MiR‐1224‐5p acts as a tumor suppressor via inhibiting the malignancy of rectal cancer through targeting SLC29A3

Abstract

The emphasis of our study was to determine the physiological function of miR‐1224‐5p in rectal cancer (RC) and its in‐depth mechanism. First, the expression of miR‐1224‐5p in RC tissues was analyzed using public data from the TCGA database. Then, miR‐1224‐5p expression in RC cell lines SW480 and SW837 was measured using the qRT‐PCR assay. The subsequent CCK‐8 assay was executed to assess the function of miR‐1224‐5p in the viability of the RC cell. Bioinformatics prediction prompted that SLC29A3 may be a potential target gene for miR‐1224‐5p. Western blotting and dual‐luciferase reporter assays were performed to affirm the above forecasting. Kaplan–Meier analysis and Cox multivariate analysis were carried out to assess the relationship between SLC29A3 and prognosis. Finally, CCK‐8, colony formation assay, and transwell assay were used for functional analysis of miR‐1224‐5p/ SLC29A3 axis in vitro. MiR‐1224‐5p was expressed at low levels in RC tissues and cell lines. Up‐regulation of miR‐1224‐5p inhibited SW480 cell viability, while inhibition of miR‐1224‐5p enhanced the viability of SW837 cells. What is more, we affirmed that miR‐1224‐5p could direct target SLC29A3, which was expressed at high levels in RC tissues. In addition, SLC29A3 could be used as an independent predictive factor of prognosis in patients with RC, and the higher SLC29A3 expression, the lower survival rate. Finally, cellular functional experiments evidenced that miR‐1224‐5p mimic can reduce the cell viability, invasion, and migration, while overexpression of SLC29A3 presented an opposite effect. Importantly, co‐transfection experiments indicated that SLC29A3 can reverse miR‐1224‐5p‐mediated inhibition in the malignant progression of RC cells. Our work raised the possibility that miR‐1224‐5p functioned as a tumor suppressor in RC, which achieved its function via targeting SLC29A3.

Circ-RNF121 regulates tumor progression and glucose metabolism by miR-1224-5p/FOXM1 axis in colorectal cancer

“…Li et al have already indicated that miR-1224-5p expression was decreased in CRC patients, and its mimic repressed CRC cell metastasis [ 21 ]. Song et al also showed miR-1224-5p suppressed cell proliferation, migration and invasion in CRC [ 33 ]. In this research, it was found that miR-1224-5p expression was significantly downregulated in CRC tissues and cells.…”

IGFL2‐AS1 facilitates tongue squamous cell carcinoma progression via Wnt/β‐catenin signaling pathway

“…We identified that IGFL2-AS1 could interact with miR-1224-5p in TSCC cells. It has been reported that miR-1224-5p acts as a tumor suppressor in many cancers, such as glioma (Qian et al, 2015), ovarian cancer (Wang et al, 2020) and rectal cancer (Song et al, 2020).…”

“…We identified that IGFL2‐AS1 could interact with miR‐1224‐5p in TSCC cells. It has been reported that miR‐1224‐5p acts as a tumor suppressor in many cancers, such as glioma (Qian et al, 2015), ovarian cancer (Wang et al, 2020) and rectal cancer (Song et al, 2020). Similarly, our study found that miR‐1224‐5p was lowly expressed in TSCC cells, suggesting that miR‐1224‐5p might exert tumor suppressing function in TSCC progression.…”

Exosomal-mediated transfer of APCDD1L-AS1 induces 5-fluorouracil resistance in oral squamous cell carcinoma via miR-1224-5p/nuclear receptor binding SET domain protein 2 (NSD2) axis

“…Numerous microRNAs (miRNAs) have been identified as vital regulators in human cancers [ 40 ]. MiR-1224-5p played an anti-cancer role in various cancers, such as rectal cancer [ 41 ], pancreatic cancer [ 42 ], ovarian cancer [ 43 ], and glioma [ 44 ]. In addition, a report from Shi et al also elucidated that miR-1224-5p could impair the development of esophageal squamous cell carcinoma, which is another HNSC subtype [ 45 ].…”