and served as an independent prognostic indicator. The downregulation of miR-605 enhanced cell proliferation, migration, and invasion of OS cells, which suggested that miR-605 may be involved in the progression of OS. The findings of the present study provide a new therapeutic target for the treatment of patients with OS.

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“…With the development of targeted and immune therapies, miRNAs have gradually attracted increasing attention (9,10), and emerging evidence has indicated that abnormally expressed miRNAs may be involved in the occurrence and development of different types of cancer, in addition to being closely associated with the prognosis of patients (11)(12)(13). At present, it has been confirmed that miR-1225-5p serves a role in several types of cancers (14)(15)(16)(17). For example, the downregulated expression levels of miR-1225-5p in thyroid cancer tissues and cell lines was reported to regulate tumor cell proliferation, apoptosis, migration and invasion by targeting sirtuin-3 (15).…”

Section: Introductionmentioning

confidence: 99%

miR‑1225‑5p inhibits non‑small cell lung cancer cell proliferation, migration and invasion, and may be a prognostic biomarker

Zhang

2020

Exp Ther Med

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Non-small cell lung cancer (NSCLC) is a malignant tumor, which presents with a high 5-year mortality rate owing to the lack of an effective early screening tool and the absence of obvious early symptoms. MicroRNAs (miRs/miRNAs) have attracted increasing attention due to their significant clinical value in the diagnosis and prognosis of various human malignancies. The present study aimed to investigate the expression levels of microRNA (miR)-1225-5p in NSCLC and to analyze its prognostic value and biological role. The expression levels of miR-1225-5p in the tissues of patients with NSCLC and NSCLC cell lines were analyzed using reverse transcription-quantitative PCR. The association between miR-1225-5p expression levels and the clinicopathological features of patients with NSCLC was analyzed using a χ 2 test. The prognostic value of miR-1225-5p in NSCLC was analyzed using both Kaplan Meier survival and Cox regression analyses, and the effects of miR-1225-5p on NSCLC cell proliferation, migration and invasion were examined. The results revealed that the expression levels of miR-1225-5p were significantly downregulated in NSCLC tissues compared with normal control tissues. Furthermore, miR-1225-5p was discovered to be a potential independent prognostic factor in NSCLC. The inhibition of miR-1225-5p in NSCLC cell lines led to increased cell proliferation, migration and invasion, whereas miR-1225-5p overexpression exerted the opposite effects in these cells. In conclusion, the findings of the present study indicated that the downregulated expression levels of miR-1225-5p in NSCLC may predict a poor prognosis in patients and suggested miR-1225-5p as a potential therapeutic target for NSCLC.

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miR-1225-5p Functions as a Tumor Suppressor in Osteosarcoma by Targeting Sox9

Cited by 30 publications

References 21 publications

MiR-1225-5p acts as tumor suppressor in glioblastoma via targeting FNDC3B

MiR-1225-5p acts as tumor suppressor in glioblastoma via targeting FNDC3B

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

miR‑1225‑5p inhibits non‑small cell lung cancer cell proliferation, migration and invasion, and may be a prognostic biomarker

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